Analysis of the molecular mechanism and clinical targets for neural tumors via the loss of function of NF1/NF2 gene products

• Project/Area Number: 25293312
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Kumamoto University
• Principal Investigator: Araki Norie 熊本大学, 大学院生命科学研究部(医), 准教授 (80253722)
• Co-Investigator(Kenkyū-buntansha): KURATSU Junichi 熊本大学, 大学院生命科学研究部, 教授 (20145296) ; NAKAMURA Hideo 熊本大学, 付属病院, 講師 (30359963) ; 小林 大樹 熊本大学, 大学院生命科学研究部, 産学関連系研究員 (20448517)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: neurofibromatosis / NF1 / proteomics / mTOR / TCTP / 神経線維腫症 / NF2 / プロテオミクス / トランスクリプトーム / 融合プロテオミクス / 脳神経系腫瘍

Outline of Final Research Achievements

Neurofibromatosis type 1/2 (NF1/2) is an autosomal dominant disease that predisposes individuals to develop neural tumors including neurofibromas and malignant tumors etc. To identify novel biological targets for NF-associated tumors, a unique integrated-omics was performed, and a novel abnormal network, “Translationally controlled tumor protein (TCTP)-mTOR/EF signalings” was identified. This network activates the MAPK/PI3K-AKT-mTOR and specific EF1 complex associated translational signalings in NF1 tumors. In NF1-deficient MPNST cells, MAPK/PI3K/mTOR inhibitors downregulated TCTP associated cell expansions, and TCTP knockdown (or overexpression) suppressed (or activated) mTOR/EF1 signalings. Artesunate, a TCTP target, inhibited the TCTP-mTOR/EF1 signal cascade and suppressed the viability of MPNST cells significantly. These findings suggest that TCTP-mTOR/EF signaling is implicated in the progression of NF1-tumors and could serve as a biological target for the specific therapy.

Research Products

• [Journal Article] CXCL12/CXCR4 activation by cancer-associated fibroblasts promotes integrin β1 clustering and invasiveness in gastric cancer. (2016)
  • Author(s): Izumi D, Ishimoto T, Miyake K, Sugihara H, Eto K, Sawayama H, Yasuda T, Kiyozumi Y, Kaida T, Kurashige J, Imamura Y, Hiyoshi Y, Iwatsuki M, Iwagami S, Baba Y, Sakamoto Y, Miyamoto Y, Yoshida N, Watanabe M, Takamori H, Araki N, Tan P, Baba H.
  • Journal Title: Int J Cancer Volume: 1;138(5) Issue: 5 Pages: 1207-19
  • DOI: 10.1002/ijc.29864
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of a fully automated two dimensional electrophoresis device, Auto-2D, and its clinical applications. Proteome Letters, 2016 in press (2016)
  • Author(s): Araki N, Goto S, Matsunaga T, Kinoshita H, Nishimura M, Midorikawa U, Kobayashi D, Yabe K.
  • Journal Title: Proteome Letters Volume: 印刷中
  • Open Access
• [Journal Article] プロテオーム解析を基盤とした融合的オミクス解析による脳神経系腫瘍の解析～神経線維腫症の解析から学ぶ (2016)
  • Author(s): 荒木令江, 南部（新堀）晶子, 小林大樹
  • Journal Title: 医学のあゆみ Volume: 印刷中
• [Journal Article] A case of an epithelioid glioblastoma with the BRAF V600E mutation colocalized with BRAF intact low-grade diffuse astrocytoma. (2016)
  • Author(s): Kuroda JI, Nobusawa S, Nakamura H, Yokoo H, Ueda R, Makino K, Yano S, Kuratsu JI.
  • Journal Title: Neuropathology Volume: 36 Issue: 2 Pages: 181-186
  • DOI: 10.1111/neup.12258
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina. (2015)
  • Author(s): Tokuda K, Kuramitsu Y, Byron B, Kitagawa T, Tokuda N, Kobayashi D, Nagayama M, Araki N, Sonoda K , Nakamura K.
  • Journal Title: Biochem Biophys Res Commun Volume: 463 Issue: 4 Pages: 593-599
  • DOI: 10.1016/j.bbrc.2015.05.102
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quality of Life and Clinical Features of Long-Term Survivors Surgically Treated for Pediatric Craniopharyngioma (2015)
  • Author(s): Yano S, Kudo M, Hide T, Shinojima N, Makino K, Nakamura H, Kuratsu JI
  • Journal Title: World neurosurgery Volume: 85 Pages: 153-62
  • DOI: 10.1016/j.wneu.2015.08.059
  • Peer Reviewed / Open Access
• [Journal Article] Brain tumor stem cell (2015)
  • Author(s): Hide T. Makino K. Nakamura H, Kuratsu J
  • Journal Title: Nihon Rinsho Volume: 73 Pages: 836-843
  • NAID: 40020445324
• [Journal Article] TogoTable: cross-database annotation system using the Resource Description Framework (RDF) data model. (2014)
  • Author(s): Kawano S, Watanabe T, Mizuguchi S, Araki N, Katayama T and Yamaguchi A.
