Study on liquid-liquid transtion of protein hydaration water by collective dynamics and liquid strcuture analysis

• Project/Area Number: 25390130
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Quantum beam science
• Research Institution: Fukuoka University
• Principal Investigator: Yoshida Koji 福岡大学, 理学部, 助教 (00309890)
• Co-Investigator(Renkei-kenkyūsha): YAMAGUCHI TOSHIO 福岡大学, 理学部, 教授 (70158111)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: タンパク質 / 水和 / X線回折 / 中性子散乱 / X線散乱 / Ｘ線回折

Outline of Final Research Achievements

Collective dynamics and structure of hydration water for hydrated proteins and polypeptides were investigated by neutron and X-ray scatterings in the temperature range of 298 - 180 K. Hydration structure of proteins and amino acids confined in mesoporous silica and nano-water domain formed in ionic liquid-water mixture. The relation between protein dynamics and water was clarified and the water role in protein function was discussed.

Research Products

• [Journal Article] Structure and collective dynamics of hydrated anti-freeze protein type III from 180 K to 298 K by X-ray diffraction and inelastic X-ray scatterin (2016)
  • Author(s): K. Yoshida, A. Q. R. Baron, H. Uchiyama, S. Tsutsui, T. Yamaguchi
  • Journal Title: J. Chem. Phys. Volume: 144 Issue: 13 Pages: 134505-134505
  • DOI: 10.1063/1.4944987
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Thermal properties and hydration structure of poly-L-lysine, polyglycine, and lysozyme (2016)
  • Author(s): K. Yoshida, A. Tashiro, T. Yamaguchi
  • Journal Title: J. Mol. Liquids Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Relationship between Structural Relaxation, Shear Viscosity, and Ionic Conduction of LiPF6/Propylene Carbonate Solutions (2015)
  • Author(s): T. Yamaguchi, S. Seki他
  • Journal Title: J. Phys. Chem. B Volume: 119 Issue: 51 Pages: 15675-15682
  • DOI: 10.1021/acs.jpcb.5b08701
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 量子ビームで見るタンパク質の水和現象 (2015)
  • Author(s): 吉田 亨次, 山口 敏男
  • Journal Title: 分析化学 Volume: 64 Issue: 4 Pages: 283-293
  • DOI: 10.2116/bunsekikagaku.64.283
  • NAID: 130005060614
  • Peer Reviewed
• [Journal Article] Dynamic Properties of Water Confined in Sephadex G15 Gel by Quasi-Elastic Neutron Scattering and Neutron Spin Echo Measurements (2014)
  • Author(s): K. Ito, K. Yoshida, M.-C. Bellissent-Funel, T. Yamaguchi
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 87 Issue: 5 Pages: 603-608
  • DOI: 10.1246/bcsj.20130328
  • NAID: 130004153301
  • ISSN: 0009-2673, 1348-0634
  • Peer Reviewed
• [Journal Article] Neutron Brillouin scattering of hydrated β-lactoglobulin (2014)
  • Author(s): K. Yoshida, T. Yamaguchi, D. Kawana, T. Yokoo, S. Itoh
  • Journal Title: MLF Annual Report 2013 Volume: 3 Pages: 33-34
• [Journal Article] A study of alcohol and temperature effects on aggregation of β-lactoglobulin by viscosity and small-angle X-ray scattering measurements (2013)
  • Author(s): K. Yoshida, Y. Fukushima, T. Yamaguchi
  • Journal Title: J. Mol. Liquids Volume: 189 Pages: 1-8
  • DOI: 10.1016/j.molliq.2013.06.022
  • Peer Reviewed
