Mechanism of maternal mRNA stored in oocytes

• Project/Area Number: 25440102
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Ochanomizu University
• Principal Investigator: Chiba Kazuyoshi お茶の水女子大学, 基幹研究院, 教授 (70222130)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: mRNA / ウリジル化 / サイクリンB / 卵母細胞 / 母性RNA / ホルモン / ポリA / ポリＡ鎖 / サイクリンＢ / ポリＡ / ポリＵ / 減数分裂 / 卵簿細胞 / ヒトデ

Outline of Final Research Achievements

Using starfish oocytes, we found that cyclin B mRNAs with short poly(A) tails were uridylated and that hormonal stimulation induced deuridylation and poly(A) elongation. When we injected synthetic RNAs of cyclin B into oocytes prior to hormonal stimulation, uridylated RNAs were as stable as non-uridylated RNAs. Following hormonal stimulation, the 3’ termini of short poly(A) tails of synthesized RNAs containing oligo(U) tails were trimmed, and their poly(A) tails were subsequently elongated. These results indicate that uridylation in cyclin B mRNA of starfish oocytes does not mediate mRNA decay; instead, hormonal stimulation induces partial degradation of uridylated short poly(A) tails in the 3’ to 5’ direction, followed by poly(A) elongation. Oligo(U) tails may be involved in translational inactivation of mRNAs.

Research Products

• [Journal Article] Hormonal stimulation of starfish oocytes induces partial degradation of the 3′ termini of cyclin B mRNAs with oligo(U) tails, followed by poly(A) elongation (2016)
  • Author(s): Hiroe Ochi and Kazuyoshi Chiba
  • Journal Title: RNA Volume: 22 Issue: 6 Pages: 1-8
  • DOI: 10.1261/rna.054882.115
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Block of CDK1-dependent polyadenosine elongation of Cyclin B mRNA in metaphase I-arrested starfish oocytes is released by intracellular pH elevation upon spawning (2016)
  • Author(s): Hiroe Ochi, Saki Aoto, Kazunori Tachibana, Masatoshi Hara and Kazuyoshi Chiba
  • Journal Title: Molecular Reproduction and Development Volume: 83 Issue: 1 Pages: 79-87
  • DOI: 10.1002/mrd.22599
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sperm from sneaker male squids exhibit chemotactic swarming to CO_2 (2013)
  • Author(s): Hirohashi N, Alvarez L, Shiba K, Fujiwara E, Iwata Y, Mohri T, Inaba K, Chiba K, Ochi H, Supuran CT, Kotzur N, Kakiuchi Y, Kaupp UB, Baba SA
  • Journal Title: Current Biology Volume: 23 Issue: 9 Pages: 775-781
  • DOI: 10.1016/j.cub.2013.03.040
  • Peer Reviewed
• [Journal Article] Arrest at Metaphase of Meiosis I in Starfish Oocytes in the Ovary is Maintained by High CO2 and Low O2 Concentrations in Extracellular Fluid (2013)
  • Author(s): Moriwaki,K., Nakagawa,T., Nakaya,F., Hirohashi,N., and Chiba,K
  • Journal Title: Zool Sci Volume: 30 Issue: 11 Pages: 975-984
  • DOI: 10.2108/zsj.30.975
  • Peer Reviewed
• [Presentation] ヒトデ卵減数分裂進行時におけるcyclinB mRNAのオリゴウリジレーションとポリA鎖伸長 (2015)
  • Author(s): 越智洋絵, 千葉和義
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 新潟コンベンションセンタ（新潟・中央区）
  • Year and Date: 2015-09-17
• [Presentation] Ability of paternal asters to form meiotic spindle during oocyte maturation in starfish (2015)
  • Author(s): CHIBA Kazuyoshi
  • Organizer: Oocyte Maturation and Fertilization Meeting IV
  • Place of Presentation: 浅虫海洋生物学教育研究センター（青森・浅虫）
  • Year and Date: 2015-06-15
  • Int'l Joint Research
• [Presentation] Removal of 3' terminal oligo(U) tail followed by poly(A) elongation of cyclin B mRNA in starfish oocytes resuming meiosis (2015)
  • Author(s): OCHI Hiroe, CHIBA Kazuyoshi
  • Organizer: Oocyte Maturation and Fertilization Meeting IV
  • Place of Presentation: 浅虫海洋生物学教育研究センター（青森・浅虫）
  • Year and Date: 2015-06-15
  • Int'l Joint Research
• [Presentation] ヒトデ卵母細胞の減数分裂進行時におけるmRNAポリA鎖伸長調節 (2014)
  • Author(s): 越智洋絵 原昌稔 立花和則 青砥早希 千葉和義
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] イトマキヒトデ未受精卵のアポトーシスにおける Apaf-1 CARDによるcaspase-3/9の活性化機構 (2014)
  • Author(s): 田村 りつ子、高田 真理子 、吉田 綾香、千葉 和義
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Meiotic arrest and fertilization of starfish oocytes (2013)
  • Author(s): Kazuyoshi Chiba
  • Organizer: The EMBO workshop “Oocyte maturation and fertilization meeting: Lessons from canonical and emerging models”
  • Place of Presentation: Banyuls-sur-Mer, France
  • Invited