Intracellular osmotic homeostasis on cation membrane transporters

• Project/Area Number: 25292055
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Tohoku University
• Principal Investigator: Uozumi Nobuyuki 東北大学, 工学(系)研究科(研究院), 教授 (40223515)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000); Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 浸透圧 / 耐塩性 / イオン / 輸送体 / シロイヌナズナ / 脂質修飾 / 環境ストレス / イオンチャネル / イオン環境 / 機能調節

Outline of Final Research Achievements

Control of the Intracellular osmolarity of the plant and microorganism is necessary for adaptation of environmental change. The membrane transport of K and Na is indispensable to absorption of nutrients and hormone, circulation, maintenance of membrane potential, and a photosynthesis, which supported plant growth. We have elucidated the control mechanism of the ion translocator which plays the central role of the system to control homeostasis maintenance of K/Na balance in plant cells, the manifested analysis and regulation of the channels, and the molecular construction of mechanosensitive channel in cyanobacteria.

Research Products

• [Int'l Joint Research] Padova University(Italy)
• [Int'l Joint Research] Universwtiy of Munster(Germany)
• [Journal Article] HKT transporters mediate salt stress resistance in plants: from structure and function to the field. (2015)
  • Author(s): Hamamoto, S., Horie, T., Hauser, F., Deinlein, U., Schroeder J.I. and Uozumi, N.
  • Journal Title: Current Opinion in Biotechnology Volume: 32 Pages: 113-120
  • DOI: 10.1016/j.copbio.2014.11.025
  • Peer Reviewed / Open Access
• [Journal Article] The jasmonate-responsive GTR1 transporter is required for gibberellin-mediated stamen development in Arabidopsis (2015)
  • Author(s): H. Saito, T. Oikawa, S. Hamamoto, Y. Ishimaru, T. Utsumi, J. Chen, M. Kanamori, Y. Sasaki-Sekimoto, M. Shimojima, S. Masuda, Y. Kamiya, M. Seo, N. Uozumi, M. Ueda, H. Ohta, A
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 6095-6095
  • DOI: 10.1038/ncomms7095
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Defining membrane spanning domains and crucial membrane-localized acidic amino acid residues for K + transport of a Kup/HAK/KT-type Escherichia coli potassium transporter. (2014)
  • Author(s): Sato, Y., Nanatani, K., Hamamoto, S., Shimizu, M., Takahashi, M., Tabuchi-Kobayashi, M., Mizutani, A., Schroeder, J.I.,Souma, S, and Uozumi, N.
  • Journal Title: The Journal of Biochemistry Volume: 掲載確定 Issue: 5 Pages: 315-323
  • DOI: 10.1093/jb/mvu007
  • NAID: 40020056344
  • Peer Reviewed
• [Journal Article] Organelle-localized potassium transport system in plants (2014)
  • Author(s): Hamamoto, S., and Uozumi, N.
  • Journal Title: J. Plant Physiol. Volume: 171 Issue: 9 Pages: 737-747
  • DOI: 10.1016/j.jplph.2013.09.022
  • Peer Reviewed
• [Journal Article] Molecular cloning and expression analysis of a gene encoding KUP/HAK/KT-type potassium uptake transporter from Cryptomeria japonica. (2014)
  • Author(s): Hosoo, Y., Kimura, Y., Nanatani, K., and Uozumi, N.
