Selection and functionality of candidate strains for probiotics in terms of co-evolution with host livestock
| Project/Area Number |
25292165
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
Ushida Kazunari 京都府立大学, 生命環境科学研究科(系), 教授 (50183017)
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| Co-Investigator(Kenkyū-buntansha) |
大熊 盛也 国立研究開発法人理化学研究所, その他部局等, その他 (10270597)
丸山 史人 京都大学, 医学(系)研究科(研究院), 准教授 (30423122)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUKAHARA Takamitsu 京都府立大学, 大学院生命環境科学研究科, 特任講師 (90562091)
INOUE Ryo 京都府立大学, 大学院生命環境科学研究科, 講師 (70443926)
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| Research Collaborator |
TSUCHIDA Sayaka 京都府立大学, 大学院生命環境科学研究科, 特任講師 (40734687)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | 共進化 / 腸内細菌 / ブタ / イノシシ |
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| Outline of Final Research Achievements |
Probiotics for livestock become popular, but most of products were derived from human-oriented products without verification in livestock. We isolated intestinal bacteria, which co-evolved with host pig during evolution of Suidae.We collected fresh feces from various animals of Suidae: domestic pigs (Japan and Africa), wild boar in Japan in wild or captivity, Red-river hogs and Worthogs in wild or captivity. NGS-metagenome was done to identify dietary- and host-effect. Bifidobacterium thermascidophilum and Lactobacillus mucosae were identified as Suidae-specific lactic acid bacteria and L. amylovorus is one became predominant with cereal feeding. Among co-evolved lactic acid bacteria, B. thermacidophilum often acquired tetracycline resistance, suggesting inappropriateness of Bifidobacterium as probiotics in pigs. L. mucosae is a better candidate of probiotics for pigs; one strain showed anti-pathogenic activity.
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Report
(4 results)
Research Products
(11 results)
