| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
DNA damages caused by environmental genotoxic agents and ionizing radiations induce genome instability. Genome instability is a cause of cancer and cellular senescence, and thus understanding of the genome maintenance system is important issue. Here we defined genome maintenance system including chromatin regulation and DNA repair as ‘Genome maintenance machinery’ and studied this by the use of genetic approach and proteomics approach. To this end, we applied SILAC technique to DT40 DNA repair mutant panel. Our comprehensive approach revealed the relationship among signal pathway, repair factor and chromatin modifications. In this report, we explain about the identification of ubiquitination target proteins of RNF4 ubiquitin ligase.
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