Genomic and structural studies on asymmetrical formation of male and female gametes in the gametogenesis of the macroalgae Ulva by using immune-subtraction
Project/Area Number |
25291070
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Morphology/Structure
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Research Institution | The University of Tokyo |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
MIYAMURA SHINICHI 筑波大学, 生命環境科学研究科, 准教授 (00192766)
KUWANO KAZUYOSHI 長崎大学, 大学院水産・環境科学総合研究科, 教授 (60301363)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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Keywords | 性染色体 / 微生物 / 藻類形態 / 雌雄性 / サブトラクション / アオノリ / 同形配偶子 / 非対称性 / アノノリ |
Outline of Final Research Achievements |
The genus Ulva is a green macroalga which has a haploid-diploid isomorphic life cycle. Its gametes show asymmetric positions of a mating structure and an eyespot between the opposite mating types. We analyzed whole genome sequences of two strains with opposite mating types (mt- and mt+) in Ulva to find that identical genomic regions homologous with GCS1. Immunofluorescence observations using an antibody against GCS1 demonstrated that its products localized to the cellular surface between the two flagella at a potential site for an mt- mating structure. Comprehensive comparison of a total of 12.0 Gbp and 16.6 Gbp of the sequencing read data for mt- and mt+ genomes identified MT loci of 1.0 and 1.5 Mbp in size containing 46 and 67 genes, respectively. The MT loci of these mating types shared 23 genes. These provide a new model that sheds light on the evolution of the mating locus with ancestral traits in multicellular organisms exhibiting haploid selection and low complexity.
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Report
(4 results)
Research Products
(96 results)
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[Journal Article] Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri.2016
Author(s)
Ota, S., Yoshihara, M., Yamazaki, T., Takeshita, T., Hirata, A., Konomi, M., Oshima, K., Hattori., M., Bišová, K.,
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Journal Title
Sci. Rep.
Volume: 6
Pages: -
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure.2016
Author(s)
Ota, S., Oshima, K., Yamazaki, T., Kim, S., Yu, Z., Yoshihara, M., Takeda, K., Takeshita,T., Hirata, A., Bišova, K., Zachleder, V., Hattori, M., and Kawano, S.
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Journal Title
Biotechnol. Biofuels.
Volume: 9
Pages: -
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Characterization of isolates derived from heavy-ion-beam irradiated cells in the unicellular green alga Parachlorella kessleri.2015
Author(s)
Yamazaki, T., Yamashita, Y., Ota, S., Takeshita, T., Kazama, Y., Abe, T., and Kawano, S.
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Journal Title
RIKEN Accel. Prog. Rep.
Volume: 48
Pages: 309
Related Report
Peer Reviewed / Open Access
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[Journal Article] Axillary Meristem Formation in Rice Requires the WUSCHEL Ortholog TILLERS ABSENT1.2015
Author(s)
Tanaka, W., Ohmori, Y., Ushijima, T., Matsusaka, H., Matsushita, T., Kumamaru, T., Kawano, S., and Hirano, H.Y.
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Journal Title
Plant Cell
Volume: 27
Pages: 1173-1184
DOI
Related Report
Peer Reviewed
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[Journal Article] Mutation rates of Parachlorella kessleri by heavy ion beam irradiation and classification of their genomic deletion types.2014
Author(s)
Yamazaki, T., Ota, S., Oshima, K. Hattori, M., Kazama, Y., Abe, T. and Kawano, S.
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Journal Title
RIKEN Accel. Prog. Rep.
Volume: 47
Pages: 301
Related Report
Peer Reviewed / Open Access
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[Journal Article] HAP2/GCS1 is involved in the sexual reproduction system of the marine macroalga Ulva compressa.2014
Author(s)
Yamazaki, T., Endo, M., Ito, K., Suzuki, R., Ota, S., Kuwano, K., Miyamura, S., Toyoda, A. and Kawano, S.
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Journal Title
Cytologia
Volume: 79
Pages: 575-584
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Use of FM1-43, a membrane-specific fluorescent dye, to estimate plasma membrane integrity in the cryopreservation of green algae.2014
Author(s)
Yamazaki, T., Hirai, C., Ota, S., Kuwano, K., and Kawano, S.
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Journal Title
Cryo Letters
Volume: 35
Pages: 180-187
Related Report
Peer Reviewed / Open Access
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[Journal Article] Starch and lipid accumulation in eight strains of six Chlorella species under strong illumination and aeration culture conditions.2014
Author(s)
Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., and Kawano, S.
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Journal Title
Bioresour. Technol.
Volume: 158
Pages: 127-134
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Use of FM1-43, a membrane-specific fluorescent dye, to estimate plasma membrane integrity in the cryopreservation of green algae.2014
Author(s)
Yamazaki, T., Hirai, C., Ota, S., Kuwano, K., and Kawano, S
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Journal Title
Cryo Letters
Volume: -
Pages: -
Related Report
Peer Reviewed
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[Journal Article] Rapid degeneration of noncoding DNA regions surrounding SlAP3X/Y after recombination suppression in a dioecious plant, Silene latifolia.2013
Author(s)
Ishii, K., Nishiyama, R., Shibata, F., Kazama, Y., Abe, T., and Kawano, S.
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Journal Title
G3
Volume: 3
Pages: 2121-2130
DOI
Related Report
Peer Reviewed
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[Journal Article] Image-based monitoring system for green algal Haematococcus pluvialis (Chlorophyceae) cells during culture.2013
Author(s)
Ohnuki, S., Nogami, S., Ota, S., Watanabe, K., Kawano, S., Ohya, Y.