  • Journal Title: Nucleic Acids Resarch Volume: 42 Issue: W1 Pages: W442-W448
  • DOI: 10.1093/nar/gku403
  • Peer Reviewed / Open Access
• [Journal Article] Translationally Controlled Tumor Protein is a Novel Biological Target for Neurofibromatosis Type 1 (NF1)-associated Tumors. (2014)
  • Author(s): Kobayashi, D., Hirayama M., Komohara, Y., Mizuguchi, S, Wilson Morifuji, M. Patrakitkomjorn, S, Ihn, H., Takeya, M, Kuramochi, A., and Araki, N*.
  • Journal Title: J. Biol. Chem. Volume: 289(38) Issue: 38 Pages: 26314-26
  • DOI: 10.1074/jbc.m114.568253
  • Peer Reviewed / Open Access
• [Journal Article] Development of a fully automated two-dimensional electrophoresis device, Auto-2D, and its application for the integrated proteomics (2014)
  • Author(s): 荒木令江*, 南部（新堀 晶子）晶子, 西村宗総、緑川 宇一, 小林大樹
  • Journal Title: 生物物理化学 Volume: 58(2) Pages: 39-42
  • NAID: 130004704682
  • Peer Reviewed
• [Journal Article] 融合プロテオミクスによるは発がんメカニズムの解析 (2014)
  • Author(s): 荒木令江*, 南部（新堀 晶子）晶子, 小林大樹
  • Journal Title: 医学のあゆみ Volume: 251(10) Pages: 939-947
• [Journal Article] Clinical characteristics and pathogenesis of cerebellar glioblastoma. (2014)
  • Author(s): 4.Takahashi Y, Makino K, Nakamura H, Hide T, Yano S, Kamada H, Kuratsu JI.
  • Journal Title: ol Med Rep. Volume: 10 Issue: 5 Pages: 2383-2388
  • DOI: 10.3892/mmr.2014.2549
  • Peer Reviewed / Open Access
• [Journal Article] Characteristics of brain metastases from esophageal carcinoma. (2014)
  • Author(s): Yamamoto T, Kuroda J, Takezaki T, Shinojima N, Hide T, Makino K, Nakamura H, Yano S, Nishi T, Kuratsu J
  • Journal Title: Surg Neurol Int. Volume: 22 Issue: 1 Pages: 137-137
  • DOI: 10.4103/2152-7806.141468
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A patient with medulloblastoma in its early developmental stage. (2014)
  • Author(s): Shinojima N, Nakamura H, Tasaki M, Kameno K, Anai S, Iyama K, Ando Y, Seto H, Kuratsu J.
  • Journal Title: J Neurosurg Pediatr Volume: 14(6) Issue: 6 Pages: 615-20
  • DOI: 10.3171/2014.8.peds13590
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Integrated proteomics identified novel activation of dynein IC2-GR-COX-1 signaling in NF1 disease model cells. (2013)
  • Author(s): Hirayama M, Kobayashi D, Mizuguchi S, Morikawa T, Nagayama M, Midorikawa U, Wilson MM, Nambu AN, Yoshizawa AC, Kawano S, Araki N.
  • Journal Title: Molecular & Cellular Proteomics Volume: 28 Issue: 5 Pages: 1377-94
  • DOI: 10.1074/mcp.m112.024802
  • Peer Reviewed
• [Journal Article] Glioma Initiating Cells Form a Differentiation Niche Via the Induction of Extracellular Matrices and Integrin aV (2013)
  • Author(s): Akiko Niibori-Nambu, Uichi Midorikawa, Souhei Mizuguchi, Takuichiro Hide, Minako Nagai,Yoshihiro Komohara, Megumi Nagayama, Mio Hirayama, Daiki Kobayashi, Nobuyuki Tsubota,Tatsuya Takezaki, Keishi Makino, Hideo Nakamura, Motohiro Takeya, Junichi Kuratsu, Norie Araki
  • Journal Title: PLoS ONE Volume: 8(5) Issue: 5 Pages: e59558-e59558
  • DOI: 10.1371/journal.pone.0059558
  • Peer Reviewed
• [Journal Article] Enhancement of human cancer cell motility and invasiveness by anaphylatoxin C5a via aberrantly expressed C5a-receptor (CD88). (2013)
  • Author(s): Nitta, H. et al.
