Development of an exhaustive detection technique for viable waterborne bacteria by using fluorescence activated cell sorter and next-generation sequencing
Project/Area Number |
25289176
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Civil and environmental engineering
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Research Institution | National Institute of Public Health |
Principal Investigator |
Akiba Michihiro 国立保健医療科学院, その他部局等, その他 (00159336)
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Co-Investigator(Kenkyū-buntansha) |
岸田 直裕 国立保健医療科学院, その他部局等, その他 (10533359)
常田 聡 早稲田大学, 理工学術院, 教授 (30281645)
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Research Collaborator |
FUJIMOTO Naoshi 東京農業大学, 応用生物科学部醸造科学科, 教授 (50297595)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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Keywords | 水道 / 微生物 / 遺伝子検査 |
Outline of Final Research Achievements |
In this study, we aimed to develop an exhaustive detection technique for viable waterborne bacteria by using fluorescence activated cell sorter (FACS) and next-generation sequencing (NGS). The developed FACS-NGS method was evaluated using process water samples collected in a drinking water treatment plant, comparing with a conventional cloning and Sanger sequencing (culture-dependent) method. As a result, microbial communities observed in the process water samples were different between the FACS-NGS method and culture-dependent method. Alphaproteobacteria was the major class determined by the culture-dependent method. In contrast, Alphaproteobacteria and Gammaproteobacteria were identified as the two major classes in the FACS-NGS method. The results suggested that the developed FACS-NGS method can detect diverse viable waterborne bacteria.
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Report
(5 results)
Research Products
(13 results)
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[Journal Article] Community analysis of picocyanobacteria in an oligotrophic lake by cloning 16S rRNA gene and 16S rRNA gene amplicon sequencing2015
Author(s)
Fujimoto N, Mizuno K, Yokoyama T, Ohnishi A, Suzuki M, Watanabe S, Komatsu K, Sakata Y, Kishida N, Akiba M, Matsukura S.
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Journal Title
The Journal of General and Applied Microbiology
Volume: 61
Issue: 5
Pages: 171-176
DOI
NAID
ISSN
0022-1260, 1349-8037
Related Report
Peer Reviewed / Open Access
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[Presentation] 水中微生物検査のありかた2014
Author(s)
岸田直裕
Organizer
日本防菌防黴学会第41回年次大会
Place of Presentation
東京
Year and Date
2014-09-24 – 2014-09-25
Related Report
Invited
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