Understanding the mechanism of cellular differentiation and its usage for the regulation of morphological traits in rice

• Project/Area Number: 25292004
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Nagoya University
• Principal Investigator: Sato Yutaka 名古屋大学, 生命農学研究科, 准教授 (40345872)
• Co-Investigator(Kenkyū-buntansha): NAGASAWA Nobuhiro 秋田県立大学, 生物資源学部, 准教授 (90599268)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: イネ / 胚 / 分化 / 胚形成 / 胚発生 / 球状型胚発生突然変異体

Outline of Final Research Achievements

The goal of this research is to understand the molecular mechanism of cellular differentiation at the early stages of rice embryogenesis. We already demonstrated that, during the early stage of rice embryogenesis,
a receptor like kinase has pivotal function in cellular differentiation by using rice embryogenesis defective mutants (odm mutants). This suggests that a mobile signal could function in the process of cellular differentiation at the early stage of rice embryogenesis, because receptor like kinases are generally are important components of inter cellular signaling. In order to elucidate the entire components of the signaling module that operates at the cellular differentiation at the early stage of rice embryogenesis, we made double mutants with previously identified odm mutants. In addition, we screened and analyzed the suppressor mutations that restore odm phenotype.

Research Products

• [Journal Article] Genome-wide prediction of spatio-temporal gene expression patterns during early embryogenesis in rice. (2016)
  • Author(s): Jun-ichi Itoh, Yutaka Sato, Yutaka Sato, Ken-ichiro Hibara, Sae Shimizu-Sato, Hiromi Kobayashi, Hinako Takehisa, Karen Sanguinet, Nobukazu Namiki, Yoshiaki Nagamura
  • Journal Title: Development Volume: 143 Pages: 1217-1227
  • DOI: 10.1242/dev.123661
  • Peer Reviewed
• [Journal Article] Conserved functions of the MATE transporter BIG EMBRYO 1 in regulation of lateral organ size and initiation rate. (2015)
  • Author(s): Masaharu Suzuki, Yutaka Sato, Shan Wu, Byung-Ho Kang, Donald R. McCartly
  • Journal Title: Plant Cell Volume: 27 Issue: 8 Pages: 2288-2300
  • DOI: 10.1105/tpc.15.00290
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Rice SNF2 family helicase ENL1 is essential for syncytial endosperm development. (2015)
  • Author(s): Tomomi Hara, Hirokazu Katoh, Daisuke Ogawa, Yasuaki Kagawa, Yutaka Sato, Hidemi Kitano, Yasuo Nagato, Ryo Ishikawa, Akemi Ono, Tetsu Kinoshita, Shin Takeda, Tsukaho Hattori
  • Journal Title: The Plant Journal Volume: 81 Issue: 1 Pages: 1-12
  • DOI: 10.1111/tpj.12705
  • Peer Reviewed / Open Access
• [Journal Article] Expression of the rice microRNA <i>miR820</i> is associated with epigenetic modifications at its own locus (2013)
  • Author(s): Misuzu Nosaka, Akemi Ono, Aiko Ishiwata, Sae Shimizu-Sato, Kiyoe Ishimoto, Yusaku Noda and Yutaka Sato
  • Journal Title: Genes & Genetic Systems Volume: 88 Issue: 2 Pages: 105-112
  • DOI: 10.1266/ggs.88.105
  • NAID: 10031183176
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed
• [Journal Article] The copy number of rice CACTA DNA transposon carrying MIR820 does not correlate with MIR820 expression. (2013)
  • Author(s): Misuzu Nosaka, Aiko Ishiwata, Sae-Shimizu Sato, Akemi Ono, Kiyoe Ishimoto, Yusaku Noda, Yutaka Sato
  • Journal Title: Plant Signal. Behav. Volume: 8 Issue: 8 Pages: e25169-e25169
  • DOI: 10.4161/psb.25169
  • Peer Reviewed
• [Presentation] イネ球状型胚発生突然変異体 odm-192 の解析 (2016)
  • Author(s): 石本聖絵、佐藤豊
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] Rice MAPK6 regulates organ differentiation during embryogenesis through determination of regional identity. (2015)
  • Author(s): Kiyoe Ishimoto, Yutaka Sato
  • Organizer: International ERATO hHigashiyama Live-Holonics Symposium 2015
  • Place of Presentation: 名古屋大学
  • Year and Date: 2015-08-27
  • Int'l Joint Research
• [Presentation] A rice biotin auxotroph mutants reveal a function of water soluble vitamin, biotin, in organogenesis during embryogenesis. (2015)
  • Author(s): Ai Sugiki, Yutaka Sato
  • Organizer: International ERATO hHigashiyama Live-Holonics Symposium 2015
  • Place of Presentation: 名古屋大学
  • Year and Date: 2015-08-27
  • Int'l Joint Research