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Exploration of Cold-Adapted Microorganisms to Develop Low-Temperature Bioprocessing Systems

Research Project

Project/Area Number 25303028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section海外学術
Research Field Biofunction/Bioprocess
Research InstitutionKyoto University

Principal Investigator

KURIHARA Tatsuo  京都大学, 化学研究所, 教授 (70243087)

Co-Investigator(Kenkyū-buntansha) 川本 純  京都大学, 化学研究所, 助教 (90511238)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords低温適応微生物 / バイオプロセス / タンパク質生産 / 金属代謝 / 国際研究者交流 / 国際情報交換 / 中華人民共和国 / リン脂質
Outline of Final Research Achievements

From various cold environments, we obtained Pseudomonas mandelii that grows well at low temperatures, bacteria closely related to Paenibacillus odorifer and Bacillus circulans that efficiently reduce iron, and Pseudoalteromonas bacterium that secretes a particular protein in large quantities. For Shewanella sp. HM13, which abundantly produces extracellular membrane vesicles, we established genetic engineering methods. As to Shewanella livingstonensis Ac10 isolated from Antarctic seawater, we found cold-inducible promoters that may be useful for expression of foreign proteins in this strain. We confirmed that PhoE, an outer membrane protein, is involved in iron metabolism of this bacterium and identified functionally important amino acid residues of this protein.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (29 results)

All 2017 2016 2015 2014 2013 Other

All Int'l Joint Research (5 results) Journal Article (6 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 4 results,  Open Access: 1 results) Presentation (18 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Int'l Joint Research] 湖北工業大学/西南大学(中国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Naples “Federico II”(イタリア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 湖北工業大学/西南大学(中国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of Naples “Federico II”(イタリア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] 湖北工業大学(中国)

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Characterization of extracellular membrane vesicles of an Antarctic bacterium, Shewanella livingstonensis Ac10, and their enhanced production by alteration of phospholipid2017

    • Author(s)
      Yokoyama F, Kawamoto J, Imai T, Kurihara T
    • Journal Title

      Extremophiles

      Volume: 印刷中 Issue: 4 Pages: 723-731

    • DOI

      10.1007/s00792-017-0937-z

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of a versatile method for targeted gene deletion and insertion by using the pyrF gene in the psychrotrophic bacterium, Shewanella livingstonensis Ac102016

    • Author(s)
      Ito T, Gong C, Kawamoto J, Kurihara T
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 122 Issue: 6 Pages: 645-651

    • DOI

      10.1016/j.jbiosc.2016.06.004

    • NAID

      40021044862

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A phosphoporin homolog plays a critical role in the dissimilatory iron-respiration linked to iron(III) reduction by a cold-adapted bacterium, Shewanella livingstonensis2016

    • Author(s)
      Ohke Y, Maruyama S, Tarui J, Wang Y, Kawamoto J, Kurihara T
    • Journal Title

      Trace Nutr. Res.

      Volume: 33 Pages: 63-72

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A phosphoporin homolog plays a critical role in the dissimilatory iron-respiration linked to iron(III) reduction by a cold-adapted bacterium, Shewanella livingstonensis Ac102016

    • Author(s)
      Ohke Y, Maruyama S, Tarui J, Wang Y, Kawamoto J, Kurihara T
    • Journal Title

      Trace Nutr. Res.

      Volume: 33 Pages: 63-72

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Alkyl hydroperoxide reductase enhances the growth of Leuconostoc mesenteroides lactic acid bacteria at low temperatures.2015

    • Author(s)
      Goto S, Kawamoto J, Sato SB, Iki T, Watanabe I, Kudo K, Esaki N, Kurihara T.
    • Journal Title

      AMB Express

      Volume: in press Issue: 1

    • DOI

      10.1186/s13568-015-0098-3

    • NAID

      120005587816

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 低温菌のタンパク質と脂質2014

    • Author(s)
      川本純、栗原達夫
    • Journal Title

      CSJ Current Review 17 極限環境の生体分子

      Volume: 17 Pages: 55-61

    • Related Report
      2014 Annual Research Report
  • [Presentation] Analysis of a protein secretion mechanism via the membrane vesicle production of a cold-adapted bacterium, Shewanella sp. HM132017

    • Author(s)
      Chen C, Kawai S, Kawamoto J, Imai T, Kurihara T
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 の鉄呼吸における Phosphoporin ホモログの生理機能2016

    • Author(s)
      大毛淑恵、丸山沙織、樽井惇、王玉、川本純、栗原達夫
    • Organizer
      第17回 極限環境生物学会年会
    • Place of Presentation
      東京工業大学(横浜市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 による菌体外膜小胞の生産とエイコサペンタエン酸生産能の欠損が膜小胞分泌におよぼす影響2016

