| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
The main purpose of this study is to show the evidence that output phenotypes are variously changed in timing-dependent manner of ERK activation in early developmental stages in vertebrates. Phenotypical effects by microinjection of several mRNAs or DNAs into Xenopus embryos showed that obvious turning points of ERK effects were existed at pre-blastula, mid-blastula, and gastrula stages. Incidentally, new DNA constructs to activate ERK by exposure of the reagent Rapamycin or by heat treatment were also created in this study. They should be adaptable for observation of ERK activation effects in later stages by transgenic assay and for various cell biological assays.
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