  • Journal Title: Clinical Cancer Reseach Volume: 19 Issue: 8 Pages: 2004-2013
  • DOI: 10.1158/1078-0432.ccr-12-1204
  • Peer Reviewed
• [Journal Article] CA-S27 : a novel Lewis a associated carbohydrate epitope is diagnostic and prognostic for cholangiocarcinoma (2013)
  • Author(s): Silsirivanit A, Araki N, Wongkham C, Vaeteewoottacharn K, Pairojkul C, Kuwahara K, Narimatsu Y, Sawaki H, Narimatsu H, Okada S, Sakaguchi N, Wongkham S
  • Journal Title: Cancer Sci Volume: 104 Issue: 10 Pages: 1278-1284
  • DOI: 10.1111/cas.12222
  • Peer Reviewed
• [Journal Article] Integrated view of the human chromosome X-centric proteome project. (2013)
  • Author(s): Yamamoto T, Nakayama K, Hirano H, Tomonaga T, Ishihama Y, Yamada T, Kondo T,Kodera Y, Sato Y, Araki N, Mamitsuka H, Goshima N.
  • Journal Title: J Proteome Res. Volume: 12 Issue: 1 Pages: 58-61
  • DOI: 10.1021/pr300844p
  • Peer Reviewed
• [Journal Article] The complete amino acid sequence, and enzymatic properties of an i-type lysozyme isolated from the common orient clam (Meretrix lusoria) (2013)
  • Author(s): Kuwano Y, Yoneda K, Kawaguchi Y, Araki N, Araki T.
  • Journal Title: Bioscience Biotechnology, and Biochemistry Volume: 77(11) Pages: 2269-77
  • NAID: 130003381938
  • Peer Reviewed
• [Journal Article] Epiplakin modifies the motility of the HeLa cells and accumulates at the outer surfaces of 3-D cell clusters. (2013)
  • Author(s): Shimada H, Nambu-Niibori A, Wilson-Morifuji M, Mizuguchi S, Araki N, Sumiyoshi H, Sato M, Mezaki Y, Senoo H, Ishikawa K, Hatano Y, Okamoto O, Fujiwara S
  • Journal Title: J Dermatol Volume: 40 Issue: 4 Pages: 249-58
  • DOI: 10.1111/1346-8138.12076
  • Peer Reviewed
• [Journal Article] Renal denervation prevents stroke and brain injury via attenuation of oxidative stress in hypertensive rats (2013)
  • Author(s): Nakagawa T, Hasegawa Y, Uekawa K, Ma M, Katayama T, Sueta D, Toyama K, Kataoka K, Koibuchi N, Maeda M, Kuratsu J, Kim-Mitsuyama S
  • Journal Title: J Am Heart Assoc Volume: 2(5) Issue: 5
  • DOI: 10.1161/jaha.113.000375
  • Peer Reviewed
• [Journal Article] Skull Metastasis From Intrahepatic Cholangiocarcinoma: Report of 3 Cases and Review of the Literature. (2013)
  • Author(s): Fujimoto K, Kuroda JI, Makino K, Hasegawa Y, Kuratsu JI
  • Journal Title: Neurol Med Chir Volume: 53(10) Pages: 717-21
  • Peer Reviewed
• [Journal Article] Seizures as a presenting symptom in neurosurgical patients: A retrospective single-institution analysis. (2013)
  • Author(s): Hamasaki T, Yamada K, Kuratsu JI.
  • Journal Title: Clin Neurol Neurosurg. Volume: 115(11) Issue: 11 Pages: 2336-40
  • DOI: 10.1016/j.clineuro.2013.08.016
  • Peer Reviewed
• [Journal Article] Discovery of an uncovered region in fibrin clots and its clinical significance. (2013)
  • Author(s): Hisada Y, Yasunaga M, Hanaoka S, Saijou S, Sugino T, Tsuji A, Saga T, Tsumoto K, Manabe S, Kuroda J, Kuratsu J, Matsumura Y.
  • Journal Title: Sci Rep. Volume: 3 Issue: 1 Pages: 2604-2604
  • DOI: 10.1038/srep02604
  • Peer Reviewed
• [Journal Article] Usefulness of the coherence entrainment test for deep brain stimulation for a patient with atypical tremor. (2013)
  • Author(s): Hasegawa Y, Yamada K, Uekawa K, Hamasaki T, Fujise N, Kuratsu J.
