Prolonged functional lifespan of artificial red cells by using the electron energies produced by erythrocyte glycolysis
| Project/Area Number |
25282136
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Nara Medical University |
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Principal Investigator |
SAKAI HIROMI 奈良県立医科大学, 医学部, 教授 (70318830)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Keywords | 人工酸素運搬体 / 人工赤血球 / リポソーム / ヘモグロビン / 電子伝達物質 / メトヘモグロビン / 輸血代替 / 酸素輸送 / 電子伝達系 / 解糖系 / 血液代替物 / Phenothiazine / 人工血液 / 再生医学 / 生体機能利用 / 生体材料 / 電子移動 / レドックス / ナノバイオ |
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| Outline of Final Research Achievements |
In vivo safety and efficacy of artificial red cells (Hb-vesicles, Hb-V) as a transfusion alternative have been clarified extensively. Gradual autoxidation of ferrous Hb in Hb-V to form ferric metHb is inevitable and results in decreased oxygen carrying capacity. We found that metHb in Hb-V can be reduced to ferrous Hb by using the electron energies produced by erythrocyte glycolysis, via methylene blue as a transmembrane electron mediator. We tested other 14 candidate mediators, and found some other potential molecules that can reduce metHb effectively, as did methylene blue. The requisite chemical properties as a potential electron mediator are clarified. We established an indirect enzymatic metHb reducing system for Hb-V using unlimited endogenous electrons created in RBCs in combination with an effective electron mediator that prolongs the functional lifespan of HbV in blood circulation.
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Report
(4 results)
Research Products
(14 results)
