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New development of non-isotope heavy metals as alternative electron stains of uranyl acetate for plant pathology samples

Research Project

Project/Area Number 25450059
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant protection science
Research InstitutionKobe University

Principal Investigator

Park Pyoyun  神戸大学, (連合)農学研究科(研究院), 名誉教授 (20147094)

Co-Investigator(Kenkyū-buntansha) IKEDA Kenichi  神戸大学, 大学院・農学研究科, 准教授 (40437504)
PARK Moon-Son  神戸大学, 大学院・農学研究科, 学術推進員 (50600417)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords酢酸ウラン / 透過電子顕微鏡 / 非放射性重金属剤 / 塩化ハフニウム / ガドリ二ウム塩 / サマリウム塩 / 細胞コントラスト / ネガティブ染色 / 非放射性重金属染色 / 酢酸ガドリニウム / サマリ二ウム塩 / 酢酸ガドリ二ウム / 塩化ガドリ二ウム / ネガテイブ染色 / 電子顕微鏡 / 塩化ハフニニうウム / 希土類
Outline of Final Research Achievements

Uranyl salts are effective to observe the cells of sections from plant pathology samples with much polysaccharides, but not to increase the contrast of cell walls, starch grains, glycogen particles and collagen fibers. Because of the radioactive salts, the use and purchase have been restricted only in Japan. The non-isotope heavy metals, positive to stain both the cell structures and the polysaccharides, are requested now. The staining effects of 5 different metals (SmCl3, SmAc3, GdCl3, GdAc3 and HfCl4) was examined ultrastructurally in cells of plant leaves and roots, insects, and fungi. Among them, GdAc3 and HfCl4 were excellent as positive electron stains whereas GdCl3 was good as a negative stain.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (28 results)

All 2016 2015 2014 2013

All Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 10 results,  Open Access: 8 results,  Acknowledgement Compliant: 3 results) Presentation (18 results) (of which Int'l Joint Research: 1 results,  Invited: 12 results)

  • [Journal Article] Suppression of chloroplastic alkenal/one oxidoreductase represses the carbon catabolic pathway in Arabidopsis thaliana leaves during night2016

    • Author(s)
      Takagi, Ifuku, Ikeda, Inoue, Park, Tamoi, Inoue, Sakamoto, Saito, Miyake
    • Journal Title

      Plant Physiology

      Volume: 170 Issue: 4 Pages: 2024-2039

    • DOI

      10.1104/pp.15.01572

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Novel aspects of hydrophobins in wheat isolate of Magnaporthe oryzae: Mpg1, but not Mhp1, is essential for adhesion and pathogenicity.2016

    • Author(s)
      Inoue, K., Kitaoka, H., Park, P., Ikeda, K.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 82 Issue: 1 Pages: 18-28

    • DOI

      10.1007/s10327-015-0632-9

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rapid contrast evaluation method based on affinity beads and backscattered electron imaging for the screening of electron stains.2015

    • Author(s)
      Kaku, H., Inoue, K., Muranaka, Y., Park, P., Ikeda, K.
    • Journal Title

      Microscopy

      Volume: 64 Issue: 5 Pages: 361-368

    • DOI

      10.1093/jmicro/dfv041

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Polycationic ruthenium red is reactive with the extracellular matrix produced by plant pathogenic fungi.2015

    • Author(s)
      Ueno, S., Inoue, K., Ishii, H., Jiang, S., Adachi, Y., Kanematsu, S., Park, P., Ikeda, K.
    • Journal Title

      Journal of Electron Microscopy Technology for Medicine and Biology

      Volume: 28 Pages: 47-53

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Accelerated senescence and enhanced disease resistance in hybrid chlorosis lines derived from interspecific crosses between tetraploid wheat and Aegilops tauschii2015

    • Author(s)
      Nakano, H.,Mizuno,N.,Tosa,Y., Yoshida,K., Park,P., Takumi,S.
    • Journal Title

      PLoS ONE

      Volume: 10

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Polycationic Ruthenium Red is Reactive with the Extracellular Matrix Produced by Plant Pathogenic Fungi2015

    • Author(s)
      Ueno, S., Inoue,K., Ishi, H., Jiang S., Adachi,Y., Kanematsu, A., Park,P., Ikeda, K.
    • Journal Title

      J. Electr. Microsc. Technol. Med. Biol.

      Volume: 28 Pages: 1-7

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Penetration behavior of Venturia nashicola, associated with H2O2 generation, in Asian and European Pear Leaves2014

