Revealing regulation mechanisms of organeller genomes to environmental change in the coral symbionts, Symbiodinium spp.

• Project/Area Number: 25440182
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Okinawa Institute of Science and Technology Graduate University
• Principal Investigator: SHOGUCHI Eiichi 沖縄科学技術大学院大学, その他の研究科, 研究員 (90378563)
• Co-Investigator(Renkei-kenkyūsha): MUNGPAKDEE Sutada 沖縄科学技術大学院大学, マリンゲノミックスユニット, 研究員 (10623647)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: オルガネラゲノム / 渦鞭毛藻 / Symbiodinium / RNA編集 / ノンコーディングRNA / 共生 / サンゴ / 進化 / プラスチドゲノム / ミトコンドリアゲノム / RNAエディティング / ノンコーディング RNA / 環境応答 / ミニサークル / 発現調節

Outline of Final Research Achievements

The plastid and mitochondrial genomes were decoded and analyzed in the symbiotic dinoflagellate, Symbiodinium minutum. The papers showing these results were published (Mungpakdee et al., 2014; Shoguchi et al., 2015). The structures of plastid genome were fourteen minicircle DNA (1.8-3.3 kbp). Each circle encodes one gene, which was edited in RNA processing. The mitochondrial genome analysis reveals the conservation of non-coding RNAs between Symbiodinium and the apicomplexan, Plasmodium falciparum.

Research Products

• [Journal Article] Multifunctional polyketide synthase genes identified by genomic survey of the symbiotic dinoflagellate, Symbiodinium minutum (2015)
  • Author(s): Beedessee, G., Hisata, K., Roy, M.C., Satoh, N., Shoguchi, E.
  • Journal Title: BMC Genomics Volume: 16 Issue: 1 Pages: 941-941
  • DOI: 10.1186/s12864-015-2195-8
  • Peer Reviewed / Open Access
• [Journal Article] The large mitochondrial genome of Symbiodinium minutum reveals conserved non-coding sequences between dinoflagellates and apicomplexans (2015)
  • Author(s): Shoguchi, E., Shinzato, C., Hisata, K., Satoh, N., Mungpakdee, S.
  • Journal Title: Genome Biol. Evol. Volume: 7 Issue: 8 Pages: 2237-2244
  • DOI: 10.1093/gbe/evv137
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome. (2014)
  • Author(s): Mungpakdee, S., Shinzato, C., Takeuchi, T., Kawashima, T., Koyanagi, R., Hisata, K., Tanaka, M., Goto, H., Fujie, M., Lin, S., Satoh, N., and Shoguchi, E.
  • Journal Title: Genome Biol Evol. Volume: 6 Issue: 6 Pages: 1408-1422
  • DOI: 10.1093/gbe/evu109
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 褐虫藻３種のゲノムとその多様性 (2016)
  • Author(s): 將口栄一, 新里宙也, 久田香奈子, 佐藤矩行
  • Organizer: 日本藻類学会
  • Place of Presentation: 日本歯科大学, 東京
  • Year and Date: 2016-03-20
• [Presentation] Comparative genomics of symbiotic marine dinoflagellates (2015)
  • Author(s): Shoguchi, E., Shinzato, C., Hisata, K., Satoh, N.
  • Organizer: Academia Sinica-OIST Joint Meeting “Evolution of Complex Systems”
  • Place of Presentation: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan
  • Year and Date: 2015-12-16
• [Presentation] Comparative genomics of marine symbiotic dinoflagellates (2015)
  • Author(s): Shoguchi, E.
  • Organizer: 2015 international symposium on marine genomics
  • Place of Presentation: The palace hotel soul, Korea
  • Year and Date: 2015-06-23
  • Invited
• [Presentation] 褐虫藻Symbiodinium minutumのミトコンドリアゲノムの解読 (2015)
  • Author(s): 將口栄一，新里宙也，久田香奈子，佐藤矩行
  • Organizer: 日本藻類学会
  • Place of Presentation: 九州大学箱崎キャンパス
  • Year and Date: 2015-03-20 – 2015-03-22
• [Presentation] 褐虫藻類のゲノムの解読 (2013)
  • Author(s): 將口栄一、新里宙也、川島武士、佐藤矩行
  • Organizer: 日本サンゴ礁学会
  • Place of Presentation: 沖縄科学技術大学院大学