Molecular basis of spinal glia/endothelium/neurons interaction as the mechanisms of development and maintenance of chronic pain
| Project/Area Number |
25290016
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Nerve anatomy/Neuropathology
|
|---|
| Research Institution | Hyogo Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YAGI Hideshi 兵庫医科大学, 医学部, 教授 (10303372)
YAMANAKA Hiroki 兵庫医科大学, 医学部, 講師 (20340995)
KOBAYASHI Kimiko 兵庫医科大学, 医学部, 講師 (70418961)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
|
|---|
| Keywords | 神経障害性疼痛 / グリア / 脂質メディエーター / ニューロン / マイクログリア / プロスタグランジン / 合成酵素 / Rho / ROCK / p38 |
|---|
| Outline of Final Research Achievements |
Using rat neuropathic pain models, we found the following findings.
1. PGD2 produced by microglia is COX-1 dependent, and neurons in the spinal cord can receive PGD2 from microglia following peripheral nerve injury, suggesting a role of PGD2 signaling in mechanical allodynia. 2. We found the details of RhoA/ROCK pathway in spinal microglia in this model. It mediates p38 MAPK activation and morphological changes downstream of P2Y12/13 receptors in spinal microglia in neuropathic pain. 3. We found that increased artemin in peripheral tissues plays an important role on the regulation of TRPV1/A1 in DRG neurons in pathological conditions such as inflammatory and neuropathic pain. 4. Using electrophysiological method, we found leukotriene enhances NMDA-induced inward currents in dorsal horn neurons of the rat spinal cord after peripheral nerve injury. 5. We found that microglial TNF alpha is able to induce COX2 and PGI2 synthase expression in spinal endothelial cells during neuropathic pain.
|
Report
(4 results)
Research Products
(32 results)
-
-
-
-
-
-
-
-
-
-
[Journal Article] Filamin A-interacting protein (FILIP) is a region-specific modulator of myosin 2b and controls spine morphology and NMDA receptor accumulation.2014
Author(s)
Hideshi Yagi,Takashi Nagano,Min-Jue Xie,Hiroshi Ikeda,Kazuki Kuroda,Munekazu Komada,Tokuichi Iguchi,Rahman M. Tariqur,Soichi Morikubo,Koichi Noguchi,Kazuyuki Murase,Masaru Okabe& Makoto Sato
-
Journal Title
Scientific reports
Volume: 4
Issue: 1
Pages: 6353-6353
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Xilei san ameliorates experimental colitis in rats by selectively degrading proinflammatory mediators and promoting mucosal repair.2014
Author(s)
Hao Y, Nagase K, Hori K, Wang S, Kogure Y, Fukunaga K, Kashiwamura S, Yamamoto S, Nakamura S, Li J, Miwa H, Noguchi K, Dai Y.
-
Journal Title
Evid Based Complement Alternat Med
Volume: 2014
Issue: 1
Pages: 569587-569587
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
