| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Misfolded glycoproteins are toxic to the cell and are thought to be degraded by ubiquitin-proteasome system (UPS), however, the detail of the degradation pathway is not clearly known. In this study, I accomplished the total synthesis of ubiquitinated glycoprotein, and used it as a probe for the study of the degradation of glycoprotein by UPS. This is one of the very few example of the total synthesis of posttranslationally modified complex protein having oligosaccharide and ubiquitin. The degradation assay suggested that the newly developed synthetic ubiquitinated glycoprotein probe can be a valuable tool for the analysis of the degradation of glycoproteins by UPS.
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