| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Outline of Final Research Achievements |
GroEL is able to use both the “bullet cycle” and the “football cycle”, the selection will depend on the situation in the cell. We determined the crystal structure of the “football” GroEL:GroES2 complex. The overall structure of the “football” complex resembled the GroES-bound GroEL-ring of the asymmetric 1:1 GroEL:GroES complex (the “bullet” complex). However, the two GroES-bound GroEL-rings form a modified interface by a rotation about the 7-fold axis. As a result, the inter-ring contacts between the two GroEL rings in the football complex differed from those in the bullet complex. The altered inter-GroEL ring contacts in the football structure lead GroEL tetradecamer to dissociated in the presence of ATP and GroES in a native gel.
Furthermore, we constructed coexpression system of distinct fluorescence molecules-labeled GroES in E. coli for the purpose of detection of the football-like reaction intermediates by FCCS.
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