The novel post-transcriptional regulation mechanism of antiviral host defense

• Project/Area Number: 25253030
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Hokkaido University
• Principal Investigator: Takaoka Akinori 北海道大学, 遺伝子病制御研究所, 教授 (30323611)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥45,630,000 (Direct Cost: ¥35,100,000、Indirect Cost: ¥10,530,000); Fiscal Year 2017: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000); Fiscal Year 2016: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2015: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000); Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2013: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: 自然免疫 / ウイルス感染 / 抗ウイルス効果 / RNA転写後制御 / RNA 転写後調節 / 免疫学 / 転写後調節 / RNAサイレンシング

Outline of Final Research Achievements

Viral infection is one of the most critical problems for our health. Elucidation of host immune response against invading viruses is crucial for better understanding of the pathological processes and viral elimination to control viral infection. In this study, we reveal that PARP-13 shorter isoform (ZAPS) is involved in the post-transcriptional regulation of RSAD2 mRNA, one of the interferon-stimulated genes, which provide a novel host defense mechanism for elimination of viruses.

Research Products

• [Journal Article] TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP). (2017)
  • Author(s): YLi MM, Lau Z, Cheung P, Aguilar EG, Schneider WM, Bozzacco L, Molina H, Buehler E, Takaoka A, Rice CM, Felsenfeld DP, MacDonald MR
  • Journal Title: PLoS Pathog Volume: 13 Issue: 1 Pages: e1006145-e1006145
  • DOI: 10.1371/journal.ppat.1006145
  • Open Access
• [Journal Article] Essential role of HCMV deubiquitinase in promoting oncogenesis by targeting anti-viral innate immune signaling pathways. (2017)
  • Author(s): Kumari P, Saha I, Narayanan A, Narayanan S, Takaoka A, Kumar NS, Tailor P, Kumar H.
  • Journal Title: Cell Death Dis. Volume: 8 Issue: 10 Pages: e3078-e3078
  • DOI: 10.1038/cddis.2017.461
• [Journal Article] Constitutive aryl hydrocarbon receptor signaling constrains type I interferon-mediated antiviral innate defense. (2016)
  • Author(s): Yamada T, Horimoto H, Kameyama T, Hayakawa S, Yamato H, Dazai M, Takada A, Kida H, Bott D, Zhou AC, Hutin D, Watts TH, Asaka M, Matthews, Takaoka A
  • Journal Title: Nat Immunol. Volume: 印刷中 Issue: 6 Pages: 687-694
  • DOI: 10.1038/ni.3422
  • NAID: 120005849912
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] <i><b>Helicobacter pylori</b></i><b> induces IL-1β protein through the inflammasome activation in differentiated macrophagic </b><b>cells </b> (2016)
  • Author(s): Kameoka, S.
  • Journal Title: Biomedical Research Volume: 37 Issue: 1 Pages: 21-27
  • DOI: 10.2220/biomedres.37.21
  • NAID: 130005128374
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access
• [Journal Article] Superoxide Dismutase 1 Protects Hepatocytes from Type I Interferon-Driven Oxidative Damage. (2015)
  • Author(s): Bhattacharya A, Hegazy AN, Deigendesch N, Kosack L, Cupovic J, Kandasamy RK, Hildebrandt A, Merkler D, K&#252;hl AA, Vilagos B, Schliehe C, Panse I, Khamina K, Baazim H, Arnold I, Flatz L, Xu HC, Lang PA, Aderem A, Takaoka A, Superti-Furga G, Colinge J, Ludewig B, L&#246;hning M, Bergthaler A.
  • Journal Title: Immunity Volume: 43 Issue: 5 Pages: 974-986
  • DOI: 10.1016/j.immuni.2015.10.013
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The microRNA miR-485 targets host and influenza virus transcripts to regulate antiviral immunity and restrict viral replication. (2015)
  • Author(s): Ingle H, Kumar S, Raut AA, Mishra A, Kulkarni DD, Kameyama T, Takaoka A, Akira S, Kumar H.
  • Journal Title: Science Signaling Volume: 8 Issue: 406
