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Molecular mechanisms of organelle biogenesis using autophagosome formation as a model system

Research Project

Project/Area Number 25291040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Cell biology
Research InstitutionThe University of Tokyo

Principal Investigator

Suzuki Kuninori  東京大学, 新領域創成科学研究科, 准教授 (20373194)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsオートファジー / タンパク質分解 / オルガネラ分解 / 出芽酵母 / オルガネラ / Atgタンパク質 / 生体膜 / 栄養飢餓 / ストレス / オートファゴソーム / 小胞体 / ER exit site / 膜輸送 / オルガネラ形成
Outline of Final Research Achievements

Autophagy is a highly conserved cellular recycling process involved in degradation of eukaryotic cellular components. During autophagy, macromolecules and organelles are sequestered into the double-membrane autophagosome and degraded in the vacuole/lysosome. Atg3 is an E2-like enzyme that catalyzes the conjugation reaction between Atg8 and phosphatidylethanolamine (PE). We constructed functional GFP-tagged Atg3 (Atg3-GFP) by inserting the GFP portion immediately after the handle region of Atg3. During autophagy, Atg3-GFP transiently formed a single dot per cell on the vacuolar membrane. This Atg3-GFP dot colocalized with 2×mCherry-tagged Atg8, demonstrating that Atg3 is localized to autophagic structures. Furthermore, we found that Atg3-GFP is localized to the IM by fine-localization analysis. The localization of Atg3 suggests that Atg3 plays an important role in autophagosome formation at the IM.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (17 results)

All 2016 2015 2014 2013 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)2016

    • Author(s)
      Klionsky DJ., Abdelmohsen K., Abe A., Abedin MJ.,Inomata M., et al.
    • Journal Title

      Autophagy

      Volume: 12(1) Issue: 1 Pages: 1-222

    • DOI

      10.1080/15548627.2015.1100356

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Visualization of Atg3 during autophagosome formation in Saccharomyces cerevisiae2015

    • Author(s)
      Meipin Ngu, Eri Hirata, and Kuninori Suzuki
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 290 Issue: 13 Pages: 8146-8153

    • DOI

      10.1074/jbc.m114.626952

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Proteomic profiling of autophagosome cargo in Saccharomyces cerevisiae2014

    • Author(s)
      Suzuki K, Nakamura S, Morimoto M, Fujii K, Noda NN, Inagaki F, Ohsumi Y.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 3 Pages: e91651-e91651

    • DOI

      10.1371/journal.pone.0091651

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae2013

    • Author(s)
      鈴木邦律他
    • Journal Title

      Journal of Cell Science

      Volume: 126 Pages: 2534-2544

    • DOI

      10.1242/jcs.122960

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Molecular Mechanisms of Autophagosome Formation in S. cerevisiae2016

    • Author(s)
      Kuninori Suzuki
    • Organizer
      14th International Congress on Yeasts
    • Place of Presentation
      淡路島夢舞台国際会議場(兵庫県淡路市)
    • Year and Date
      2016-09-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 出芽酵母のオートファゴソーム形成におけるAtg4の機能解析2015

    • Author(s)
      平田恵理,鈴木邦律
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] オートファゴソーム形成におけるAtg4の機能解析2015

    • Author(s)
      平田恵理,鈴木邦律
    • Organizer
      酵母遺伝学フォーラム第48回研究報告会
    • Place of Presentation
      広島大学東広島キャンパス(広島県東広島市)
    • Year and Date
      2015-08-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] 液胞内リパーゼ様タンパク質Atg15の機能解析2015

    • Author(s)
      白井亨,鈴木邦律
    • Organizer
      酵母遺伝学フォーラム第48回研究報告会
    • Place of Presentation
      広島大学東広島キャンパス(広島県東広島市)
    • Year and Date
      2015-08-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] 出芽酵母におけるオートファジックボディ分解機構の解析2015

    • Author(s)
      白井亨,鈴木邦律
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2015-07-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] オートファゴソーム形成におけるAtg4の機能解析2015

    • Author(s)
      平田恵理,鈴木邦律
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae2014

    • Author(s)
      鈴木邦律
    • Organizer
      第14回 日本タンパク質科学会年会
    • Place of Presentation
      ワークピア横浜
    • Year and Date
      2014-06-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] オートファジーによる被分解タンパク質の網羅的解析2013

    • Author(s)
      鈴木邦律他
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      ウインクあいち
    • Related Report
      2013 Annual Research Report
  • [Book] Autophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection, and Aging, 1st Edition: Autophagic Structures in Yeast2016

    • Author(s)
      Kuninori Suzuki
    • Total Pages
      16
    • Publisher
      Elsevier
    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京大学大学院新領域創成科学研究科 生命応答システム分野HP

    • URL

      http://ps.k.u-tokyo.ac.jp/

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 東京大学大学院新領域創成科学研究科 プロスペクタス

    • URL

      http://www.k.u-tokyo.ac.jp/pros/person/kuninori_suzuki/kuninori_suzuki.htm

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 東京大学大学院新領域創成科学研究科先端生命科学専攻生命応答システム分野HP

    • URL

      http://ps.k.u-tokyo.ac.jp/index.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 東京大学大学院新領域創成科学研究科プロスペクタス

    • URL

      http://www.k.u-tokyo.ac.jp/pros/person/kuninori_suzuki/kuninori_suzuki.htm

    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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