Establishment of highly efficient chloroplast genome editing by using synthetic custom design nucleases
| Project/Area Number |
25450002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science in genetics and breeding
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| Research Institution | The University of Tokushima |
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Principal Investigator |
Osakabe Keishi 徳島大学, 農工商連携センター, 特任教授 (70450335)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 葉緑体 / ゲノム編集 / TALEN / Cas9 / タバコ / CRISPR / CRISPR/CAS9 / 遺伝子ターゲッティング |
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| Outline of Final Research Achievements |
To establish chloroplast genome editing in order to open a frontier of chloroplast engineering, site-specific DNA cleavage system by using the synthetic custom design nuclease was designed and constructed. At first, TALEN as a synthetic custom design nuclease was chosen, and The TALEN with transit peptide (tp-TALEN) against to the target on chloroplast genome was constructed. The tp-TALEN could cut the target DNA specifically in vitro, but was failed to transport to chloroplast. Therefore, Cas9 nuclease was used instead of TALEN. Cas9 with transit peptide was successively transported to chloroplast.
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Report
(4 results)
Research Products
(12 results)
