Investigation of titanium peroxide nanoparticles for cancer therapy application

• Project/Area Number: 25420831
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kobe University
• Principal Investigator: OGINO Chiaki 神戸大学, 工学(系)研究科(研究院), 准教授 (00313693)
• Co-Investigator(Kenkyū-buntansha): SASAKI Ryouhei 神戸大学, 医学(系)研究科(研究院), 教授 (30346267)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 放射線照射 / チタン酸化物 / 活性酸素種 / ナノ粒子 / がん治療 / 二酸化チタン / 過酸化チタン / がん細胞損傷 / 過酸化水素

Outline of Final Research Achievements

Titanium peroxide nanoparticles (TiOxNPs) was synthesized and their efficacy as novel agents that can potently enhance the effects of radiation in the treatment of pancreatic cancer was investigated. The core structures of the PAA-TiOxNPs were found to be of the anatase type. The TiOxNPs and PAA-TiOxNPs showed a distinct ability to produce hydroxyl radicals in response to X-ray irradiation in a dose- and concentration-dependent manner, whereas the TiO2NPs did not. The combination of the PAATiOxNPs and X-ray irradiation induced significantly stronger tumor growth inhibition compared to treatment with either PAA-TiOxNPs or X-ray alone. In addition, bio-distribution of PAATiOx in Xenograft mouse model was identified, and the evaluation method of effect of X-ray irradiation on cell line was also established. Together these findings, TiOxNPs are potential agents for enhancing the effects of radiation on pancreatic cancer via hydroxyl radical production.

Research Products

• [Journal Article] Mutation of arginine residues to avoid non-specific cellular uptakes for hepatitis B virus core particles. (2015)
  • Author(s): Mohamed suffian, I.F., Nishimura, Y., Morita,K., Nakamura-Tsuruta, S., Al-Jamal, K.T., Ishii, J., Ogino, C., Kondo, A.
  • Journal Title: Journal of Nanobiotechnology Volume: in press Issue: 1 Pages: 0-0
  • DOI: 10.1186/s12951-015-0074-8
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a bio-nanocapsule via an endocytic uptake pathway. (2014)
  • Author(s): Nishimura, Y., Takeda, K., Ezawa, R., Ishii, J., Ogino, C., Kondo, A.
  • Journal Title: Journal of Nanobiotechnology Volume: 21(1) Issue: 1 Pages: 11-11
  • DOI: 10.1186/1477-3155-12-11
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Advances in the engineering of bio-nanoparticles for drug delivery. (2014)
  • Author(s): Nishimura, Y., Ishii, J., Ogino, C., Kondo, A.
  • Journal Title: Journal of Biomedical Nanotechnology Volume: 10(9) Issue: 9 Pages: 2063-2085
  • DOI: 10.1166/jbn.2014.1951
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Granting specificity for breast cancer cells using a Hepatitis B core particle with a HER2-targeted affibody molecule (2013)
  • Author(s): Y. Nishimura, W. Mimura, I.F. Mohamed suffian, T. Amino, J. Ishii, C. Ogino, A. Kondo
  • Journal Title: J. Biochem. Volume: 153(3) Pages: 251-256
  • Peer Reviewed
• [Journal Article] An affinity chromatography method used to purify His-tag-displaying bio-nanocapsules (2013)
  • Author(s): . Nishimura, K. Takeda, J. Ishii, C. Ogino, A. Kondo
  • Journal Title: J. Virol. Methods Volume: 189(2) Pages: 393-396
  • Peer Reviewed
• [Journal Article] argeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes (2013)
  • Author(s): Y. Nishimura, H. Mieda, J. Ishii, C. Ogino, T. Fujiwara, A. Kondo
  • Journal Title: J. Nanobiotechnology Volume: 11 Pages: 19-19
  • Peer Reviewed
• [Presentation] Development of novel radiosensitizing cancer therapy: Combination of radiotherapy and titanium peroxide nanoparticle (2015)
  • Author(s): K. Morita；T. Suzuki；M. Nakayama；R. Sasaki；K. Sato；C. NUMAKO；C. Ogino；A. Kondo
  • Organizer: Pachifichem2015
  • Place of Presentation: Hawaii Convention Center
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Characterization for metal-oxide nanoparticles generating radicals with X-ray irradiation (2015)
  • Author(s): C. NUMAKO；S. Miyazaki；K. Sato；S. Takami；C. Ogino；A. Kondo
  • Organizer: Pachifichem2015
  • Place of Presentation: Hawaii Convention Center
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] 担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布特性の調査 (2015)
  • Author(s): 鈴木 貴弘； 森田 健太； 西村 勇哉； 荻野 千秋； 近藤 昭彦
  • Organizer: 化学工学会第47回秋季大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2015-09-09
• [Presentation] 放射線治療と相乗効果を有するポリアクリル酸修飾過酸化チタンナノ粒子の作用機序解明 (2015)
  • Author(s): 森田 健太； 鈴木 貴弘； 西村 勇哉；沼子 千弥；佐藤 和好；中山 雅央； 佐々木 良平；荻野 千秋；近藤 昭彦
  • Organizer: 化学工学会第47回秋季大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2015-09-09
• [Presentation] 担癌マウスへのTiナノ粒子投与による生体内分布 (2015)
  • Author(s): 鈴木貴弘、田野亜都里、森田健太、荻野千秋、近藤昭彦
  • Organizer: 第17回化学工学会学生発表
  • Place of Presentation: 徳島大学常三島キャンパス
  • Year and Date: 2015-03-07
• [Presentation] 放射性がん治療との併用に向けたか酸化チタンナノ粒子の物性評価 (2014)
  • Author(s): 森田健太、田野亜都里、鈴木貴弘、沼子千弥、宮嵜世里加、佐藤和好、仲山雅央、佐々木良平、荻野千秋、近藤昭彦
  • Organizer: 生物工学若手研究者の集い夏のセミナー
  • Place of Presentation: 神戸セミナーハウス
  • Year and Date: 2014-07-12 – 2014-07-13
• [Presentation] 上皮成長因子受容体を特異的に認識するAffibody提示バイオナノカプセルの開発 (2014)
  • Author(s): 江澤僚将、西村勇哉、石井純、荻野千秋、近藤昭彦
  • Organizer: 生物工学若手研究者の集い夏のセミナー
  • Place of Presentation: 神戸セミナーハウス
  • Year and Date: 2014-07-12 – 2014-07-13
• [Presentation] 新しい放射線治療をめざした過酸化チタンナノ粒子の物性研究 (2014)
  • Author(s): 森田健太、田野亜都里、沼子千弥、佐藤和好、中山雅央、佐々木良平、荻野千秋、近藤昭彦
  • Organizer: 化学工学会第79年会
  • Place of Presentation: 岐阜大学
• [Presentation] 上皮成長因子受容体を特異的に認識するAffibody 提示バイオナノカプセルの開発 (2013)
  • Author(s): 江澤僚将、西村勇哉、石井純、荻野千秋、近藤昭彦
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場
• [Presentation] ナノ粒子とX 線照射を併用した深部ガンの非侵襲的治療法の開発 (2013)
  • Author(s): 田野亜都里、森田健太、荻野千秋、佐藤和好、沼子千弥、中山雅央、佐々木良平、近藤昭彦
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場