• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies of physiological function of cauxin, a major urinary protein, by development of cauxin-knocking out cats

Research Project

Project/Area Number 25292191
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Integrative animal science
Research InstitutionIwate University

Principal Investigator

Yamashtia Tetsuro  岩手大学, 農学部, 教授 (20202377)

Co-Investigator(Kenkyū-buntansha) MIYAZAKI MASAO  岩手大学, 農学部, 准教授 (20392144)
KATAYAMA MASAAKI  岩手大学, 農学部, 准教授 (70436054)
SAWAI KEN  岩手大学, 農学部, 教授 (90390864)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords遺伝子改変動物 / 酵素 / 尿タンパク質
Outline of Final Research Achievements

The domestic cat excretes cauxin with carboxylesterase activity as a major urinary protein. Our previous studies demonstrated that cauxin hydrolyzes 3-methylbutanol-cysteinylglycine and produces glycine and felinine, a cat-specific amino acid. However little is known whether cauxin has functions other than peptidase in the urine. In this study, we aimed to produce cauxin-knocking our cats to examine the functions of cauxin in cats.
We used transcription activator-like effector nucleases (TALEN) that are restriction enzymes to cut specific sequences of cauxin exon 1 and 2. Our analysis indicated that TALENs targeting exon 2 could destroy the cauxin gene in cat kidney cultured cells. On the other hand, we also found that felinine metabolites are excreted into the urine of humans, mice, and dogs that excrete no cauxin. The results suggest that cauxin excretion is necessary to excrete free felinine that is decomposed to volatile compounds for scent communication.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (5 results)

All 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (2 results) (of which Invited: 1 results)

  • [Journal Article] Kite shield-shaped wedge recession for the surgical treatment of medial patellar luxation in seven small-breed dogs.2016

    • Author(s)
      Katayama, M., Ogaya, H., Shimamura, S., and Uzuka, Y.
    • Journal Title

      Vet. Surg.

      Volume: 45 Issue: 1 Pages: 66-70

    • DOI

      10.1111/vsu.12422

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationship of glomerular filtration rate based on serum iodixanol clearance to IRIS staging in cats with chronic kidney disease2015

    • Author(s)
      2.Iwama, R., Sato, T., Katayama, M., Shimamura, S., Satoh, H., Ichijo, T., and Furuhama, K.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 77 Issue: 8 Pages: 1033-1035

    • DOI

      10.1292/jvms.14-0494

    • NAID

      130005094667

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of downregulating GLIS1 transcript on preimplantation development and gene expression of bovine embryos2015

    • Author(s)
      3.Takahashi, K., Sakurai, N., Emura, N., Hashizume, T. and Sawai, K.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 61 Issue: 5 Pages: 369-374

    • DOI

      10.1262/jrd.2015-029

    • NAID

      130005104112

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] 過剰なコレステロール生合成を抑制する新規代謝経路の解明2015

    • Author(s)
      弗田彩心、北條渉、宮崎珠子、土谷和平、木村賢一、山下哲郎、宮崎雅雄
    • Organizer
      第38回分子生物学会年会・第88回生化学会大会合同大会
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 完全肉食動物への進化で獲得したネコ特有なケミカルシグナルの生合成機構2015

    • Author(s)
      宮崎雅雄
    • Organizer
      新学術領域研究「天然物ケミカルバイオロジー」地区ミニシンポジウム
    • Place of Presentation
      名古屋大学
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
    • Invited

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi