• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the regulatory network of cohesin acetylation

Research Project

Project/Area Number 25290065
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Genome biology
Research InstitutionThe University of Tokyo

Principal Investigator

Bando Masashige  東京大学, 分子細胞生物学研究所, 助教 (90360627)

Co-Investigator(Kenkyū-buntansha) NAKATO Ryuichiro  東京大学, 分子細胞生物学研究所, 助教 (60583044)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywordsコヒーシン / アセチル化 / Esco / 姉妹染色分体間接着 / DNA複製 / MCMヘリカーゼ / ゲノム / Mcmヘリカーゼ / クロマチン / 姉妹染色体 / 転写制御
Outline of Final Research Achievements

There are two cohesin acetyl transferase, Esco1 and Esco2, in human. Both of them are essential for establishment of sister chromatids cohesion. We found that Esco1 directly binds to cohesin subunit Pds5 and co-localizes with cohesin. The domain for binding with Pds5 is responsible for cohesin acetylation and cohesion establishment. Esco1 phosphorylated in mitosis prevents its binding with Pds5. Therefore phosphorylation may have important role in controlling Esco1 localization. Esco2 associates with MCM helicases during G1 to S-phase. The N-terminal domain of Esco2 conserved among vertebrates is required for direct interation with MCM. It has been known that Esco2 is down-regulated from late G2 to M phase. Interestingly Esco2 mutant that is unable to bind to Mcm is destabilized. This destabilization of Esco2 is caused by proteaosome pathway mediated by Cul4 ligase. Thus, Esco1 and Esco2 function in different pathway from each other to promote cell cycle progression.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (14 results)

All 2015 2014 2013

All Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 5 results,  Acknowledgement Compliant: 3 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Condensin targets and reduces unwound DNA structures associated with transcription in mitotic chromosome condensation2015

    • Author(s)
      Takashi Sutani, Toyonori Sakata, Ryuichiro Nakato, Koji Masuda, Mai Ishibashi, Daisuke Yamashita, Yutaka Suzuki, Tatsuya Hirano, Masashige Bando, Katsuhiko Shirahige
    • Journal Title

      Nature Communications

      Volume: 6 Issue: 1 Pages: 1-13

    • DOI

      10.1038/ncomms8815

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Esco1 Acetylates Cohesin via a Mechanism Different from That of Esco22015

    • Author(s)
      Masashi Minamino, Mai Ishibashi, Ryuichiro Nakato, Kazuhiro Akiyama, Hiroshi Tanaka, Yuki Kato, Lumi Negishi, Toru Hirota, Takashi Sutani, Masashige Bando, Katsuhiko Shirahige
    • Journal Title

      Current Biology

      Volume: 25 Issue: 13 Pages: 1694-1706

    • DOI

      10.1016/j.cub.2015.05.017

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Deubiquitinating Enzyme USP7 Regulates Androgen Receptor Activity by Modulating Its Binding to Chromatin2015

    • Author(s)
      Shu-Ting Chen, Maiko Okada, Ryuichiro Nakato, Kosuke Izumi, Masashige Bando and Katsuhiko Shirahige
    • Journal Title

      Journal of Biological Chemistry

      Volume: 290 Issue: 35 Pages: 21713-21723

    • DOI

      10.1074/jbc.m114.628255

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin.2015

    • Author(s)
      Izumi K, Nakato R, Zhang Z, Edmondson AC, Noon S, Dulik MC, Rajagopalan R, Venditti CP, Gripp K, Samanich J, Zackai EH, Deardorff MA, Clark D, Allen JL, Dorsett D, Misulovin Z, Komata M, Bando M, Kaur M, Katou Y, Shirahige K*, Krantz ID*.
    • Journal Title

      Nature Genetics

      Volume: 47 Issue: 4 Pages: 338-338

    • DOI

      10.1038/ng.3229

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chromatin immunoprecipitation protocol for mammalian cells.2014

    • Author(s)
      Komata M, Katou Y, Tanaka H, Nakato R, Shirahige K, Bando M.
    • Journal Title

      Methods Mol Biol.

      Volume: 1164 Pages: 33-38

    • DOI

      10.1007/978-1-4939-0805-9_4

    • ISBN
      9781493908042, 9781493908059
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Design, Synthesis, and Biological Activity of NCC149 Derivatives as Histone Deacetylase 8-Selective Inhibitors.2014

    • Author(s)
      Suzuki T, Muto N, Bando M, Itoh Y, Masaki A, Ri M, Ota Y, Nakagawa H, Iida S, Shirahige K, Miyata N.
    • Journal Title

      ChemMedChem.

      Volume: 9 Issue: 3 Pages: 657-664

    • DOI

      10.1002/cmdc.201300414

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells.2014

    • Author(s)
      Gallego-Paez LM, Tanaka H, Bando M, Takahashi M, Nozaki N, Nakato R, Shirahige K*, Hirota T*.(Shared Corresponding Authors)
    • Journal Title

      Mol Biol Cell.

      Volume: 27 Issue: 2 Pages: 2736-2748

    • DOI

      10.1091/mbc.e13-01-0020

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Loss-of-function HDAC8 mutations cause a phenotypic spectrum of Cornelia de Lange syndrome-like features, ocular hypertelorism, large fontanelle and X-linked inheritance.2014

    • Author(s)
      Kaiser FJ, Ansari M, Braunholz D, et al
    • Journal Title

      Hum Mol Genet.

      Volume: Jan Issue: 11 Pages: 1-13

    • DOI

      10.1093/hmg/ddu002

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.2013

    • Author(s)
      Kon A, Yoshida K
    • Journal Title

      Nature Genet.

      Volume: 45 Issue: 10 Pages: 1232-1237

    • DOI

      10.1038/ng.2731

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] The role of cohesin and cohesin loader in transcripitional regulation2015

    • Author(s)
      Masashige Bando, Ryuichiro Nakato, Kazuhiro Akiyama, Hiroshi Tanaka, Yuki Katou, Katsuhiko Shirahige
    • Organizer
      EMBO workshop ‘SMC proteins’
    • Place of Presentation
      siena, Italy
    • Year and Date
      2015-05-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The role of Cohesin loader in transcripitional regulation2014

    • Author(s)
      Masashige Bando, Ryuichiro Nakato, Kazuhiro Akiyama, Hiroshi Tanaka, Yuki Katou, Katsuhiko Shirahige
    • Organizer
      The 4D nucleosome 2014
    • Place of Presentation
      広島
    • Year and Date
      2014-12-17 – 2014-12-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 転写調節におけるコヒーシンローダーの機能解析2014

    • Author(s)
      坂東優篤、中戸隆一郎、秋山和弘、田中博志、加藤由起、白髭克彦
    • Organizer
      日本分子生物学会第38年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-29 – 2014-12-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] Interacting networks of cohesin and cohesin loader2013

    • Author(s)
      Makiko Komata, Katsuhiko Shirahige,Masashige Bando
    • Organizer
      Cohesin Biology and the Cohesinopathies
    • Place of Presentation
      Siena, Italy
    • Related Report
      2013 Annual Research Report
  • [Book] 細胞工学 染色体工学Update 2015 vol.34.11月2015

    • Author(s)
      坂東優篤、秋山和弘、白髭克彦
    • Total Pages
      5
    • Publisher
      細胞工学 学研メディカル秀潤社
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi