Novel regulatory mechanism for intracellular calcium ion concentration by membrane microdomain-localized glycans in sperm
| Project/Area Number |
25292216
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
Kitajima Ken 名古屋大学, 生物機能開発利用研究センター, 教授 (80192558)
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| Co-Investigator(Kenkyū-buntansha) |
SATO CHIHIRO 名古屋大学, 生物機能開発利用研究センター, 准教授 (10343211)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Yoshiki 独立行政法人理化学研究所, 糖鎖構造生物学研究チーム, チームリーダー (90323451)
SHINGYOJI Chikako 東京大学, 理学系研究科, 准教授 (80125997)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | 糖鎖 / 受精 / 精子 / 生理活性 / 蛋白質 / カルシウム調節 / 受精能獲得 / GPIアンカー |
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| Outline of Final Research Achievements |
Many studies using glycan-related probes have reported the importance of glycan chains in fertilization. However, glycan-related gene-modified studies have told that glycans are not always essential for a successful fertilization. It is thus a currently important subject to re-evaluate molecular mechanisms for glycan-involved events at fertilization. To solve the subject, we focused on the "membrane microdomain" that is molecular complex of proteins and lipids with highly concentrated glycans on plasma membrane. In this study, we demonstrated the followings: (1) Glycan-enriched GPI-anchor peptide on the sperm membrane microdomains ubiquitously occurs not only in sea urchin and pig, but also avians and amphibians that have never been shown to contain such GPI-anchored molecules before; (2) These glycans are involved in sperm motility through regulating the intracellular calcium ion concentration in sperm.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm2015
Author(s)
Estelle Garenaux, Mayumi Kanagawa, Tomoyuki Tsuchiyama, Kazuki Hori, Takeru Kanazawa, Ami Goshima, Mitsuru Chiba, Hiroshi Yasue, Akemi Ikeda, Yoshiki Yamaguchi, Chihiro Sato, Ken Kitajima
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Journal Title
Journal of Biological Chemistry
Volume: 9
Issue: 9
Pages: 5484-5501
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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