Studies of the clinical significance and the role of long non coding RNA in oral cancer.

• Project/Area Number: 25293407
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Chiba University
• Principal Investigator: SHIIBA MASASHI 千葉大学, 医学(系)研究科(研究院), 准教授 (20301096)
• Co-Investigator(Kenkyū-buntansha): TANZAWA HIDEKI 千葉大学, 大学院医学研究院, 教授 (50236775) ; KASAMATSU ATSUSHI 千葉大学, 医学部付属病院, 講師 (60375730) ; 小池 博文 千葉大学, 医学部附属病院, 助教 (10595995)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: long non-cording RNA / 口腔扁平上皮癌 / long non-coding RNA

Outline of Final Research Achievements

Cancer-related microRNA(miRNA) has been known in many carcinomas and the possibility of cancer treatment using miRNA has been reported. However, the role of long non coding RNA(IncRNA) in cancer progression is still unknown. The current study, we identified two lncRNA (UCA1 and LINC00256A) by microarray analysis, in order to investigate the role of lncRNA. We made transformed cells by custom shRNA of UCA1 and/or LINC00256A and performed functional analysis. The cells transformed by shUCA1 and/or shLINC00256A showed significantly (P<0.05) decreased invasiveness and migration activities compared with the shMock cells. In addition, we performed microarray analysis using transformed cells and identified target genes. These results indicated that lncRNA may affect tumoral metastasis in OSCCs.