Functional analysis of a gene that is involved in seed aleurone and epidermis development

• Project/Area Number: 25440142
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Meiji University
• Principal Investigator: Kawakami Naoto 明治大学, 農学部, 教授 (10211179)
• Co-Investigator(Renkei-kenkyūsha): Toyooka Kiminori 理化学研究所, 環境資源科学研究センター, 上級研究員 (10360596)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 種子 / 胚乳 / 糊粉層 / 発芽 / 小胞輸送 / 細胞分化 / 植物生理学 / 形態形成 / 表皮 / クラスリン被覆小胞

Outline of Final Research Achievements

Outermost cell layer of endosperm, aleurone, works for mobilization of storage materials in cereal grains. In contrast, aleurone works mainly as a barrier of germination in dicot seeds. In this study, we found TRG2A gene as an important factor for aleurone layer development and/or maintenance by using Arabiodpsis mutant that lost all the endosperm cells during seed development. TRG2A protein was found to be localized in cytoplasm and interact with adaptin subunits. We found that CHMP1 is also involved in aleurone development in Arabidopsis. Our findings indicate that vesicle transport system has critical role on aleurone development.

Research Products

• [Journal Article] ABA-INSENSITIVE3, ABA-INSENSITIVE5, and DELLAs Interact to Activate the Expression of SOMNUS and Other High-Temperature-Inducible Genes in Imbibed Seeds in Arabidopsis (2013)
  • Author(s): Soohwan Lim, Jeongmoo Park, Nayoung Lee, Jinkil Jeong, Shigeo Toh, Asuka Watanabe, Junghyun Kim, Hyojin Kang, Dong Hwan Kim, Naoto Kawakami, and Giltsu Choi
  • Journal Title: The Plant Cell Volume: 25 Issue: 12 Pages: 4863-4878
  • DOI: 10.1105/tpc.113.118604
  • Peer Reviewed
• [Presentation] Functional analysis of TRG2A gene which is involved in the development of endosperm aleurone in Arabidopsis seed (2016)
  • Author(s): Yuki Uchida, Kazutaka Akahori, Reo Sugiyama, Yukinori Yoshino, Michio Sato, Kiminori Toyooka, Naoto Kawakami
  • Organizer: 5th Workshop on the Molecular Aspects of Seed Dormancy and Germination
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2016-05-31
  • Int'l Joint Research
• [Presentation] 種子の糊粉層の形成・維持に関わるシロイヌナズナのTRG2Aタンパク質の解析 (2016)
  • Author(s): 赤堀 一貴，杉山 礼央，内田 悠稀，吉野 幸則，佐藤 道夫，豊岡 公徳，川上 直人
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手
  • Year and Date: 2016-03-18
• [Presentation] アダプチン結合領域を持つTRG2タンパク質の機能解析 (2015)
  • Author(s): 杉山礼央、吉野幸則、赤堀一貴、内田悠稀、川上直人
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-07
• [Presentation] 胚乳最外層の消失と植物体の形態異常をもたらすシロイヌナズナtrg2突然変異の解析 (2015)
  • Author(s): 吉野幸則、赤堀一貴、杉山礼央、鴨志田葵、内田悠輝、田中新太、五味渕苑子、佐藤道夫、川上直人
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-17
• [Presentation] Analysis of Arabidopsis thermoinhibition resistant germination 2 that has defect in aleurone layer and epidermis (2014)
  • Author(s): Yukinori Yoshino, Arata Tanaka, Sonoko Gomibuchi, Kazutaka Akahori, Reo Sugiyama, Aoi Kamoshita and Naoto Kawakami
  • Organizer: XII France-Japan Workshop on Plant Science 2014 -Plant Responses to Environment
  • Place of Presentation: 東京
  • Year and Date: 2014-10-27 – 2014-10-29
• [Presentation] 種子の糊粉層形成に異常を持つシロイヌナズナの突然変異体trg2の解析 (2014)
  • Author(s): 赤堀一貴、吉野幸則、五味渕苑子、杉山礼央、鴨志田葵、川上直人
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 川崎
  • Year and Date: 2014-09-13
• [Presentation] Identification of TRG2 as a novel gene that is involved in development and/or maintenance of aleurone layer cells of Arabidopsis seeds (2013)
  • Author(s): Sonoko Gomibuchi, Kazutaka Akahori, Arata Tanaka and Naoto Kawakami
  • Organizer: 4th Workshop on the Molecular Aspects of Seed Dormancy and Germination
  • Place of Presentation: パリ
• [Presentation] 種子の糊粉層形成に異常を持つシロイヌナズナの突然変異 体trg2 の解析
  • Author(s): 吉野幸則、五味渕苑子、赤堀一貴、笹本真語、田中新太、山下結、川上直人
  • Organizer: 第34回 種子生理生化学研究会年会
  • Place of Presentation: 箱根
• [Presentation] 種子糊粉層の消失をもたらすシロイヌナズナtrg2突然変異遺伝子の解析
  • Author(s): 吉野 幸則，五味渕 苑子，赤堀 一貴，杉山 礼央，鴨志田 葵，川上 直人
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Remarks] 教員データベース
  • URL: http://gyoseki1.mind.meiji.ac.jp/mjuhp/KgApp?kyoinId=ymiygyymggy