    • Author(s)
      横山文秋、川本純、今井友也、小川拓哉、栗原達夫
    • Organizer
      第89回 日本生化学会大会
    • Place of Presentation
      仙台国際センター(仙台市)
    • Year and Date
      2016-09-27
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Presentation] Analysis of protein secretion system of a membrane-vesicle producing cold-adapted bacterium, Shewanella sp. HM132016

    • Author(s)
      Chen C, Kawai S, Kawamoto J, Imai T, Kurihara T
    • Organizer
      Extremophiles 2016
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular characterization of eicosapentaenoic acid-containing membrane vesicles produced by a psychrotrophic bacterium, Shewanella livingstonensis Ac102016

    • Author(s)
      Yokoyama F, Kawamoto J, Imai T, Ogawa T, Kurihara T
    • Organizer
      Extremophiles 2016
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Physiological function of polyunsaturated fatty acids in microbial cold and high-pressure adaptation2016

    • Author(s)
      Kawamoto J, Kurihara T
    • Organizer
      5th International Workshop on Deep-Sea Microbiology
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Shewanella livingstonensis Ac10 における三価鉄還元機構の解析2016

    • Author(s)
      大毛淑恵、丸山沙織、樽井惇、王玉、川本純、栗原達夫
    • Organizer
      第33回 日本微量栄養素学会学術集会
    • Place of Presentation
      京都リサーチパーク(京都市)
    • Year and Date
      2016-06-25
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 の菌体外膜小胞の特性とエイコサペンタエン酸が小胞形成に与える影響の解析2016

    • Author(s)
      横山文秋、川本純、今井友也、小川拓也、栗原達夫
    • Organizer
      第63回 日本生化学会 近畿支部例会
    • Place of Presentation
      神戸薬科大学(神戸市)
    • Year and Date
      2016-05-21
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 のエイコサペンタエン酸生合成酵素の細胞分裂部位への局在性と複合体形成の解析2015

    • Author(s)
      小倉隆太郎、趙賢南、川本純、栗原達夫
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県 岡山市)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Membrane microdomain enriched in eicosapentaenoic acid-containing phospholipids in Shewanella livingstonensis Ac102014

    • Author(s)
      栗原達夫
    • Organizer
      Japan-Italy Joint Symposium
    • Place of Presentation
      東大寺総合文化センター(奈良県 奈良市)
    • Year and Date
      2014-11-05
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 の金属呼吸に関与する外膜輸送タンパク質の解析2014

    • Author(s)
      丸山沙織、樽井淳、王玉、川本純、栗原達夫
    • Organizer
      第15回極限環境生物学会年会
    • Place of Presentation
      今帰仁村コミュニティーセンター(沖縄県 国頭郡)
    • Year and Date
      2014-11-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 におけるEPA生合成酵素の局在性と相互作用解析2014

    • Author(s)
      小倉隆太郎、趙賢南、川本純、栗原達夫
    • Organizer
      第15回極限環境生物学会年会
    • Place of Presentation
      今帰仁村コミュニティーセンター(沖縄県 国頭郡)
    • Year and Date
      2014-11-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 の細胞分裂サイトへのエイコサペンタエン酸生合成酵素の局在2014

    • Author(s)
      小倉隆太郎、趙賢南、川本純、栗原達夫
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府 京都市)
    • Year and Date
      2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 の飽和脂肪酸含有リン脂質生合成に関与する新規アシルトランスフェラーゼの解析2014

    • Author(s)
      豊竹洋佑、趙賢南、川本純、江崎信芳、栗原達夫
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府 京都市)
    • Year and Date
      2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Localization of 1-acyl-sn-glycerol-3-phosphate acyltransferase 1 (PlsC1) of a psychrotrophic bacterium, Shewanella livingstonensis Ac10, at the middle of cells and its physiological role in cell division2013

    • Author(s)
      Cho HN, Kasai W, Kawamoto J, Esaki N, Kurihara T
    • Organizer
      EMBO|EMBL Symposium New Approaches and Concepts in Microbiology
    • Place of Presentation
      Heidelberg
    • Related Report
      2013 Annual Research Report
  • [Presentation] Physiological role of 1-acyl-sn-glycerol-3-phosphate acyltransferase 1 (PlsC1) in cell division of a psychrotrophic bacterium in Shewanella livingstonensis Ac10 at low temperatures

    • Author(s)
      Cho HN, Kasai W, Kawamoto J, Esaki N, Kurihara T
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Subcellular localization of 1-acyl-sn-glycerol-3-phosphate acyltransferase responsible for the synthesis of EPA-containing phospholipids in Shewanella livingstonensis Ac10

    • Author(s)
      Cho HN, Kasai W, Kawamoto J, Esaki N, Kurihara T
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 のエイコサペンタエン酸生合成酵素の局在性解析

    • Author(s)
      小倉隆太郎、趙賢南、川本純、栗原達夫
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2022-02-16  

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