| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
|
|---|
| Outline of Final Research Achievements |
In order to measure the translational diffusion coefficient change by the transient grating method combined with a stopped flow system, we constructed a new stopped flow system for minimizing turbulence after mixing two solutions. This system can suppress the turbulence within 100 ms after the mixing. Furthermore, we improved the system to decrease a volume of required sample solution, and succeeded in mixing only a few micro liter solution, which is a big advantage for application to protein samples. By using this system, we measured a diffusion coefficient change in time-domain after acid denaturation of a photosensor protein of phototropin sample. Moreover, association reaction of a protein of KaiC which has a function to control the circadian rhythm was detected in time-domain.
|