  • Journal Title: J Clin Neurosci Volume: 20(8) Pages: 1161-2
  • Peer Reviewed
• [Journal Article] Pretreatment with rosuvastatin protects against focal cerebral ischemia/reperfusion injury in rats through attenuation of oxidative stress and inflammation. (2013)
  • Author(s): Ma M, Uekawa K, Hasegawa Y, Nakagawa T, Katayama T, Sueta D, Toyama K, Kataoka K, Koibuchi N, Kuratsu J, Kim-Mitsuyama S.
  • Journal Title: Brain Res. Volume: 1519 Pages: 87-94
  • DOI: 10.1016/j.brainres.2013.04.040
  • Peer Reviewed
• [Journal Article] A case of ecchordosis physaliphora presenting with an abducens nerve palsy: A rare symptomatic case managed with endoscopic endonasal transsphenoidal surgery (2013)
  • Author(s): Yamamoto T, Yano S, Hide T, Kuratsu J.
  • Journal Title: Surg Neurol Int. Volume: - Issue: 1 Pages: 13-13
  • DOI: 10.4103/2152-7806.106562
  • Peer Reviewed
• [Journal Article] A 6-fr guiding catheter (slim guide(®)) for use with multiple microdevices. An experimental study (2013)
  • Author(s): Kai Y, Ohmori Y, Watanabe M, Kaku Y, Morioka M, Hirano T, Yano S, Kawano T, Hamada JI, Kuratsu JI.
  • Journal Title: Interv Neuroradiol. Volume: 19(1) Pages: 7-15
  • Peer Reviewed
• [Journal Article] TGF-β mediates homing of bone marrow -derived human mesenchymal stem cells to glioma stem cells (2013)
  • Author(s): Naoki Shinojima, Tatsuya Takezaki, Jun-ichi Kuratsu(他8 名)
  • Journal Title: Cancer Research Volume: 73(7) Issue: 7 Pages: 2333-2344
  • DOI: 10.1158/0008-5472.can-12-3086
  • Peer Reviewed
• [Presentation] プロテオミクスを基盤とした統合オミクスによるがん組織細胞の異常シグナルネットワークの抽出と検証 (2015)
  • Author(s): 荒木 令江 、南部-新堀 晶子 、シルシリバニト アチト 、小林 大樹
  • Organizer: BMB2015 (第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸）
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] POST: プロテオーム解析ワークフローの標準化 (2015)
  • Author(s): 守屋 勇樹、河野 信、奥田 修二郎、山本 格、松本 雅記、小林 大樹、荒木 令江、吉沢 明康、五斗 進、田畑 剛、杉山 直幸、石濱 泰
  • Organizer: BMB2015 (第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸）
  • Year and Date: 2015-12-01
• [Presentation] Interactome analysis identified a novel interaction form of TCTP and translation elongation factors in Neurofibromatosis-type 1-associated tumors. (2015)
  • Author(s): 小林 大樹 、徳田 高穂 、長山 慈 、平山 未央 、大槻 純男 、荒木 令江
  • Organizer: BMB2015 (第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸）
  • Year and Date: 2015-12-01
• [Presentation] Dynamic changes of O-GlcNAcyaltion in glioma stem cells. (2015)
  • Author(s): Silsirivanit A, Nambu A, Yamasaki Y, Nagai M, Nagayama M, Kobayashi D, Araki N
  • Organizer: 7th Asian Community of Glycoscience and Glycotechnology Conference
  • Place of Presentation: Hotel Taikanso, Miyagi (Japan)
  • Year and Date: 2015-11-12
  • Int'l Joint Research / Invited
• [Presentation] NF1 腫瘍の新規治療ターゲットTCTP-mTOR シグナルの同定と機能解析 (2015)
  • Author(s): 荒木令江 、小林大樹 、長山慈 、中村英夫 、倉津純一
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋市国際会議場（名古屋）
  • Year and Date: 2015-10-08
• [Presentation] 融合プロテオミクスによるグリオーマ幹細胞の維持と分化を制御するペプチドグリカン-インテグリンシグナルの同定と機能解析 (2015)
  • Author(s): 南部晶子、永井美奈子、荒木令江
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋市国際会議場（名古屋）
  • Year and Date: 2015-10-08
• [Presentation] The anti-metastatic activity of chloroquine on cholangiocarcinoma cell lines (2015)
  • Author(s): Diwakar Guragain, Wunchana Seubwai, Sumalee Obchoei, Sopit Wongkham, Norie Araki, Ubon Chaon
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋市国際会議場（名古屋）
  • Year and Date: 2015-10-08
