| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
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| Outline of Final Research Achievements |
Two cultivars (Capsicum annuum), from which RNase activities were not detected. So, we produced F1 hybrid and BC1 lines using the non-RNase cultivars and a cultivar which has very strong RNase activities (C. chinense ‘CP’). The RNase activity analysis using electrophoresis revealed that the gene controlling RNase activities would be single locus. Next, we tried to determine the causal protein of RNase activities using purified proteins, however we could not determine. The best way to determine the causal gene was considered to be positional cloning technique using the BC1 lines.
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