Generation of the network modified mouse with disruption of amygdala-BNST connections

• Project/Area Number: 25430031
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: Suto Fumikazu 国立研究開発法人国立精神・神経医療研究センター, 神経研究所 微細構造研究部, 室長 (40345848)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 神経科学 / 分子 / 発生・再生 / 神経回路形成 / 扁桃体 / 軸索誘導分子 / プレキシン / セマフォリン / 神経 / 行動 / 軸索誘導 / 発生・発達・再生神経科学 / 発生生物学 / 神経発生学 / 分子遺伝学 / 神経行動学 / 分子生物学 / 発生 / 発達 / 情動行動 / 哺乳行動

Outline of Final Research Achievements

The amygdaloid network is a principal component of the limbic system with roles in emotion, feeding and sexual behavior. Axons of distinct amygdaloid　nuclei connect to the bed nucleus of the stria terminalis (BNST) in a topographic manner.　However, it remains largely unknown the roles　or contributions of this topographic connections for animal behavior. We have tried to generate the network modification animal restricted in amygdala-BNST connections. We generated the conditional targeting vectors for three axon guidance genes (plexin-A4, Sema6A. Sema6B), regulating the formation of the topographic connection, and established two ES cell lines with targeted recombination for plexin-A4.

Research Products

• [Journal Article] lexinA4-dependent retrograde Semaphorin3A signaling regulates the dendritic localization of GluA2-containing AMPA receptors. (2014)
  • Author(s): 1) Yamashita, N., Usui, H., Nakamura, F., Chen, S., Sasaki, Y., Hida, T., Suto, F., Taniguchi, M., Takei, K., Goshima, Y.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 3424-3424
  • DOI: 10.1038/ncomms4424
  • Peer Reviewed / Open Access
• [Presentation] Plexin-A4 Mediates the Maintenance of Postnatal Callosal Axons. (2014)
  • Author(s): Son A, Suto F, Morozov Y, Ishii S, Rakic P, Levitt P, Hashimoto-Torii K, Torii M
  • Organizer: Neuroscience 2014, 34th Annual Meeting of SfN
  • Place of Presentation: Walter E. Washington Convention Center, Washington DC, アメリカ合衆国
  • Year and Date: 2014-11-18
• [Presentation] Semaphorin/plexin signaling regulates the development of the amygdala-BNST network． (2014)
  • Author(s): Suto F, Kawaguchi M, Osumi N, Ichinohe N
  • Organizer: 第47回日本発生生物学会年会
  • Place of Presentation: WINC AICHI 愛知県産業労働センター、名古屋、愛知
  • Year and Date: 2014-05-27 – 2014-05-28