Molecular mechanism and physiological significance of alternative splicing regulation by phytochrome
| Project/Area Number |
25291064
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kyushu University |
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Principal Investigator |
Matsushita Tomonao 九州大学, (連合)農学研究科(研究院), 准教授 (20464399)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Keywords | フィトクロム / 光受容体 / 植物 / シグナル伝達 / 光応答 / 選択的スプライシング / 光シグナル伝達 / 光生理学 / シロイヌナズナ / 遺伝子発現制御 / 光信号伝達 / 光形態形成 |
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| Outline of Final Research Achievements |
At present, it is widely believed that phytochrome regulates the transcription of light-responsive genes by modulating the activity of several transcription factors. However, we performed mRNA-seq analysis and revealed that phytochrome controls not only transcription, but also alternative splicing, to approximately the same degree at the genomic level in Arabidopsis. Moreover, we experimentally showed that phytochrome-induced alternative splicing changes of SPA3, the negative regulator of light signaling, physiologically contributed to promoting light responses of plants. From these results, we demonstrated that phytochrome directly and simultaneously regulates two different aspects of gene expression, namely transcription and alternative splicing to mediate light responses in plants.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Phytochrome controls alternative splicing to mediate light responses in Arabidopsis.2014
Author(s)
Shikata, H., Hanada, K., Ushijima, T., Nakashima, M., Suzuki, Y. and Matsushita, T.
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Journal Title
Proc Natl Acad Sci USA
Volume: 111
Issue: 52
Pages: 18781-18786
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced stomatal opening in Arabidopsis thaliana2013
Author(s)
Ando E, Ohnishi M, Wang Y, Matsushita T, Watanabe A, Hayashi Y, Fujii M, Ma JF, Inoue S, and Kinoshita T
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Journal Title
Plant Physiology
Volume: 162
Pages: 1529-1538
Related Report
Peer Reviewed
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[Journal Article] New approach for fish breeding by chemical mutagenesis: establishment of TILLING method in fugu (Takifugu rubripes) with ENU mutagenesis2013
Author(s)
Kuroyanagi M, Katayama T, Imai T, Yamamoto Y, Chisada S, Yoshiura Y, Ushijima T, Matsushita T, Fujita M, Nozawa A, Suzuki Y, Kikuchi K, Okamoto H
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Journal Title
BMC Genomics
Volume: 14
Pages: 786-786
Related Report
Peer Reviewed
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