  • DOI: 10.1126/scisignal.aab3183
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The RNA Sensor RIG-I Dually Functions as an Innate Sensor and Direct Antiviral Factor for Hepatitis B Virus (2015)
  • Author(s): Sato S, Li K, Kameyama T, Hayashi T, Ishida Y, Murakami S, Watanabe T, Iijima S, Sakurai Y, Watashi K, Tsutsumi S, Sato Y, Akita H, Wakita T, Rice CM, Harashima H, Kohara M, Tanaka Y, Takaoka A
  • Journal Title: Immunity Volume: 42 Issue: 1 Pages: 123-132
  • DOI: 10.1016/j.immuni.2014.12.016
  • Peer Reviewed / Open Access
• [Presentation] Innate sensor-mediated IFN antiviral response and its regulatory mechanism (2016)
  • Author(s): 髙岡晃教
  • Organizer: Einladung zum gemeinsamen COLLOQUIUM (Invited Speaker Seminar)
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2016-02-25
  • Int'l Joint Research / Invited
• [Presentation] Tumor suppressive effect driven by cytoplasmic RNA-mediated innate signaling (2015)
  • Author(s): 髙岡晃教
  • Organizer: 8th Euro Global Summit on Cancer Therapy
  • Place of Presentation: Valencia,Spain
  • Year and Date: 2015-11-03
  • Int'l Joint Research
• [Presentation] Dual fection of RIG-I RNA helicase as an innate sensor and as a direct antiviral effector during hepatitis B virus infection (2015)
  • Author(s): 髙岡晃教
  • Organizer: 15th International Symposium on Viral Hepatitis and Liver Diseases
  • Place of Presentation: Berlin, Germany.
  • Year and Date: 2015-06-26
  • Int'l Joint Research
• [Presentation] CYTOPLASMIC RNA SENSOR RIG-I DUALLY MEDIATES ANTIVIRAL ACTIVITIES DURING HBV INFECTION (2015)
  • Author(s): 髙岡晃教
  • Organizer: 2nd World Conference on Targeting Liver Diseases
  • Place of Presentation: St Julian's, Malta
  • Year and Date: 2015-06-25
  • Int'l Joint Research
• [Presentation] NLRP3 recognizes Hericobacter pylori CagA protein to induce IL-1beta response (2015)
  • Author(s): 髙岡晃教
  • Organizer: 12th International Conference on Innate Immunity
  • Place of Presentation: Chania, Crete, Greece
  • Year and Date: 2015-06-19
  • Int'l Joint Research
• [Presentation] Direct and indirect roles of RIG-I for antiviral defense against hepatitis B virus in human hepatocytes (2014)
  • Author(s): Akinori Takaoka
  • Organizer: International Conference on Antimicrobial Research
  • Place of Presentation: Madrid (Spain)
  • Year and Date: 2014-10-01 – 2014-10-03
• [Presentation] Role of the cytoplasmic RNA sensor RIG-I in innate immune defense against hepatitis B virus infection (2014)
  • Author(s): Akinori Takaoka
  • Organizer: 11th International Conference on Innate Immunity
  • Place of Presentation: Olympia (Greece)
  • Year and Date: 2014-06-01 – 2014-06-06
• [Presentation] ZAPS acts as a key factor of RIG-I-mediated activation of innate immune responses during influenza virus (2013)
  • Author(s): Akinori Takaoka
  • Organizer: 2nd International Conference on Clinincal & Cellular Immunology
  • Place of Presentation: Hampton Inn Tropicana, Las Vegas USA
• [Presentation] Innate immune signaling networks during host-microbe interaction (2013)
  • Author(s): Akinori Takaoaka
  • Organizer: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
  • Place of Presentation: CeMM Vienna Austria
  • Invited
• [Book] Characterization of innate immune signalings stimulated by nucleic acid sensors. (2014)
  • Author(s): Kameyama, T. Takaoka, A
  • Publisher: Method in Mol. Biology.
• [Book] Arthritis Research Methods in Molecular Biology "Characterization of innate immune signalings stimulated by nucleic acid sensors" (2014)
  • Author(s): Takeshi Kameyama, Akinori Takaoka
  • Total Pages: 14
  • Publisher: Human Press
• [Remarks] ライフサイエンス 新着論文レビュー
  • URL: http://first.lifesciencedb.jp/archives/9761