| Project/Area Number |
25430010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI HIROO 奈良県立医科大学, 医学部, 助教 (20390685)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUBOI AKIO 奈良県立医科大学, 医学部, 教授 (20163868)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 成体神経新生 / 嗅球介在ニューロン / シナプス形成 / 転写因子 / 嗅球 / Npas4 / 神経活動依存的な発達 / スパイン / 介在ニューロン |
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| Outline of Final Research Achievements |
Sensory experience regulates development in olfactory bulb (OB). Recently, we identified a transcription factor, neuronal Per/Arnt/Sim domain protein 4 (Npas4) gene, which is expressed in a subset of OB GCs following sensory experience. Npas4 overexpression in newborn OB GCs increased the spine density even under sensory deprivation. Conversely, both Npas4 knockdown and knockout resulted in a significant reduction in the spine density of OB GCs. Then, to investigate molecules that play a role in the downstream of Npas4, we searched for microRNAs (miRs), whose expression levels differed between the wild-type and Npas4-knockout OBs, and whose expression correlated with the interneurons, based on RNA-sequencing plus in situ hybridization screenings. Among ~50 miRs, we found that three novel miRs show a clear difference in expression between the wild-type and Npas4-knockout OBs.
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