| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Using starfish oocytes, we found that cyclin B mRNAs with short poly(A) tails were uridylated and that hormonal stimulation induced deuridylation and poly(A) elongation. When we injected synthetic RNAs of cyclin B into oocytes prior to hormonal stimulation, uridylated RNAs were as stable as non-uridylated RNAs. Following hormonal stimulation, the 3’ termini of short poly(A) tails of synthesized RNAs containing oligo(U) tails were trimmed, and their poly(A) tails were subsequently elongated. These results indicate that uridylation in cyclin B mRNA of starfish oocytes does not mediate mRNA decay; instead, hormonal stimulation induces partial degradation of uridylated short poly(A) tails in the 3’ to 5’ direction, followed by poly(A) elongation. Oligo(U) tails may be involved in translational inactivation of mRNAs.
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