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High-pressure microscopy for controlling the structure of protein filaments

Research Project

Project/Area Number 25390037
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nanobioscience
Research InstitutionKyoto University

Principal Investigator

Nishiyama Masayoshi  京都大学, 白眉センター, 准教授 (10346075)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords高圧力 / 顕微鏡 / 微小管 / べん毛 / ナノバイオ / 分子モーター / 分子機械 / タンパク質水和 / 高圧力顕微鏡 / 水和 / べん毛繊維 / 超らせん構造
Outline of Final Research Achievements

We have developed a high-pressure microscope that enables to observe the structure and function of protein molecules. In this research project, we have studied the conformational changes of protein filaments at high-pressure. By using this system, we were successful to change the helical structure of flagellar filaments and microtubule dynamics.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (41 results)

All 2016 2015 2014 2013 Other

All Journal Article (15 results) (of which Peer Reviewed: 11 results,  Acknowledgement Compliant: 4 results,  Open Access: 3 results) Presentation (25 results) (of which Int'l Joint Research: 2 results,  Invited: 14 results) Book (1 results)

  • [Journal Article] Reversible morphological control of tubulin-encapsulating giant liposomes by hydrostatic pressure2016

    • Author(s)
      Hayashi M., Nishiyama M., Kazayama Y., Toyota T., Harada Y. & Takiguchi K.
    • Journal Title

      Langmuir

      Volume: 32 Issue: 15 Pages: 3794-3802

    • DOI

      10.1021/acs.langmuir.6b00799

    • NAID

      120006457110

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 高圧力技術を用いた生命活動操作イメージング2016

    • Author(s)
      西山雅祥
    • Journal Title

      現代化学

      Volume: 538 Pages: 48-49

    • Related Report
      2015 Annual Research Report
  • [Journal Article] High-pressure microscopy for studying molecular motors.2015

    • Author(s)
      Nishiyama M.
    • Journal Title

      Subcellular Biochemistry

      Volume: 72 Pages: 593-611

    • DOI

      10.1007/978-94-017-9918-8_27

    • ISBN
      9789401799171, 9789401799188
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sodium-driven energy conversion for flagellar rotation of the earliest divergent hyperthermophilic bacterium2015

    • Author(s)
      Takekawa N., Nishiyama M., Kaneseki T., Kanai T., Atomi H., Kojima S. & Homma M.
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 12711-12711

    • DOI

      10.1038/srep12711

    • NAID

      120005627626

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Direct Observation of the Motility of Biological Nanomachines at High Pressure2015

    • Author(s)
      西山雅祥
    • Journal Title

      The Review of High Pressure Science and Technology

      Volume: 25 Issue: 2 Pages: 126-135

    • DOI

      10.4131/jshpreview.25.126

    • NAID

      130005075755

    • ISSN
      0917-639X, 1348-1940
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] タンパク質分子機械の力学変調計測2015

    • Author(s)
      西山雅祥
    • Journal Title

      機械の研究

      Volume: 67 Pages: 1067-1074

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] 高圧力顕微鏡法による細菌運動観察2015

    • Author(s)
      西山雅祥
    • Journal Title

      高圧バイオサイエンスとバイオテクノロジー

      Volume: 印刷中

    • NAID

      120005670891

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] タンパク質間相互作用を力学刺激で操作する2014

    • Author(s)
      西山雅祥
    • Journal Title

      化学と生物

      Volume: 49 Pages: 782-784

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Viewing the rotation of molecular motors at high pressure.2014

    • Author(s)
      Okuno D., Nishiyama M., Noji H.
    • Journal Title

      Asia Pacific Physics Newsletter

      Volume: 3 Pages: 25-25

    • Related Report
      2014 Research-status Report
  • [Journal Article] High hydrostatic pressure induces CCW to CW reversals of the Escherichia coli flagellar motor2013

    • Author(s)
      M.Nishiyama, Y.Sowa, Y.Kimura, M. Homma, A. Ishijima, M. Terazima
    • Journal Title

      J.Bacterio.

