| Budget Amount *help |
¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Outline of Final Research Achievements |
In this project, we aimed to explore the evolutionary process of how fins have evolved into limbs during evolution of vertebrates. Studies of catsharks revealed that there was a shift in the balance of anterior and posterior fileds in their fin buds compared to that in mouse limb buds. Furthermore, catshark genome lacked a sequence found in mice and other tetrapods, which is responsible for preventing Gli3 expression in the posterior part of tetrapod limb buds. When we deliberately “posteriorised” pectoral fin buds of catshark, the fins lost anterior skeletal elements, and showed a single bone connected to the pectoral girdle as seen in fossil Tiktaalik pectoral fins. We also explored the developmental mechanisms behind the alteration of the neuronal framework during morphological diversification of limbs and found that Sema3A-dependent guidance system plays a key role in this process.
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