Effect of BCL11B and its mutations on the radiation response: analysis of a p53-MDM2 feed-back loop
Project/Area Number |
25340027
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Risk sciences of radiation and chemicals
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Research Institution | Niigata University |
Principal Investigator |
OBATA Miki 新潟大学, 医学部, 教務職員 (00420307)
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Co-Investigator(Kenkyū-buntansha) |
MISHIMA Yukio 新潟大学, 医歯学系, 准教授 (30142029)
KOMINAMI Ryo 新潟大学, 医歯学総合研究科, 研究員 (40133615)
KATSURAGI Yoshinori 新潟大学, 医歯学系, 助教 (60401759)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | BCL11B / がん抑制遺伝子 / SWI/SNF複合体 / 放射線応答 / p53-MDM2フィードバックループ / がん抑制因子 / SWI/SNIF複合体 / グリセロール密度勾配遠心法 / DNAチップ解析 |
Outline of Final Research Achievements |
Comprehensive profiling of cancer genomics has revealed that somatic mutations of genes encoding SWI/SNF chromatin complexes are a common driver mechanism of tumorigenesis. Since BCL11B was reported to affect transcription as a SWI/SNF component, we examined whether BCL11B incorporated into SWI/SNF complexes in HCT116 cells by transfecting BCL11B expression plasmids into cells, followed by glycerol density sedimentation analysis of the cell lysates. Most of the wild-type BCL11B but not the mutant-type BCL11B proteins were detected in the fractions of large complexes with BRG1 and other SWI/SNF components, indicating that BCL11B is the subunit of SWI/SNF complexes. Initially, we attempted to investigate whether the mutation of BCL11B affects the oscillation of p53-MDM2 by gamma ray irradiation. We failed to detect the difference of oscillation of p53-MDM2 between in the presence of wild-type and mutant-type BCL11B.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Human T-cell leukemia virus type 1 Tax oncoprotein represses the expression of the BCL11B tumor suppressor in T-cells2015
Author(s)
Takachi T, Takahashi M, Takahashi-Yoshita M, Higuchi M, Obata M, Mishima Y, Okuda S, Tanaka Y, Matsuoka M, Saitoh A, Green PL, Fujii M.
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Journal Title
Cance Science
Volume: 106(4)
Issue: 4
Pages: 461-465
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Bcl11b SWI/SNF-complex subunit modulates intestinal adenoma and regeneration after γ-irradiation through Wnt/β-catenin pathway.2015
Author(s)
Sakamaki A, Katsuragi Y, Otsuka K, Tomita M, Obata M, Iwasaki T, Abe M, Sato T, Ochiai M, Sakuraba Y, Aoyagi Y, Gondo Y, Sakimura K, Nakagama H, Mishima Y, Kominami R
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Journal Title
Carcinogenesis
Volume: 36
Issue: 6
Pages: 622-631
DOI
Related Report
Peer Reviewed
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[Journal Article] Successful management of severe intrahepatic cholestasis of pregnancy: report of a first Japanese case.2014
Author(s)
Kamimura K, Abe H, Kamimura N, Yamaguchi M, Mamizu M, Ogi K, Takahashi Y, Mizuno K, Kamimura H, Kobayashi Y, Takeuchi M, Yoshida K, Yamada K, Enomoto T, Takakuwa K, Nomoto M, Obata M, Katsuragi Y, Mishima Y, Kominami R, Kamimura T, Aoyagi Y.
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Journal Title
BMC Gastroenterol.
Volume: 14
Issue: 1
Pages: 160-4
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Bcl11b transcription factor plays a role in the maintenance of the ameloblast-progenitors in mouse adult maxillary incisors.2013
Author(s)
Katsuragi Y, Anraku J, Nakatomi M, Ida-Yonemochi H, Obata M, Mishima Y, Sakuraba Y, Gondo Y, Kodama Y, Nishikawa A, Takagi R, Ohshima H, Kominami R.
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Journal Title
Mechanism and Development
Volume: 130
Issue: 9-10
Pages: 482-492
DOI
Related Report
Peer Reviewed
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