      Volume: 195 Issue: 8 Pages: 1809-1814

    • DOI

      10.1128/jb.02139-12

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Glycine insertion makes yellow fluorescent protein sensitive to hydrostatic pressure.2013

    • Author(s)
      Watanabe TM, Imada K, Yoshizawa K, Nishiyama M, Kato C, Abe F, Morikawa TJ, Kinoshita M, Fujita H, Yanagida T.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 8 Pages: e73212-e73212

    • DOI

      10.1371/journal.pone.0073212

    • NAID

      120005328377

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Single molecule analysis of the rotation of F1-ATPase under high hydrostatic pressure2013

    • Author(s)
      Daichi Okuno, Masayoshi Nishiyama and Hiroyuki Noji
    • Journal Title

      Biophysical Journal

      Volume: 105 Issue: 7 Pages: 1635-1642

    • DOI

      10.1016/j.bpj.2013.08.036

    • NAID

      120005328380

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 高圧力顕微鏡2013

    • Author(s)
      西山雅祥, 木村佳文
    • Journal Title

      LTMセンター誌

      Volume: 22 Pages: 18-27

    • NAID

      120005289856

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 細胞内の水で生命活動を操る! -高圧力下で観るタンパク質水和変調イメージング-2013

    • Author(s)
      西山雅祥, 曽和義幸
    • Journal Title

      化学

      Volume: 68 Pages: 31-36

    • NAID

      120005328379

    • Related Report
      2013 Research-status Report
  • [Journal Article] High Hydrostatic Pressure Induces Reversal Rotations of the Bacterial Flagellar Motor!?2013

    • Author(s)
      西山雅祥
    • Journal Title

      Seibutsu Butsuri

      Volume: 53 Issue: 5 Pages: 264-265

    • DOI

      10.2142/biophys.53.264

    • NAID

      10031191850

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 高圧力顕微鏡法による細菌べん毛の構造変化イメージング2016

    • Author(s)
      西山雅祥, 曽和義幸
    • Organizer
      第63回応用物理学会 春期学術講演会
    • Place of Presentation
      東京工業大学(東京都目黒区大岡山)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] High-pressure microscopy for studying molecular motor and cytoskeleton2016

    • Author(s)
      Nishiyama M.
    • Organizer
      8th International Meeting on Biomolecules under Pressure
    • Place of Presentation
      Dortmund (Germany)
    • Year and Date
      2016-02-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-pressure microscopy for manipulating cellular architecture and functions2015

    • Author(s)
      Nishiyama M.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県・金沢市角間町)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] タンパク質分子機械の力学変調イメージング2015

    • Author(s)
      西山雅祥
    • Organizer
      北海道大学フロンティア化学教育研究センター
    • Place of Presentation
      北海道大学(北海道・札幌市北区)
    • Year and Date
      2015-08-17
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] High-pressure microscopy for studying bacterial motility machinery2015

    • Author(s)
      Nishiyama M.
    • Organizer
      Microbiology and Biogeochemistry of the Deep Sea and the Deep Biosphere
    • Place of Presentation
      Shanghai (China)
    • Year and Date
      2015-06-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高圧力顕微鏡の開発と生体運動変調2015

    • Author(s)
      西山雅祥, 八木俊樹
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      国立京都国際会館(京都府京都市 左京区宝ヶ池)
    • Year and Date
      2015-05-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] タンパク質分子機械の力学変調コントロール2015

    • Author(s)
      西山雅祥
    • Organizer
      理研シンポ・細胞システムの動態と論理Ⅶ
    • Place of Presentation
      理化学研究所(埼玉県・和光市広沢)
    • Year and Date
      2015-04-02
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 深海性Shewanella属細菌由来シトクロムcの圧力適応機構2015

    • Author(s)
      政成美沙, 西山雅祥, 三本木至宏
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] クラミドモナス非運動性変異株の力学コントロール2015

    • Author(s)
      八木俊樹, 西山雅祥
    • Organizer
      第62回応用物理学会年会
    • Place of Presentation
      日本大学
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Bacterial Motility Measured by a Miniature Chamber for High-pressure Microscopy2015

