• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Selection and functionality of candidate strains for probiotics in terms of co-evolution with host livestock

Research Project

Project/Area Number 25292165
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Animal production science
Research InstitutionKyoto Prefectural University

Principal Investigator

Ushida Kazunari  京都府立大学, 生命環境科学研究科(系), 教授 (50183017)

Co-Investigator(Kenkyū-buntansha) 大熊 盛也  国立研究開発法人理化学研究所, その他部局等, その他 (10270597)
丸山 史人  京都大学, 医学(系)研究科(研究院), 准教授 (30423122)
Co-Investigator(Renkei-kenkyūsha) TSUKAHARA Takamitsu  京都府立大学, 大学院生命環境科学研究科, 特任講師 (90562091)
INOUE Ryo  京都府立大学, 大学院生命環境科学研究科, 講師 (70443926)
Research Collaborator TSUCHIDA Sayaka  京都府立大学, 大学院生命環境科学研究科, 特任講師 (40734687)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Keywords共進化 / 腸内細菌 / ブタ / イノシシ
Outline of Final Research Achievements

Probiotics for livestock become popular, but most of products were derived from human-oriented products without verification in livestock. We isolated intestinal bacteria, which co-evolved with host pig during evolution of Suidae.We collected fresh feces from various animals of Suidae: domestic pigs (Japan and Africa), wild boar in Japan in wild or captivity, Red-river hogs and Worthogs in wild or captivity. NGS-metagenome was done to identify dietary- and host-effect. Bifidobacterium thermascidophilum and Lactobacillus mucosae were identified as Suidae-specific lactic acid bacteria and L. amylovorus is one became predominant with cereal feeding. Among co-evolved lactic acid bacteria, B. thermacidophilum often acquired tetracycline resistance, suggesting inappropriateness of Bifidobacterium as probiotics in pigs. L. mucosae is a better candidate of probiotics for pigs; one strain showed anti-pathogenic activity.

Report

(4 results)
  • 2016 Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (11 results)

All 2016 2015 2014 Other

All Int'l Joint Research (3 results) Journal Article (2 results) (of which Acknowledgement Compliant: 2 results,  Peer Reviewed: 1 results) Presentation (5 results) (of which Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] IRET(ガボン)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of Ghana(ガーナ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Makerere University(ウガンダ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 野生動物の腸内細菌研究の意義と展望2016

    • Author(s)
      牛田一成・土田さやか
    • Journal Title

      ルーメン研究会報

      Volume: 27 Pages: 23-29

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] 様々な動物から分離される乳酸菌・ビフィズス菌 ~ゴリラにはゴリラの乳酸菌? ヒトにはヒトの乳酸2016

    • Author(s)
      土田さやか/牛田一成
    • Journal Title

      日本乳酸菌学会誌

      Volume: 27 Pages: 25-33

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] イノシシとブタに共通するビフィズス菌のゲノム解析と薬剤耐性遺伝子の偏在について2016

    • Author(s)
      牛田一成 土田さやか 丸山史人 大熊盛也
    • Organizer
      日本畜産学会第121回大会
    • Place of Presentation
      日本獣医生命科学大学
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 様々な動物から分離される乳酸菌・ビフィズス菌2015

    • Author(s)
      牛田一成
    • Organizer
      日本乳酸菌学会
    • Place of Presentation
      昭和女子大学
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Domestication developed the pig-type intestinal microbiota2015

    • Author(s)
      Kazunari Ushida, Sayaka Tsuchida, Fumito Maruyama
    • Organizer
      International symposium on Genome Science 2015
    • Place of Presentation
      一橋ホール
    • Year and Date
      2015-01-20 – 2015-01-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 宿主の家畜化が腸内細菌にもたらすインパクト-乳酸桿菌のゲノム解析で見えてきたもの2014

    • Author(s)
      牛田一成 土田さやか
    • Organizer
      ルーメン研究会
    • Place of Presentation
      明治大学
    • Year and Date
      2014-09-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 宿主との共進化をメルクマールとした家畜用プロバイオティクスの選抜と機能開発2014

    • Author(s)
      牛田一成 土田さやか 丸山史人 大熊盛也
    • Organizer
      ゲノム支援拡大班会議
    • Place of Presentation
      神戸ポートピアホテル
    • Year and Date
      2014-08-20
    • Related Report
      2014 Annual Research Report
  • [Remarks] ほ乳類消化管における微生物生理と生態-宿主と腸内細菌の共進化

    • URL

      http://seika.kpu.ac.jp/~k_ushida/ushidaintro.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2022-02-01  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi