Crystallographic analysis of a proton transporting protein at high resolution

• Project/Area Number: 25440020
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: Takeda Kazuki 京都大学, 理学(系)研究科(研究院), 准教授 (30332290)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: タンパク質 / 結晶 / 界面活性剤

Outline of Final Research Achievements

roton transport is mediated my membrane proteins involved in photosynthesis, electron transport, substrate transport and so on. Therefore, it is one of the most important processes in the biological system. I performed a high resolution crystallographic analysis of bacteriorhodopsin, which is the most studied proton transporting protein. According to the optimization of conditions for the crystallization and data collection, we succeeded in collecting a high resolution data set without X-ray damages. Respective non-hydrogen atoms are clearly observed on the electron density map. In addition, electron densities for some hydrogen atoms are observed in the analysis.