| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
We prepared an antibody that recognizes the schizont stage of small piroplasm to observe the schizont-infected cells, but such cells were not detected. However, we were able to 1) Sequence the whole genome of two genotypes small piroplasm, 2) Establish a “tick-SCID mouse” model for experimental infection and thereby analyze the gene expression profiles of sporozoite stage, 3) Identify the RBC receptors targeted by protozoan membrane proteins (p23 and MPSP), and 4) Investigate the epidemiology and genetic diversity of small piroplasm in Japan and several endemic countries. Resultantly, we have gained a more thorough understanding of the mechanisms involving the growth of bovine small piroplasm. Furthermore, we have comprehensively reviewed the ecology, epidemiology, genetic polymorphism, and evolution of the small piroplasm in an article that was published in an international scientific journal.
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