Roles of mDia, an actin nucleation factor, in cell transformation
| Project/Area Number |
25430109
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Rho / mDia / 癌化 / Ras / アクチン細胞骨格 / 細胞悪性化 / ERK / TPA / 足場非依存性増殖 / 細胞骨格 / アクチン / 化学発癌 |
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| Outline of Final Research Achievements |
It has been reported that Rho signaling is involved in several cellular processes such as cell migration and cell adhesion. In cancer cells, Rho regulates cancer metastasis and tumorigenesis. However, its underlying molecular mechanism is still unknown. In this study, we examined whether mDia, which is a downstream protein of Rho, is involved in Ras-dependent tumorigenesis utilizing DMBA/TPA two-stage chemical carcinogenesis model in mice and examined activation of Ras downstream proteins such as Raf-MEK-ERK and AKT in mDia deleted cultured cells. In conclusion, it was found that the number of papilloma was significantly decreased in mDia1 KO mice. Moreover we found depletion of mDia1 caused to the suppression of MEK-ERK pathway in cultured cells. These results indicate that Rho-mDia signaling plays an important role in Ras-dependent tumorigenesis through MEK-ERK activation.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a2016
Author(s)
Yamaoka M, Ando T, Terabayashi T, Okamoto M, Takei M, Nishioka T, Kaibuchi K, Matsunaga K, Ishizaki R, Izumi T, Niki I, Ishizaki T, Kimura T
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Journal Title
Journal of Cell Science
Volume: 129
Issue: 3
Pages: 637-649
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Loss of a Rho-regulated actin nucleator, mDia2, impairs cytokinesis during mouse fetal erythropoiesis.2013
Author(s)
Watanabe S, De Zan T, Ishizaki T, Yasuda S, Kamijo H, Yamada D, Aoki T, Kiyonari H, Kaneko H, Shimizu R, Yamamoto M, Goshima G, Narumiya S.
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Journal Title
Cell Report
Volume: 5
Pages: 926-932
NAID
Related Report
Peer Reviewed
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[Journal Article] Activated Cdc42-bound-IQGAP1 determines the cellular endocytic site2013
Author(s)
Kimura T, Yamaoka M, Taniguchi S, Okamoto M, Takei M, Ando T, Iwamatsu A, Watanabe T, Kaibuchi K, Ishizaki T, Niki I
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Journal Title
Mol. Cell. Biol
Volume: 33
Issue: 24
Pages: 4834-43
DOI
Related Report
Peer Reviewed
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[Presentation] Identification of Rab27a-GAP-interacting proteins and its functional analysis in pancreatic beta-cells.2014
Author(s)
Yamaoka M, Ando T, Okamoto M, Terabayashi T, Matsunaga K, Ishizaki R, Izumi T, Niki I, Ishizaki T, Kimura T
Organizer
50th Annual meeting of European Association for the Study of Diabetes
Place of Presentation
Reed Messe Vienna, Vienna, Austria
Year and Date
2014-09-15 – 2014-09-19
Related Report
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[Presentation] Endogenous hydrogen sulfide protects pancreatic beta-cells from a high-fat diet-induced glucotoxicity and prevents the development of type 2 diabetes.2014
Author(s)
Okamoto M, Yamaoka M, Ando T, Takei M, Taniguchi S, Terabayashi T, Niki I, Ishizaki T, Kimura T
Organizer
3rd International Conference on Hydrogen Sulfide in Biology and Medicine
Place of Presentation
Kyoto University, Kyoto, Japan
Year and Date
2014-06-04 – 2014-06-06
Related Report
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