Development of a method for the epigenetic analysis of single cells under a micro fluidic device
Project/Area Number |
25286035
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Nano/Microsystems
|
Research Institution | The University of Tokyo |
Principal Investigator |
Oana Hidehiro 東京大学, 工学(系)研究科(研究院), 准教授 (20314172)
|
Co-Investigator(Renkei-kenkyūsha) |
WASHIZU MASAO 東京大学, 大学院工学系研究科, 教授 (10201162)
OKEYO KENNEDY 東京大学, 大学院工学系研究科, 助教 (10634652)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
|
Keywords | ゲノム / DNA / マイクロ流体デバイス / バイオテクノロジー / マイクロ・ナノデバイス / シングルセル / エピジェネティクス |
Outline of Final Research Achievements |
We developed a new methodology for analysis of individual intact chromosomes/chromatins isolated from single cells. We developed a custom-designed microfluidic device for isolation, control of the higher order structure of individual chromosome/chromatin by changing solution conditions under a fluorescence microscope. This device is also able to perform immunofluorescence staining. The method developed here has a potential application for direct investigation of the epigenetic profile of intact chromatin fibers at individual cell level.
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Report
(4 results)
Research Products
(13 results)