• [Presentation] Comparative proteomics of cholangiocarcinoma cell lines, KKU-M214 and KKU-M214L5 with different metastatic potentials (2015)
  • Author(s): Waraporn Saentaweesuk, Atit Silsirivanit, Kulthida Vaeteewoottacharn, Sopit Wongkham, Chaisiri Wongkham, Norie Araki
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋市国際会議場（名古屋）
  • Year and Date: 2015-10-08
• [Presentation] O-GlcNAcylation regulates the stemness-maintenance of glioma stem cells (2015)
  • Author(s): Atit Silsirivanit, Akiko Niibori-Nambu, Minako Nagai, Megumi Nagayama, Norie Araki
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋市国際会議場（名古屋）
  • Year and Date: 2015-10-08
• [Presentation] Functional Characterization of a Novel NF1-Related Protein TCTP by the Interactome Analysis (2015)
  • Author(s): Daiki Kobayashi , Takaho Tokuda , Megumi Nagayama , Mio Hirayama , Sumio Ohtsuki , Norie Araki
  • Organizer: HUPO2015
  • Place of Presentation: Vancouver Convention Centre (Canada)
  • Year and Date: 2015-09-27
  • Int'l Joint Research
• [Presentation] jPOST: Development of Japan ProteOme STandard Repository/Database (2015)
  • Author(s): Shin Kawano , Yuki Moriya , Shujiro Okuda , Tadashi Yamamoto , Masaki Matsumoto , Daiki Kobayashi, Norie Araki, Akiyasu C. Yoshizawa, Tsuyoshi Tabata, Naoyuki Sugiyama, Susumu Goto, Yasushi Ishihama
  • Organizer: HUPO2015
  • Place of Presentation: Vancouver Convention Centre (Canada)
  • Year and Date: 2015-09-27
  • Int'l Joint Research
• [Presentation] Integrated Proteomics for Identification of the Specific Network in Cancer Stem Cells (2015)
  • Author(s): Norie Araki , Akiko Niibori-Nambu , Atit Silsirivanit , Daiki Kobayashi
  • Organizer: HUPO2015
  • Place of Presentation: Vancouver Convention Centre (Canada)
  • Year and Date: 2015-09-27
  • Int'l Joint Research
• [Presentation] Proteomics Data Analysis Workflow for Accumulated LCMS Files in Public Database (2015)
  • Author(s): Yasushi Ishihama , Tsuyoshi Tabata , Naoyuki Sugiyama , Susumu Goto , Norie Araki , Masaki Matsumoto4, Shujiro Okuda5, Shin Kawano6
  • Organizer: HUPO2015
  • Place of Presentation: Vancouver Convention Centre (Canada)
  • Year and Date: 2015-09-27
  • Int'l Joint Research / Invited
• [Presentation] 融合プロテオミクスによるがん幹細胞の異常シグナルネットワークの解析 (2015)
  • Author(s): 荒木 令江
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本森都市プラザ(熊本)
  • Year and Date: 2015-07-22
  • Invited
• [Presentation] プロテオミクスデータの細胞生物学的な検証法 (2015)
  • Author(s): 小林大樹、荒木令江
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本森都市プラザ(熊本)
  • Year and Date: 2015-07-22
  • Invited
• [Presentation] 全自動2 次元電気泳動装置システムの開発と医療への応用 (2015)
  • Author(s): 矢部 公彦 、西村 宗徳 、後藤 真一 、松永 貴輝 、木下 英樹 、笹尾 明 、荒木 令江
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本森都市プラザ(熊本)
  • Year and Date: 2015-07-22
  • Invited
• [Presentation] セントロメアncRNA 複合体によるクロマチン動態の制御 (2015)
  • Author(s): 谷 時雄 、牟田園 正敏 、井手上 賢 、長 裕紀子 、西岡 詩織 、坂本 実鈴 、石井 浩二郎 、荒木 令江
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本森都市プラザ(熊本)
  • Year and Date: 2015-07-22
  • Invited
• [Presentation] 融合プロテオミクスを用いたグリオーマ幹細胞の分化・維持に関わる糖タンパク質の同定 (2015)
  • Author(s): 南部 晶子、永井 美奈子、荒木 令江
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本森都市プラザ(熊本)
  • Year and Date: 2015-07-22
• [Presentation] 機能プロテオーム解析によって新規に同定した神経線維症1型腫瘍内におけるTCTP と翻訳伸長因子群の相互作用形式 (2015)
  • Author(s): 小林 大樹 、徳田 高穂 、長山 慈 、平山 未央 、大槻 純男 、荒木 令江
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本森都市プラザ(熊本)
  • Year and Date: 2015-07-22
