Elucidation of Adult Newt Cells Regulating the ZRS enhancer during Limb Regeneration

Research Project

Project/Area Number	24K12150
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 55060:Emergency medicine-related
Research Institution	University of Tsukuba
Principal Investigator	CASCO.ROBLES Martin.Miguel 筑波大学, 生命環境系, 助教 (50874294)
Project Period (FY)	2024-04-01 – 2027-03-31
Project Status	Granted (Fiscal Year 2024)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2026: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2025: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2024: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	Limb / Patterning / ZRS / Shh / Regeneration
Outline of Research at the Start	Part 1: Isolation, Characterization of the newt ZRS and in vivo reporter of nZRS-hminimal promoter- mCherry for transgenic newts (Cynops pyrrhogaster). Part 2: Identification of adult ZRS cells in the newt using Cre/loxP tracking Part 3: Confirmation of ZRS positive cells effect on patterning.