Development of therapeutic agents and vaccines against feline infectious peritonitis

• Project/Area Number: 25292183
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Kitasato University
• Principal Investigator: Hohdatsu Tsutomu 北里大学, 獣医学部, 教授 (00129264)
• Co-Investigator(Kenkyū-buntansha): Takano Tomomi 北里大学, 獣医学部, 准教授 (20525018)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000); Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 猫伝染性腹膜炎 / ワクチン / 抗ウイルス薬 / TNF-α / コロナウイルス / 分子標的薬 / 抗サイトカイン療法 / 抗TNF製剤

Outline of Final Research Achievements

Feline infectious peritonitis (FIP) is a virus-induced fatal disease in cats. There have been no reports on agents that have exhibited therapeutic or preventive effects. On the basis of these facts, we have tried to develop therapeutic agents and vaccines against FIP. We selected peptides that strongly induced Th1 activity from the peptides derived from FIPV structural proteins. Prevention against FIPV-induced FIP was slightly better in SPF cats treated with peptides derived from the structural protein of FIPV. We have prepared monoclonal antibodies (MAb) that neutralize feline TNF-α(MAb 2-4).Progression to FIP was prevented in 2 out of 3 cats treated with MAb 2-4. These results may provide important information for the development of therapeutic agents and vaccines against FIP.

Research Products

• [Journal Article] Differential effect of cholesterol on type I and II feline coronavirus infection (2016)
  • Author(s): Takano T, Satomi Y, Oyama Y, Doki T, Hohdatsu T.
  • Journal Title: Arch Virol Volume: 161 Issue: 1 Pages: 125-133
  • DOI: 10.1007/s00705-015-2655-0
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Therapeutic effect of anti-feline TNF-alpha monoclonal antibody for feline infectious peritonitis. (2016)
  • Author(s): Doki T, Takano T, Kawagoe K, Kito A, Hohdatsu T.
  • Journal Title: Res Vet Sci Volume: 104 Pages: 17-23
  • DOI: 10.1016/j.rvsc.2015.11.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Detection of canine astrovirus in dogs with diarrhea in Japan. (2015)
  • Author(s): Takano T, Takashina M, Doki T, Hohdatsu T.
  • Journal Title: Archives of Virology Volume: in press Pages: 13-20
  • DOI: 10.1016/j.virusres.2015.04.011
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Differential effects of viroporin inhibitors against feline infectious peritonitis virus serotypes I and II (2015)
  • Author(s): Takano T, Nakano K, Doki T, Hohdatsu T.
  • Journal Title: Arch Virol. Volume: 160(5) Pages: 1163-1170
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Screening and identification of T helper 1 and linear immunodominant antibody-binding epitopes in spike 1 domain and membrane protein of feline infectious peritonitis virus. (2014)
  • Author(s): Takano T., Morioka, H., Gomi, K., Tomizawa, K., Doki, T. and Hohdatsu, T.
  • Journal Title: Vaccine Volume: 32 Issue: 16 Pages: 1834-1840
  • DOI: 10.1016/j.vaccine.2014.01.074
  • Peer Reviewed
• [Journal Article] Evaluation of protective efficacy of the synthetic peptide vaccine containing the T helper 1 epitope with CpG oligodeoxynucleotide against feline infectious peritonitis virus infection in cats. (2014)
  • Author(s): Takano, T., Tomizawa, K., Morioka, H., Doki, T. and Hohdatsu, T.
  • Journal Title: Antivir Ther Volume: in press Issue: 7 Pages: 645-650
  • DOI: 10.3851/imp2735
  • Peer Reviewed
• [Journal Article] Generation, characterization and therapeutic potential of anti-feline TNF-alpha MAbs for feline infectious peritonitis. (2013)
  • Author(s): Doki, T., Takano, T., Nishiyama, Y., Nakamura, M. and Hohdatsu, T.
  • Journal Title: Res Vet Sci Volume: 95 Issue: 3 Pages: 1248-1254
  • DOI: 10.1016/j.rvsc.2013.09.005
  • Peer Reviewed
• [Journal Article] Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests. (2013)
  • Author(s): Takano, T., Ishihara, Y., Matsuoka, M., Yokota, S., Matsuoka-Kobayashi, Y., Doki, T. and Hohdatsu, T.
  • Journal Title: J Virol Methods Volume: 196 Pages: 1-6
  • DOI: 10.1016/j.jviromet.2013.10.014
  • Peer Reviewed
• [Presentation] ネコTNF-αを中和するマウスモノクローナル抗体のネコ伝染性腹膜炎(FIP)に対する治療効果とマウス・ネコキメラ化抗体の作製 (2015)
  • Author(s): 土岐朋義、川越浩平、高野友美、宝達勉
  • Organizer: 第11回日本獣医内科学アカデミー学術集会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2015-02-20 – 2015-02-22
• [Presentation] I型feline infectious peritonitis virus (FIPV) S蛋白質(S1ドメイン)由来peptideのcoronavirusに対する感染抑制効果 (2014)
  • Author(s): 土岐朋義、高野友美、小山祐介、宝達勉
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] I型猫伝染性腹膜炎ウイルス(FIPV)KU-2株N蛋白質由来peptide(NP)のFIP発症予防効果の検討 (2013)
  • Author(s): 土岐朋義、高野友美、冨沢圭佑、盛岡寛行、佐藤亮一、宝達勉
  • Organizer: 日本獣医学会
  • Place of Presentation: 岐阜大学(岐阜県岐阜市)
• [Presentation] 猫伝染性腹膜炎ウイルスの構造蛋白質(MおよびS1 domain)におけるTh1 epitopeの検索とTh1活性誘導能について (2013)
  • Author(s): 高野友美、盛岡寛行、冨沢圭佑、五味宏二、土岐朋義、宝達勉
  • Organizer: 日本獣医学会
  • Place of Presentation: 岐阜大学(岐阜県岐阜市)
• [Book] Coronaviruses (2015)
  • Author(s): Takano T, Hohdatsu T.
  • Total Pages: 283
  • Publisher: Humana Press
• [Remarks] 猫伝染性腹膜炎(FIP)に対するクロロキンの治療効果
  • URL: https://www.kitasato-u.ac.jp/vmas/faculty/vm/topix_index.html