• [Presentation] 日本発の高品質プロテオームデータベース：jPOST (2015)
  • Author(s): 守屋 勇樹 、河野 信 、奥田 修二郎 、山本 格 、松本 雅記 、小林 大樹 、荒木 令江 、吉沢 明康 、五斗 進 、田畑 剛 、杉山 直幸 、石濱 泰
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本森都市プラザ(熊本)
  • Year and Date: 2015-07-22
• [Presentation] 融合プロテオミクスのデータ統合ツールiPEACH によるグリオーマ幹細胞の維持と分化に関わるネットワーク解析 (2015)
  • Author(s): 山崎 義宗 、南部 晶子 、吉沢 明康 、水口 惣平 、小林 大樹 、長山 慈 、永井 美奈子 、Atit Silsirivanit 、河野 信 、佛淵 尚人 、荒木 令江
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本森都市プラザ(熊本)
  • Year and Date: 2015-07-22
• [Presentation] Cholangiocarcinoma cell line; KKU-M214L5 and its parental; KKU-M214 revealed by a label-free proteomic analysis (2015)
  • Author(s): Waraporn Saentaweesuk , Atit Silsirivanit , Kulthida Vaeteewoottacha , Sopit Wongkham , Norie Araki , Chaisiri Wongkha
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本森都市プラザ(熊本)
  • Year and Date: 2015-07-22
• [Presentation] Functional analysis of chloroquine toxicity (2015)
  • Author(s): Diwakar Guragain , Atit Silsirivanit , Wunchana Seubwai , Sopit Wongkham , Ubon Cha’on , Akira Sasao , Ako Sasao , Norie Araki
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本森都市プラザ(熊本)
  • Year and Date: 2015-07-22
• [Presentation] Cancer stem cells up-regulate O-GlcNAcylation systems to maintain their stemness (2015)
  • Author(s): Atit Silsirivanit, Akiko Nambu , Yoshimune Yamasaki , Minako Nagai , Megumi Nagayama ,Daiki Kobayashi , Norie Araki
  • Organizer: 日本プロテオーム学会2015年会
  • Place of Presentation: 熊本森都市プラザ(熊本)
  • Year and Date: 2015-07-22
• [Presentation] 融合プロテオミクスによるがん幹細胞とニッチ標的分子群の解析 (2015)
  • Author(s): 荒木 令江
  • Organizer: 第11回日本臨床プロテオーム研究会
  • Place of Presentation: 国立がん研究センター(東京)
  • Year and Date: 2015-05-23
  • Invited
• [Presentation] 融合プロテオミクスによって同定された神経線維腫症１型（NF1）新規病態関連因子TCTPのNF1腫瘍内における機能と役割 (2015)
  • Author(s): 小林大樹、荒木令江
  • Organizer: 第11回日本臨床プロテオーム研究会
  • Place of Presentation: 国立がん研究センター(東京)
  • Year and Date: 2015-05-23
  • Invited
• [Presentation] Integrated proteomics for identification of specific targets in cancer stem cells (2015)
  • Author(s): Norie Araki
  • Organizer: KHUPO (THE KOREAN HUMAN PROTEOME ORGANIZATION) 15TH ANNUAL INTERNATIONAL PROTEOMICS CONFERENCE
  • Place of Presentation: Seoul National University, Seoul, Korea
  • Year and Date: 2015-03-26 – 2015-03-27
  • Invited
• [Presentation] Development of a fully automated two-dimensional electrophoresis device, Auto-2D, and its application for the integrated proteomics (2014)
  • Author(s): Norie Araki
  • Organizer: 日本電気泳動学会シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2014-10-21 – 2014-10-22
  • Invited
• [Presentation] 融合プロテオミクス：新規治療標的分子ネットワーク同定のためのマルチオミクス解析基盤 (2014)
  • Author(s): 小林 大樹, 荒木 令江
  • Organizer: 第87回日本生化学会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2014-10-15 – 2014-10-18
  • Invited
• [Presentation] Functional proteome analysis of translationally controlled tumor protein (TCTP), a novel Neurofibromatosis type 1 (NF1)-related protein (2014)
  • Author(s): Daiki Kobayashi, Megumi Nagayama, Mio Hirayama, Sumio Ohtsuki, Norie Araki
  • Organizer: 13th human proteome organization World Congress
  • Place of Presentation: IFEMA Madrid Spain
  • Year and Date: 2014-10-05 – 2014-10-08
• [Presentation] Integrated Proteomics for Cancer Stem Cells (2014)
  • Author(s): Norie Araki, Akiko Nambu, Uichi Midorikawa, Souhei Mizuguchi, Daiki Kobayashi, Jun-ichi Kuratsu
  • Organizer: 13th human proteome organization World Congress
