| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Using affinity purification and mass spectrometry, we have identified a host protein GAPDH-A from Nicotiana benthamiana plants as the interacting partner of the movement protein (MP) of Red clover necrotic mosaic virus (RCNMV). GAPDH-A colocalized with virus replication complex (VRC) of RCNMV on the endoplasmic reticulum. We revealed that GAPDH-A is the host factor protein that contributes to the viral cell-to-cell movement, through its function to recruit the MP to VRCs. Furthermore, we showed the cortical punctate structures that contain the MP and VRC are required for the viral cell-to-cell movement and are not required for the replication of viral genomic RNA.
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