Creation of Extracellular Matrices for Duplicating Natural Tissue Structure

• Project/Area Number: 25293404
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Kato Koichi 広島大学, 医歯薬保健学研究院, 教授 (50283875)
• Co-Investigator(Kenkyū-buntansha): NAKAJI Tadashi 富山大学, 大学院理工学研究部, 准教授 (10543217) ; TANIMOTO Kotaro 広島大学, 大学院医歯薬保健学研究院, 教授 (20322240) ; HIRATA Isao 広島大学, 大学院医歯薬保健学研究院, 助教 (40346507)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: 繊維軟骨 / 硝子軟骨 / 間葉系幹細胞 / 組織工学 / 再生医療 / 足場材料 / キメラタンパク質 / 軟骨 / スキャホールド / キメラ蛋白質

Outline of Final Research Achievements

The temporomandibular joint cartilage is made of two distinct tissues including hyaline cartilage in the basal layer and fibrous cartilage in the surface layer. There are various tissues in which such heterotypic tissue structure plays an essential role in their functions. Recently mesenchymal stem cells have been received much attention due to their potentials to regenerate cartilage tissues in patients with a temporomandibular disorder. However, therapeutic outcome does not meet clinical needs because of a difficulty in creating layered structure with hyaline and fibrous cartilages. Our study demonstrated that hyaline and fibrous cartilage-like tissues can be separately induced from mesenchymal stem cells under appropriate culture conditions and that these tissues can be combined by culturing them in an extracellular cage to produce a hyaline-fibrous composite tissue.

Research Products

• [Presentation] Genetically Engineered Proteins as Functional Building Blocks of Tissue Engineering Scaffolds (2015)
  • Author(s): Koichi Kato
  • Organizer: Seminar at Department of Functional Materials in Medicine and Dentistry, Wuerzburg University
  • Place of Presentation: ヴュルツブルグ、ドイツ
  • Year and Date: 2015-05-13
  • Invited
• [Presentation] 間葉系幹細胞の硝子/線維軟骨への分化に及ぼす細胞成長因子及びコラーゲンの影響 (2015)
  • Author(s): 杉野浩孝、金輪真佐美、谷本幸太郎、加藤功一
  • Organizer: 第65回日本歯科理工学会学術講演会
  • Place of Presentation: 仙台
  • Year and Date: 2015-04-11 – 2015-04-12
• [Presentation] 生体分解性ポリエステル結合性増殖因子の分子設計 (2014)
  • Author(s): 西尾文子、平田伊佐雄、加藤功一
  • Organizer: 第36回日本バイオマテリアル学会大会
  • Place of Presentation: 東京
  • Year and Date: 2014-11-17 – 2014-11-18
• [Presentation] Perspectives of Future Dental Materials (2014)
  • Author(s): Koichi Kato
  • Organizer: Seminar & Workshop of CPD in Dental Materials
  • Place of Presentation: スラバヤ、インドネシア
  • Year and Date: 2014-04-25 – 2014-04-27
  • Invited