  • Place of Presentation: IFEMA Madrid Spain
  • Year and Date: 2014-10-05 – 2014-10-08
• [Presentation] グリオーマ幹細胞の維持と分化に関わるニッチ分子群の融合プロテオミクスによる解析 (2014)
  • Author(s): 荒木令江、南部(新堀) 晶子、緑川宇一、水口惣平、小林大樹、ウイルソン森藤政代、秀拓一郎、中村英夫、倉津純一
  • Organizer: 第73回日本癌学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-25 – 2014-09-27
  • Invited
• [Presentation] 融合プロテオミクスによるがん標的となる修飾分子群の抽出と機能解析 (2014)
  • Author(s): 荒木令江
  • Organizer: レドックス・ライフイノベーション第170委員会 JHUPOサテライトシンポジウ
  • Place of Presentation: 宮崎 シーガイヤ コンベンションセンター
  • Year and Date: 2014-08-22 – 2014-08-23
  • Invited
• [Presentation] 融合プロテオミクスによる神経系腫瘍の細胞内特異的タンパク質の解析 (2014)
  • Author(s): 小林大樹・ 荒木令江
  • Organizer: 日本プロテオーム学会2014年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2014-07-17 – 2014-07-18
  • Invited
• [Presentation] 新規神経線維腫症腫瘍病態関連因子TCTPの機能プロテオーム解析 (2014)
  • Author(s): 小林大樹、長山慈、平山未央、大槻純男、荒木令江
  • Organizer: 日本プロテオーム学会2014年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2014-07-17 – 2014-07-18
• [Presentation] 融合プロテオミクスによる新規NF1病態関連分子ネットワークの同定 (2014)
  • Author(s): 小林大樹・ 平山未央・ 水口惣平・ 荒木令江
  • Organizer: 第62回質量分析総合討論会
  • Place of Presentation: 大阪府吹田市
  • Year and Date: 2014-05-14 – 2014-05-16
  • Invited
• [Presentation] プロテオミクスを基盤とした病態システムズバイオロジー～がん幹細胞の分化メカニズムへの応用～ (2013)
  • Author(s): 荒木令江
  • Organizer: 岡山大学特別講演
  • Place of Presentation: 岡山大学
  • Invited
• [Presentation] 融合プロテオミクスを基盤とした病態システムズバイオロジーによる癌の治療標的検出への挑戦. (2013)
  • Author(s): 荒木令江
  • Organizer: 神戸大学特別講演
  • Place of Presentation: 神戸大学
  • Invited
• [Presentation] Integrated proteomics for identifying the specific signal networks regulated by post-translational modifications in cancer and cancer stem cel.ls. (2013)
  • Author(s): 荒木令江
  • Organizer: ５th ACGG(Asian Commumnications of Glycobiology and Glycotechnology) International Conference.
  • Place of Presentation: タイ コンケン大学
  • Invited
• [Presentation] 融合プロテオミクスによるグリオーマ幹細胞の分化遊動機構と治療ターゲッ ト分 子の解析（Analysis of the differentiation mechanism of glioma initiating cells by the integrated proteomics) (2013)
  • Author(s): 荒木令江、南部(新堀)晶子、緑川宇一、水口惣平、秀拓一郎、小林大樹、ウィルソン森藤政代、菰原義弘、中村英夫、竹屋元裕、倉津純一
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] HIF-1 alphaシグナルを介した細胞競合によるヘテロな口腔癌細胞集団の発育機構（Heterogeneous oral cancer development by competition via HIF-1 alpha signal regulation） (2013)
  • Author(s): 森藤 政代（モリフジ マサヨ）、 南部(新堀) 晶子、 小林 大樹、荒木 令江.
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] An Integrated Proteomics for Extracting Molecular Target of Malignant Gliomas. (2013)
  • Author(s): Norie Araki, Takashi Morikawa, Souhei Mizuguchi, Daiki Kobayashi, Akiko Niibori Nambu, Uichi Midorikawa, Mio Hirayama, Masayo morifuji Wilson, Sin Kawano, Hideo Nakamura, Junichi Kuratsu.
  • Organizer: HUPO 11th Annual World Congress
  • Place of Presentation: パシフィコ横浜
• [Presentation] Integrated Proteomics for the Study of Metastatic Human Tongue Cancer Development in a heterogeneous Microenvironment (2013)
  • Author(s): Masayo Wilson Morifuji, Akiko Niibori Nambu, daiki kobayashi, Norie Araki.
  • Organizer: HUPO 11th Annual World Congress(English Oral)
  • Place of Presentation: パシフィコ横浜
• [Presentation] Integrated Proteomics Identified Novel Activation of Dynein IC2-GR-COX-1 Signaling by Suppression of NF1 Tumor Suppressor Gene Product, Neurofibromin, in Neuronal Cells.. (2013)
  • Author(s): Daiki Kobayashi, Mio Hirayama, Souhei Mizuguchi Takashi Morikawa, Megumi Nagayama, Uichi Midorikawa, Masayo Wilbu-Niibori, Akiyasu Yoshizawa C., Shin Kawano, Norie Araki.
