Surveys on factors of onset and progression for CKDue in Sri Lanka

• Project/Area Number: 25305021
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Hygiene and public health
• Research Institution: Kyoto University
• Principal Investigator: HARADA KOUJI 京都大学, 医学(系)研究科(研究院), 准教授 (80452340)
• Co-Investigator(Kenkyū-buntansha): 小泉 昭夫 京都大学, 医学(系)研究科(研究院), 教授 (50124574)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 環境衛生学 / 分析化学 / 国際保健 / 慢性腎臓病 / 疫学 / 化学分析 / 環境衛生学分 / スリランカ / 農民 / 農薬 / 生活習慣病 / 遺伝疫学

Outline of Final Research Achievements

In Sri Lanka in South Asia, chronic kidney disease has rapidly increased in the eastern part and the northern part of the island of Ceylon since the 1990s. We have investigated possible candidate agents and genetic factors. SLC13A3, KCNJ10 and LAMB2 were found to be possible candidates. Aristolochia has been used as a plant biologics with renal toxicity. In order to investigate the involvement, the urine was assayed for alistolochic acid. As a result, no detectable amount of the alistolochic acid was found. Also neonicotinoid was analyzed to reveal exposure to pesticide, while there was no difference between case and controls. And electron microscopic observations of the histological tissues were performed.The observation of electron microscopic observation of renal tissues showed no evidence supporting the heavy metal toxicity in the pathogenesis of CKDu .

Research Products

• [Int'l Joint Research] ペラデニア大学(スリランカ)
• [Int'l Joint Research] シドニー大学/チャールズ・スタート大学(オーストラリア)
• [Int'l Joint Research] ペラデニヤ大学(スリランカ)
• [Int'l Joint Research] シドニー大学/チャールズ・スタート大学(オーストラリア)
• [Journal Article] Neonicotinoid concentrations in urine from chronic kidney disease patients in the North Central Region of Sri Lanka (2016)
  • Author(s): Risako Kabata, Shanika Nanayakkara, STMLD Senevirathna, Kouji H. Harada, Rohana Chandrajith, Toshiaki Hitomi, Tilak Abeysekera, Takumi Takasuga and Akio Koizumi
  • Journal Title: Journal of Occupational Health Volume: 58 Issue: 1 Pages: 128-133
  • DOI: 10.1539/joh.15-0140-BR
  • NAID: 130005127672
  • ISSN: 1341-9145, 1348-9585
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Biological Monitoring of Human Exposure to Neonicotinoids Using Urine Samples, and Neonicotinoid Excretion Kinetics. (2016)
  • Author(s): Harada KH, Tanaka K, Sakamoto H, Imanaka M, Niisoe T, Hitomi T, Kobayashi H, Okuda H, Inoue S, Kusakawa K, Oshima M, Watanabe K, Yasojima M, Takasuga T, Koizumi A.
  • Journal Title: PLoS One. Volume: 11(1) Issue: 1 Pages: e0146335-e0146335
  • DOI: 10.1371/journal.pone.0146335
  • NAID: 120005763403
  • Peer Reviewed / Open Access
• [Journal Article] Whole-exome sequencing reveals genetic variants associated with chronic kidney disease characterized by tubulointerstitial damages in North Central Region, Sri Lanka (2015)
  • Author(s): Nanayakkara S, Senevirathna S, Parahitiyawa NB, Abeysekera T, Chandrajith R, Ratnatunga N, Hitomi T, Kobayashi H, Harada KH, Koizumi A
  • Journal Title: Environ Health Prev Med. Volume: 20(5) Issue: 5 Pages: 354-9
  • DOI: 10.1007/s12199-015-0475-1
  • NAID: 50009736401
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] An Integrative Study of the Genetic, Social and Environmental Determinants of Chronic Kidney Disease Characterized by Tubulointerstitial Damages in the North Central Region of Sri Lanka. (2014)
  • Author(s): Nanayakkara S, Senevirathna S, Abeysekera T, Chandrajith R, Ratnatunga N, Gunarathne E, Yan J, Hitomi T, Muso E, Komiya T, Harada KH, Liu W, Kobayashi H, Okuda H, Sawatari H, Matsuda F, Yamada R, Watanabe T, Miyataka H, Himeno S, Koizumi A.
  • Journal Title: J Occup Health Volume: 56 Pages: 28-38
  • NAID: 130004848049
  • Peer Reviewed
• [Presentation] スリランカ中北部州のCKD患者における尿中ネオニコチノイド濃度の評価 (2016)
  • Author(s): 加畑理咲子、原田浩二、小泉昭夫
  • Organizer: 第86回 日本衛生学会総会
  • Place of Presentation: 旭川
  • Year and Date: 2016-05-11
• [Presentation] スリランカ中北部乾燥地域における慢性腎臓病の原因究明 (2016)
  • Author(s): 原田浩二、Nanayakkara Shanika、小泉昭夫
  • Organizer: 第15回 分子予防環境医学研究会
  • Place of Presentation: 自治医科大学
  • Year and Date: 2016-01-29
  • Invited
• [Presentation] ネオニコチノイド農薬のヒト体内動態と曝露評価 (2015)
  • Author(s): 原田浩二、田中惠子、坂本裕子、今中美栄、新添多聞、人見敏明、小林果、奥田裕子、井上純子、草川浩一、大島匡世、渡邉清彦、八十島誠、高菅卓三、小泉昭夫
  • Organizer: 第24回 日本環境化学会討論会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2015-06-24
• [Presentation] LC-MS/MSを用いたヒト尿中ネオニコチノイド系農薬およびその代謝物の高感度分析法の開発 (2015)
  • Author(s): 渡邉清彦、八十島誠、高菅卓三、原田浩二、小泉昭夫
  • Organizer: 第24回 日本環境化学会討論会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2015-06-24
• [Presentation] ヒトにおけるネオニコチノイド農薬の体内動態の検討 (2015)
  • Author(s): 原田浩二、新添多聞、田中惠子、坂本裕子、今中美栄、大島匡世、草川浩一、奥田裕子、小林果、小泉昭夫
  • Organizer: 第85回 日本衛生学会総会
  • Place of Presentation: 和歌山
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] スリランカ農民の慢性腎臓病関連院試の探索 (2014)
  • Author(s): 人見敏明、Shanika Nanayakkara、STMLD Senevirathna、原田浩二、小林果、宮高透喜、姫野誠一郎、山内博、小泉昭夫
  • Organizer: 第54回 近畿産業衛生学会
  • Place of Presentation: 近畿大学
  • Year and Date: 2014-11-15
• [Presentation] スリランカ農民の慢性腎臓病 (2013)
  • Author(s): 人見敏明、Shanika Nanayakkara、STMLD Senevirathna、原田浩二、小林果、宮高透喜、姫野誠一郎、小泉昭夫
  • Organizer: 第53回 近畿産業衛生学会
  • Place of Presentation: 京都大学
• [Remarks] スリランカで多発する慢性腎疾患の原因究明
  • URL: http://hes.med.kyoto-u.ac.jp/SriLanka/
• [Remarks] スリランカで多発する慢性腎疾患の原因究明
  • URL: http://hes.med.kyoto-u.ac.jp/SriLanka/index.html