    • Author(s)
      Nishiyama M. & Kojima S.
    • Organizer
      59th Annual Meeting of Biophysical Society
    • Place of Presentation
      Baltimore, USA
    • Year and Date
      2015-02-07 – 2015-02-11
    • Related Report
      2014 Research-status Report
  • [Presentation] チューブリン封入ジャイアントリポソームの温度・静水圧変化による可逆的形態制御2015

    • Author(s)
      林真人, 西山雅祥, 風山祐輝, 豊田太郎, 滝口金吾
    • Organizer
      2015年生体運動合同班会議
    • Place of Presentation
      学習院大学
    • Year and Date
      2015-01-07 – 2015-01-09
    • Related Report
      2014 Research-status Report
  • [Presentation] 力学刺激で細胞運動を操作する2014

    • Author(s)
      西山雅祥
    • Organizer
      第103回生命機能研究科コロキウム
    • Place of Presentation
      大阪大学
    • Year and Date
      2014-12-10
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 高圧力を用いたタンパク質分子機械の運動変調イメージング2014

    • Author(s)
      西山雅祥
    • Organizer
      第55回高圧討論会
    • Place of Presentation
      徳島大学
    • Year and Date
      2014-11-22 – 2014-11-24
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Manipulating the molecular machinery by water molecules of the hydration.2014

    • Author(s)
      Nishiyama M.
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] High pressure induces flagellar bending movements in Chlamydomonas paralyzed mutants2014

    • Author(s)
      Yagi T. & Nishiyama M.
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Reversible morphological control of tubulin-encapsulated giant-liposomes induced by change of hydrostatic pressure and temperature2014

    • Author(s)
      Hayashi M., Nishiyama M., Kazayama Y., Toyota T. &Takiguchi K.
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Development of a New High-pressure Microscope.2014

    • Author(s)
      Nishiyama M.
    • Organizer
      Manipulation of Integrated Molecular Reaction Processes in living cell
    • Place of Presentation
      The Consortium of Universities in Kyoto
    • Year and Date
      2014-09-02
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 細胞内で働く分子マシンを細胞外からあやつる2014

    • Author(s)
      西山雅祥
    • Organizer
      法政大学ナノテクセミナー
    • Place of Presentation
      法政大学
    • Year and Date
      2014-08-07
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Single molecule analysis of ATP-driven molecular motors at high pressure.2014

    • Author(s)
      Nishiyama M.
    • Organizer
      7th International Meeting on Biomolecules under Pressure
    • Place of Presentation
      Montpelier, France
    • Year and Date
      2014-07-15 – 2014-07-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] タンパク質分子機械の力学変調イメージング2014

    • Author(s)
      西山雅祥
    • Organizer
      第259回光ナノサイエンス特別講義
    • Place of Presentation
      奈良科学技術大学院大学
    • Year and Date
      2014-04-30
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 極限環境で駆動する生体ナノマシンの直接観察

    • Author(s)
      西山雅祥
    • Organizer
      法政大学ナノテクセミナー
    • Place of Presentation
      法政大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 高圧力を用いたタンパク質分子機械の構造変化イメージング

    • Author(s)
      西山雅祥
    • Organizer
      日本機械学会2013年度大会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 高圧力下で見られるべん毛繊維の動的多型性

    • Author(s)
      西山雅祥, 曽和義幸
    • Organizer
      日本生物物理学会
    • Place of Presentation
      京都国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] バクテリアべん毛繊維のダイナミック構造変化イメージング

    • Author(s)
      西山雅祥, 曽和義幸
    • Organizer
      2014年 生体運動研究合同班会議
    • Place of Presentation
      千葉大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 高圧力顕微鏡の開発と生体分子機械の運動変調

    • Author(s)
      西山雅祥, 曽和義幸
    • Organizer
      第61回 応用物理学会 春期学術講演会
    • Place of Presentation
      青山学院大学
    • Related Report
      2013 Research-status Report
  • [Book] 高圧バイオサイエンスとバイオテクノロジー2015

    • Author(s)
      西山雅祥
    • Publisher
      三恵社
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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