Structural basis for multidrug tripartite efflux pump through both the inner and outer membranes in Pseudomonas aeruginosa.
| Project/Area Number |
25291014
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
Yamashita Eiki 大阪大学, たんぱく質研究所, 助教 (00294132)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA ATSUSHI 大阪大学, 蛋白質研究所, 教授 (20188890)
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| Research Collaborator |
YONEHARA RYO 大阪大学, 蛋白質研究所, 研究員
IKEDA ETSUKO 大阪大学, 蛋白質研究所, 博士研究員
堤 研太 大阪大学, 蛋白質研究所, 博士前期課程
HIGA YUKI 大阪大学, 蛋白質研究所, 博士前期課程
SUMITA KAZUMA 大阪大学, 蛋白質研究所, 博士前期課程
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Keywords | X線結晶構造解析 / 膜タンパク質複合体の物質輸送 / 外膜チャンネルの構造 |
|---|
| Outline of Final Research Achievements |
The multidrug resistance of Pseudomonas aeruginosa is mainly attributable to high expression of the tripartite efflux pumps that transport antibiotics from within to the external environment. The tripartite efflux pumps, each of which is composed of an inner membrane transporter, a periplasmic adaptor protein and an outer membrane factor, are membrane-protein super-complexes spanning both the inner and outer membranes. In this study, we determined the stoichiometry of the tripartite efflux pump by size exclusion chromatography and gel electrophoresis and the structure of it by negative stain electron microscope. The structures of two major outer membrane factors from multidrug-resistance Pseudomonas aeruginosa were determined by X-ray crystallographic analysis.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] A new protein complex promoting the assembly of Rad51 filaments.2013
Author(s)
Sasanuma H., Tawaramoto M. S., Lao J. P., Hosaka H., Sanda E., Suzuki M., Yamashita E., Hunter N., Shinohara M., Nakagawa A., Shinohara A.
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Journal Title
Nat. Commun.
Volume: 4
Issue: 1
Pages: 1676-1676
DOI
Related Report
Peer Reviewed
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[Presentation] Crystallography using Synchrotron Radiation2015
Author(s)
Atsushi Nakagawa
Organizer
Joint Symposium between the Institute for Protein Research (Osaka University) and the Research School of Chemistry (Australian National University) "Protein Structure and Function”
Place of Presentation
Canberra（Australia）
Year and Date
2015-11-14
Related Report
Int'l Joint Research / Invited
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[Presentation] IPR Beamline for Macromolecular Assemblies at SPring-8 (BL44XU)2014
Author(s)
Eiki Yamashita, Akifumi Higashiura, Masato Yoshimura, Kazuya Hasegawa, Yukito Furukawa, Toru Ohata, Takashi Kumasaka, Go Ueno, Masaki Yamamoto, Atsushi Nakagawa
Organizer
23rd Congress and General Assembly of the International Union of Crystallography
Place of Presentation
Montreal
Year and Date
2014-08-05 – 2014-08-12
Related Report
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[Presentation] Coherent Diffraction Imaging for Structure Determination for Spherical Biological Particles2013
Author(s)
Atsushi Nakagawa, Akifumi Higashiura, Kenji Iwasaki, Marina Murakami, Eiki Yamashita, Kazuki Takeda, Yu Hirano, Yuya Hanazono, Kiyofumi Takaba, Masahito Hibi, Yukiko Tomisaki, Kunio Miki
Organizer
The 12th Conference of the Asian Crystallographic Association
Place of Presentation
Hong Kong University of Science and Technology, Hong Kong
Related Report
Invited
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