• [Journal Article] Thermal Behavior and Structure of Low-temperature Water Confined in Sephadex G15 Gel by Differential Scanning Calorimetry and X-ray Diffraction Method (2013)
  • Author(s): K. Ito, K. Yoshida, K. Ujimoto, T. Yamaguchi
  • Journal Title: Analytical Sciences Volume: 29 Issue: 3 Pages: 353-359
  • DOI: 10.2116/analsci.29.353
  • NAID: 10031165322
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Thermal behavior, structure, and dynamics of low-temperature water confined in mesoporous organosilica by differential scanning calorimetry, X-ray diffraction, and quasi-elastic neutron scattering (2013)
  • Author(s): M. Aso, K. Ito, H. Sugino, K. Yoshida, T. Yamada, O. Yamamuro, S. Inagaki, T. Yamaguchi
  • Journal Title: Pure Appl. Chem Volume: 85 Issue: 1 Pages: 289-305
  • DOI: 10.1351/pac-con-12-06-02
  • Peer Reviewed
• [Presentation] ポリペプチドの集団ダイナミクスにおける水和効果 (2015)
  • Author(s): 吉田 亨次, 山口 敏男, 細川 伸也, A.Q.R. Baron
  • Organizer: 第38回溶液化学シンポジウム
  • Place of Presentation: 高知
  • Year and Date: 2015-10-21
• [Presentation] Collective dynamics of hydrated protein and polypeptides by Inelastic X-ray Scattering (2015)
  • Author(s): K. Yoshida, T. Yamaguchi, S. Hosokawa, A. Q. R. Baron
  • Organizer: EMLG-JMLG annual meeting 2015
  • Place of Presentation: Rostock
  • Year and Date: 2015-09-06
  • Int'l Joint Research
• [Presentation] Stabilization of α-Helix structure of Ribonuclease A induced by Alkylammonium Nitrates (2015)
  • Author(s): K. Yoshida, T. Yamaguchi, T. Takekiyo, Y. Yoshimura
  • Organizer: EMLG-JMLG annual meeting 2015
  • Place of Presentation: Rostock
  • Year and Date: 2015-09-06
  • Int'l Joint Research
• [Presentation] X線回折による水和ペプチドおよびタンパク質の水和構造 (2015)
  • Author(s): 吉田亨次, 山口敏男
  • Organizer: 日本化学会第９５春季年会
  • Place of Presentation: 日本大学
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 不凍タンパク質III型の水和水の構造とダイナミクス (2014)
  • Author(s): 吉田亨次，山口敏男，Giovanna Simeoni，Marie-Claire Bellissent-Funel
  • Organizer: 第３７回溶液化学シンポジウム
  • Place of Presentation: 佐賀大学
  • Year and Date: 2014-11-12 – 2014-11-14
• [Presentation] Thermal property, structure, and dynamics of hydrated proteins (2014)
  • Author(s): Koji Yoshida, Toshio Yamaguchi
  • Organizer: International Meeting on Applications of Statistical Mechanics of Molecular Liquid on Soft Matter
  • Place of Presentation: カセサート大学
  • Year and Date: 2014-09-14 – 2014-09-17
  • Invited
• [Presentation] Structure and dynamics of hydrated proteins revealed by X-ray scattering (2013)
  • Author(s): K. Yoshida, T. Yamaguchi
  • Organizer: EMLG - JMLG annual meeting 2013
  • Place of Presentation: リール(フランス)
• [Presentation] Terahertz dynamics and structure of hydrated protein studied by X-ray scattering (2013)
  • Author(s): K. Yoshida, T. Yamaguchi
  • Organizer: 33rd International Conference on Solution Chemistry
  • Place of Presentation: 京都
• [Presentation] 中性子散乱によるメソ細孔物質内の水のダイナミクス測定 (2013)
  • Author(s): 吉田亨次
  • Organizer: 日本化学会中国四国支部大会
  • Place of Presentation: 広島
  • Invited
• [Book] 分散系の測定・評価と設計・応用 (2016)
  • Author(s): 竹清貴浩，吉村幸浩，吉田亨次，山口敏男
  • Total Pages: 800
  • Publisher: テクノシステム