  • Organizer: HUPO 11th Annual World Congress
  • Place of Presentation: パシフィコ横浜
• [Presentation] Integrated Proteomics Identified the Akiko Differentination Niche Induced by glioma Stem Cells. (2013)
  • Author(s): Niiboari Nambu, Uichi Midorikawa, Souhei Mizuguchi, Takuichiro Hide, Minako Nagai, Yoshihiro Komohara, Megumi Nagayama, Mio Hirayama, Daiki Kobayashi, Hideo Nakamura, Motohiro Takeya, Jyunichi Kuratsu, Norie Araki.
  • Organizer: HUPO 11th Annual World Congress
  • Place of Presentation: パシフィコ横浜
• [Presentation] A quick profiling System for Cancer marker Proteins with Diverse Post-Translational Modifications by a Fully Automated 2DE Device, Auto-2D (2013)
  • Author(s): Munenori Nishimura, Uichi Midorikawa, Megumi Nagayama, Daiki Kobayashi, Mio Hirayama, Yoji Murakami, Yoshihiro Wada, Takahisa Imamura, Yutaka Unuma, Norie Araki.
  • Organizer: HUPO 11th Annual World Congress
  • Place of Presentation: パシフィコ横浜
• [Presentation] 融合プロテオミクスによるグリオーマ幹細胞の分化ニッチと悪性化メカニズムの解析 (2013)
  • Author(s): 荒木令江、南部(新堀)晶子、緑川宇一、小林大樹、水口惣平、永井美奈子、秀拓一郎、中村英夫、倉津純一
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 融合プロテオミクスによる神経線維腫症I型（NF1）病態モデル細胞内活性化シグナルDynein IC2-GR-COX-1の同定． (2013)
  • Author(s): 小林大樹、平山未央、水口惣平、森川崇、長山慈、緑川宇一、ウィルソン-森藤政代、南部-新堀晶子、吉沢晶康、河野信、荒木令江.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 全自動2次元電気泳動装置を用いた前立腺がんマーカータンパク質の解析. (2013)
  • Author(s): 西村宗徳、小林大樹、緑川宇一、長山慈、村上洋嗣、河野吉昭、今村隆寿、和田孝浩、鵜沼豊、荒木令江
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 神経系腫瘍の発症メカニズムと治療ターゲットの融合プロテオミクスによる解析 (2013)
  • Author(s): 荒木 令江
  • Organizer: 宮崎大学セミナー
  • Place of Presentation: 宮崎大学
  • Invited
• [Presentation] 融合プロテオミクスによるがん細胞およびがん幹細胞の悪性化ネットワーク抽出と治療ターゲットの検索 (2013)
  • Author(s): 荒木 令江
  • Organizer: 第40回BMS (Biological Mass Spectrometry)コンファレンス
  • Place of Presentation: 宮崎シーガイヤ コンベンションセンター＆シェラトングランデオーシャンリゾート
  • Invited
• [Presentation] 融合プロテオミクスによる異常病態シグナル検出システムの開発と応用 (2013)
  • Author(s): 荒木 令江
  • Organizer: BIO Tech2013 第12回国際バイオテクノロジー展/技術会議
  • Place of Presentation: 東京ビッグサイト
• [Book] 臨床プロテオミクス 医学のあゆみ別冊 (2016)
  • Author(s): 荒木令江, 南部（新堀）晶子, 小林大樹
  • Publisher: 医歯薬出版
• [Book] The frontier of proteomics-based life science and its clinical application. (2015)
  • Author(s): 荒木令江 他
  • Total Pages: 260
  • Publisher: Japan Proteome Society
• [Book] プロテオミクス辞典 (2013)
  • Author(s): 荒木令江
  • Total Pages: 127
  • Publisher: 講談社
• [Book] 神経線維腫症2型，皮膚科臨床アセット15 (2013)
  • Author(s): 荒木令江
  • Total Pages: 384
  • Publisher: 中山書店
• [Remarks] 熊本大学大学院生命科学研究部腫瘍医学分野
  • URL: http://srv02.medic.kumamoto-u.ac.jp/dept/tumor/index.html
• [Remarks] 日本プロテオームデータベースセンター
  • URL: https://www.jhupo.org/database/index.html
• [Remarks] 熊本大学大学院生命科学研究部腫瘍医学分野
  • URL: http://srv02.medic.kumamoto-u.ac.jp/dept/tumor/index.html