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Journal Title
Plant Cell Physiol.
Volume: 54
Pages: 1917-1929
DOI
Related Report
Peer Reviewed
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[Journal Article] Phenotypic spectrum of Parachlorella kessleri (Chlorophyta) mutants produced by heavy-ion irradiation.2013
Author(s)
Ota, S., Matsuda, T., Takeshita, T., Yamazaki, T., Kazama, Y., Abe, T., Kawano, S.
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Journal Title
Bioresour. Technol.
Volume: 149
Pages: 432-438
DOI
Related Report
Peer Reviewed
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[Journal Article] Relationship between starch and lipid accumulation induced by nutrient depletion and replenishment in the microalga Parachlorella kessleri.2013
Author(s)
Fernandes, B., Teixeira, J., Dragone, G., Vicente, A.A., Kawano, S., Bisova, K., Pribyl, P., Zachleder, V., and Vitova, M.
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Journal Title
Bioresour. Technol.
Volume: 144
Pages: 268-274
DOI
Related Report
Peer Reviewed
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[Journal Article] Single-membrane-bounded peroxisome division revealed by isolation of dynamin-based machinery.2013
Author(s)
Imoto, Y., Kuroiwa, H., Yoshida, Y., Ohnuma, M., Fujiwara, T., Yoshida, M., Nishida, K., Yagisawa, F., Hirooka, S., Miyagishima, SY., Misumi, O., Kawano, S., Kuroiwa, T.
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Journal Title
Proc Natl Acad Sci USA.
Volume: 110
Pages: 9583-9588
DOI
Related Report
Peer Reviewed
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[Journal Article] Localization and evolution of septins in algae.2013
Author(s)
Yamazaki, T., Owari, S., Ota, S., Sumiya, N., Yamamoto, M., Watanabe, K., Nagumo, T., Miyamura, S. and Kawano, S.
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Journal Title
Plant J.
Volume: 74
Pages: 605-614
DOI
Related Report
Peer Reviewed
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[Journal Article] Three-dimensional ultrastructural study of oil and astaxanthin accumulation during encystment in the green alga Haematococcus pluvialis.2013
Author(s)
Wayama, M., Ota, S., Matsuura, M., Nango, N., Hirata, A., and Kawano, S.
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Journal Title
PLoS ONE
Volume: 8
Pages: e53618
DOI
Related Report
Peer Reviewed
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[Journal Article] Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species.2013
Author(s)
Mizuno, Y., Sato, A., Watanabe, K., Hirata, A., Takeshita, T., Ota, S., Sato, N., Zachleder, V., Tsuzuki, M., and Kawano, S.
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Journal Title
Bioresour. Technol.
Volume: 129
Pages: 150-155
DOI
Related Report
Peer Reviewed
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[Journal Article] The Microalga Parachlorella kessleri: A Novel Highly Efficient Lipid Producer.2013
Author(s)
Li, X., Pribyl, P., Binova, K., Kawano, S., Cepak, V., Zachleder, V., Cizkova, M., Branyikova, I., and Vitova, M.
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Journal Title
Biotechnol. Bioeng.
Volume: 110
Pages: 97-107
DOI
Related Report
Peer Reviewed
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[Presentation] The Parachlorella genome provides insight into trebouxiophycean evolution and oil production traits for biofuel potential.2015
Author(s)
Ota, S., Oshima, K., Yamazaki, T., Kim, S., Yu, Z., Yoshihara, M., Takeda, K., Takeshita1, T., Hirata, A., Bišova, K., Zachleder, V., Hattori, M., and Kawano, S.
Organizer
6th European Phycological Congress (EPC6)
Place of Presentation
Hotel Novotel London West, London (UK)
Year and Date
2015-08-25
Related Report
Int'l Joint Research
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[Presentation] 藻類の雌雄非対称性の成立2014
Author(s)
河野重行
Organizer
ゲノム支援公開シンポジウム
Place of Presentation
東京国際フォーラム(東京都、千代田区)
Year and Date
2014-12-12
Related Report
Invited
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[Presentation] 藻類バイオと電顕3D2014
Author(s)
大田修平、河野重行
Organizer
日本植物学会第78回大会
Place of Presentation
明治大学 生田キャンパス(神奈川県、川崎市)
Year and Date
2014-09-13
Related Report
Invited
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[Presentation] Phenotypic spectrum and 3D/TEM analysis of Parachlorella kessleri mutants produced by heavy-ion irradiation.2014
Author(s)
Ota, S., Takeshita, T., Yamazaki, T., Vítová, M., Zachleder, V., Abe, T., Kawano, S.
Organizer
BioTech2014 and 6th Czech-Swiss Symposium
Place of Presentation
Prague (Czech Republic)
Year and Date
2014-06-11 – 2014-06-14
Related Report
Invited
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[Presentation] Starch and lipid accumulation in Chlorella species under high light intensity in the laboratory and outdoor mass cultures.2014
Author(s)
Takeshita, T., Ivanov, V., Ota, S., Yamazaki, T., Zachleder, V., Kawano, S.
Organizer
BioTech2014 and 6th Czech-Swiss Symposium
Place of Presentation
Prague (Czech Republic)
Year and Date
2014-06-11 – 2014-06-14
Related Report
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[Book] Atlas of Plant cell Structure2014
Author(s)
Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hyashi, Y., (eds)
Total Pages
1- 202
Publisher
Springer
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