Mechanisms for the navigation of the intracellular transport
| Project/Area Number |
25293046
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Okada Yasushi 国立研究開発法人理化学研究所, 生命システム研究センター, チームリーダー (50272430)
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| Co-Investigator(Kenkyū-buntansha) |
Takai Akira 国立研究開発法人理化学研究所, 生命システム研究センター, 基礎科学特別研究員 (60637205)
Shima Tomohiro 独立行政法人理化学研究所, 生命システム研究センター, 基礎科学特別研究員 (60631786)
Ikeda Kazuho 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (20642565)
Ito Yoko 国立研究開発法人理化学研究所, 生命システム研究センター, 特別研究員 (60584571)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Keywords | 細胞内輸送 / 細胞極性 / 微小管 / 分子モーター / キネシン / 微小管結合タンパク質 |
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| Outline of Final Research Achievements |
The navigation mechanism for the intracellular transport has remained unclear. We are proposing a model that the conformational differences of micorubules between the GTP-bound and GDP-bound state would serve as the directional cue for kinesins that drive the intracellular transport into the neuronal axons. In this study, we have clarified the underlying mechanisms for this transport by the combination of single molecule imaging and the structural analyses.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle.2015
Author(s)
Chinen T, Liu P, Shioda S, Pagel J, Cerikan B, Lin TC, Gruss O, Hayashi Y, Takeno H, Shima T, Okada Y, Hayakawa I, Hayashi Y, Kigoshi H, Usui T, Schiebel E
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Journal Title
Nature Communications
Volume: 6
Issue: 1
Pages: 8722-8722
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Quantitative analysis of APP axonal transport in neurons: role of JIP1 in enhanced APP anterograde transport.2014
Author(s)
Chiba K, Araseki M, Nozawa K, Furukori K, Araki Y, Matsushima T, Nakaya T,Hata S, Saito Y, Uchida S, Okada Y, Nairn AC, Davis RJ, Yamamoto T, Kinjo M, Taru, H, Suzuki T.
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Journal Title
Mol Biol Cell
Volume: 25
Issue: 22
Pages: 3569-3580
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] A spontaneously blinking fluorophore based on intramolecular spirocyclization for live-cell super-resolution imaging2014
Author(s)
Shin-nosuke Uno, Mako Kamiya, Toshitada Yoshihara, Ko Sugawara, Kohki Okabe, Mehmet C. Tarhan, Hiroyuki Fujita, Takashi Funatsu, Yasushi Okada, Seiji Tobita, Yasuteru Urano
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Journal Title
Nature Chemistry
Volume: 6
Issue: 8
Pages: 681-689
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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