Sensitivity improvement of Elliptically-polarization-detected CD spectroscopy for the drug-biomolecule binidng study
Project/Area Number |
25410167
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Bio-related chemistry
|
Research Institution | Tohoku University |
Principal Investigator |
ARAKI Yasuyuki 東北大学, 多元物質科学研究所, 准教授 (80361179)
|
Co-Investigator(Renkei-kenkyūsha) |
WADA Takehiko 東北大学, 多元物質科学研究所, 教授 (20220957)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | キラリティ / 円二色性 / 高感度検出 / 微弱光検出 / フォトンカウンティング |
Outline of Final Research Achievements |
Cationic porphyrins are well-known as small molecules bound to double strand DNA (dsDNA) and also known as typical reagents of the photodynamic therapy. Recently, we reported new methodology for high sensitive circular dichroism (CD) spectroscopy and application for the analysis of the interaction dynamics of cationic porphyrins with dsDNA. In this study, we demonstrated the variety of cationic porphyrin derivatives with dsDNAs formed the intercalating complexes. Our time resolved CD measurement system revealed that the photo-irradiation of porphyrins intercalated to dsDNA showed the photo-bleaching, those were the originated from the photo-excited triplet state generating inside dsDNA. Transient absorption study for these porphyrin-dsDNA complexes were also consistent with these transient CD data. Thus we have concluded that we have successfully observed the photo-dynamics of porphyrin-dsDNA complexes by using our transient CD technique.
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Report
(4 results)
Research Products
(21 results)