  • Journal Title: Trees Volume: 28 Issue: 5 Pages: 1527-1537
  • DOI: 10.1007/s00468-014-1059-1
  • NAID: 120007106223
  • Peer Reviewed
• [Journal Article] The phosphoinositide PI(3,5)P2 mediates activation of mammalian but not plant TPC proteins: functional expression of endolysosomal channels in yeast and plant cells. (2014)
  • Author(s): Boccaccio, A., Scholz-Starke, J., Hamamoto, S., Larisch, N., Festa, M., Gutla, P. V. K., Costa, A., Dietrich, P., Uozumi, N., and Carpaneto, A.
  • Journal Title: Cell. Mol. Life Sci. Volume: 71 Issue: 21 Pages: 4275-4283
  • DOI: 10.1007/s00018-014-1623-2
  • Peer Reviewed
• [Journal Article] Sodium transport system in plant cells (2013)
  • Author(s): Toshio Yamaguchi, Shin Hamamoto, Nobuyuki Uozumi
  • Journal Title: Frontier in Plant Science Volume: 410 Pages: 1-7
  • DOI: 10.3389/fpls.2013.00410
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation in Synechocystis sp. PCC 6803 (2013)
  • Author(s): Nanatani K, Shijuku T, Akai M, Yukutake Y, Yasui M, Onai K, Morishita M, Ishiura M, Uozumi N
  • Journal Title: Channels Volume: 7 Issue: 4 Pages: 238-242
  • DOI: 10.4161/chan.25350
  • Peer Reviewed
• [Presentation] Lipidation of Arabidopsis CPK6 promotes the plasma membrane targeting and the stomata closure mediated by (2016)
  • Author(s): Saito, S., Hamamoto, S., Sato, Y., Uozumi, N., Hashimoto, K., Kudla, J., Utsumi, T., Moriya, K., Tozawa, Y., and Yamauchi, S.
  • Organizer: International workshop on Plant Membrane Biology
  • Place of Presentation: Annapolis, USA
  • Year and Date: 2016-06-05
  • Int'l Joint Research
• [Presentation] シロイヌナズナCPKの脂質修飾による細胞内局在調節および輸送体活性調節の解析 (2016)
  • Author(s): 齋藤俊也, 浜本晋, 内海俊彦, 守屋康子, 松浦愛子, 佐藤陽子, 野口寛人,戸澤譲, 山内清司, 橋本研志, Jörg Kudla, 魚住信之
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岩手大学 盛岡市
  • Year and Date: 2016-03-18
• [Presentation] シロイヌナズナCBL5の発現解析と脂質修飾による細胞内局在調節機構の解析 (2016)
  • Author(s): 竹林昂亮, 齋藤俊也, 内海俊彦, 守屋康子, 加藤恵, 佐藤陽子, 武藤潤,越智直樹, 橋本研志, Katrin Held, Jörg Kudla、上田実
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岩手大学 盛岡市
  • Year and Date: 2016-03-18
• [Presentation] シロイヌナズナNa輸送体AtHKT1の花器官の形成への関与 (2015)
  • Author(s): 浜本晋、平林一輝、加藤恵，堀江智明、Schroeder, J.I., Blumwald E., 横山隆亮、西谷和彦、石川敦司，魚住信之
  • Organizer: 第56回日本植物生理学会大会
  • Place of Presentation: 東京農業大学(東京)
  • Year and Date: 2015-03-20
• [Presentation] Ca2+結合タンパク質の脂質修飾による細胞膜への移行 (2014)
  • Author(s): 齋藤俊也, 武藤潤, 小林真由美, 佐藤陽子, 浜本晋, 魚住信之
  • Organizer: 化学系学協会東北大会
  • Place of Presentation: 山形大学(米沢市)
  • Year and Date: 2014-09-20
• [Presentation] シロイヌナズナCPKの脂質修飾による陰植物の気孔開閉に関与するイオン輸送体とその活性調節の解析制御因子に関する研究 (2014)
  • Author(s): 齋藤俊也、浜本晋、武藤潤，魚住信之
  • Organizer: 第55回日本植物生理学会大会
  • Place of Presentation: 富山大学(富山市)
  • Year and Date: 2014-03-18 – 2014-03-20
• [Presentation] 植物生存を支えるカリウム輸送 (2014)
  • Author(s): 魚住信之、浜本晋、七谷圭
  • Organizer: 第55回日本植物生理学会大会シンポジウム
  • Place of Presentation: 富山大学(富山市)
  • Year and Date: 2014-03-18 – 2014-03-20
  • Invited
• [Presentation] 環境適応の根底で機能するイオン輸送 (2013)
  • Author(s): 魚住信之
  • Organizer: DNA講演会
  • Place of Presentation: 名城大学(名古屋市)
  • Year and Date: 2013-11-09
  • Invited