|Budget Amount *help
¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2015: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2014: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2013: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
|Outline of Final Research Achievements
In this research, basic technologies are proposed and investigated in order to build up the liposome integrated biosensor chip for diagnosis, based on our previous liposome biosensor and relating sensing technologies that made clear their operation principles. The research consists of three parts: I. Physical control of liposome supramolecule or its droplet, II. Challenge for realizing amyloidosis sensor, and III. Creation of information processing electronic system for array sensor signal. Especially, amyloid-beta is focused as biomarker of Altzheimer’s disease from important target biomolecules for diagnosis. Consequently, the aggregation and fibrilization of the protein was detected selectively in human serum by the developed liposome sensor. Also, the sensitivity was markedly improved as low as 1 nM by selecting proper lipid species and applying low noise electronic circuit techniques.