Development of intracellular delivery method of proteins by hetero-dimerized leucine zippers
| Project/Area Number |
25410181
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kinki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MICHIUE Hiroyuki 岡山大学, 大学院医歯薬学総合研究科, 助教 (20572499)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ペプチド / DDS / 細胞 / ロイシンジッパー / 細胞内運搬ペプチド / p53タンパク質 / Nanogタンパク質 / ペプチド核酸 / p53 / Nanog / Beclin-1 |
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| Outline of Final Research Achievements |
For purpose of treatment of disease, we develop a method for delivering a functional proteins or functional peptides into cells. In this work, we synthesized an autophagy-inducing peptide (Beclin 1) modified with a hetero-dimerization leucine zipper peptide (LzK). We also synthesized a cell-penetrating peptide modified with a pair of LzK (LzE). When these peptides were mixed, Beclin 1 was successfully delivered into the cell, and the peptide induced autophagy.
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Report
(4 results)
Research Products
(5 results)