    • Author(s)
      Jiang,S., Park, P. and Ishii, H.
    • Journal Title

      J.Phytopathology

      Volume: 162 Pages: 770-778

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Durable and broad-spectrum disease protection measure against airborne phytopathogenic fungi by using the detachment action of gelatinolytic bacteria2014

    • Author(s)
      Kitagawa, H., Shimoi, S., Inoue, K., Park, P., and Ikeda, K
    • Journal Title

      Biological Control

      Volume: 71 Pages: 1-6

    • DOI

      10.1016/j.biocontrol.2013.12.008

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Potentiation of mycovirus transmission by zinc compounds via attenuation of heterogenic incompatibility in Rosellinia necatrix.2013

    • Author(s)
      Ikeda, K., Inoue, K., Kida, C., Uwamori, T., Sasaki, A., Kanematsu, S., and Park, P.
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 79 Issue: 12 Pages: 3684-3691

    • DOI

      10.1128/aem.00426-13

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Appressorium-localized NADPH oxidase B is essential for aggressiveness and pathogenicity in the host-specific, toxin-producing fungus Alternaria alternata Japanese pear pathotype.2013

    • Author(s)
      Morita Y
    • Journal Title

      Mol Plant Pathol

      Volume: 14. Issue: 4 Pages: 365-378

    • DOI

      10.1111/mpp.12013

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 酸素バブリング・アルデヒド固定と重金属染色2016

    • Author(s)
      朴 杓允
    • Organizer
      第38回近畿電顕技術情報交換会
    • Place of Presentation
      神戸大学梅田インテリジェントラボラトリ教室、大阪
    • Year and Date
      2016-03-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 超薄切片形成機構と切片を取り巻く最新の電顕技法2016

    • Author(s)
      朴 杓允
    • Organizer
      第21回細胞構造研究会
    • Place of Presentation
      大阪大学コンベンションセンター、大阪
    • Year and Date
      2016-01-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Evaluation of non-cluclear uranium by comparison with continuous ultra-thin sections of biological samples.2015

    • Author(s)
      Muranaka,Y., Inoue, K., Park,P.
    • Organizer
      東アジア顕微鏡学会
    • Place of Presentation
      姫路
    • Year and Date
      2015-11-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 電子染色機序2015

    • Author(s)
      朴 杓允
    • Organizer
      植物電子顕微鏡サマーセミナー2015 (20th 細胞構造研究会@関東)、植物電子顕微鏡ネットワーク主催
    • Place of Presentation
      理研 横浜
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 定量形態学Stereologyの原理2015

    • Author(s)
      朴 杓允
    • Organizer
      第19回細胞構造研究会
    • Place of Presentation
      大阪大学コンベンションセンター  大阪
    • Year and Date
      2015-06-20
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 走査型電子顕微鏡を用いた代替電子染色剤の選抜法2015

    • Author(s)
      池田健一、嘉久大毅、井上加奈子、朴杓允
    • Organizer
      医学生物学電子顕微鏡技術学会第31回学術講演会
    • Place of Presentation
      名古屋市立大学
    • Year and Date
      2015-06-19
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] グルタルアルデヒド固定機序2015

    • Author(s)
      朴 杓允
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      国立京都国際会館 京都
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 末梢神経腫瘍におけるrudimentary ciliaの機能と役割2014

    • Author(s)
      朴 杓允
    • Organizer
      第9回中四国電子顕微鏡研究会
    • Place of Presentation
      高知大学医学部
    • Year and Date
      2014-10-04
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 化学固定理論とアーティファクト2014

    • Author(s)
      朴 杓允
    • Organizer
      植物電子顕微鏡サマーセミナ2014 (18th 細胞構造研究会@関東)
    • Place of Presentation
      帝京大学宇都宮キャンパス
    • Year and Date
      2014-08-26 – 2014-08-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 塩化ハフニウム水溶液の電子染色2014

    • Author(s)
      朴文守、朴杓允
    • Organizer
      植物電子顕微鏡サマーセミナ2014 (18th 細胞構造研究会@関東)
    • Place of Presentation
      帝京大学宇都宮キャンパス
    • Year and Date
      2014-08-26 – 2014-08-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Staining of non-isotope metals to cell structure in serial sections from animal2014

    • Author(s)
      Muranaka,Y., Park,M-S. Park,P
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      千葉幕張メッセ
    • Year and Date
      2014-05-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 電顕のアーティファクト形成機序2014

    • Author(s)
      朴 杓允
    • Organizer
      第17回細胞構造研究会
    • Place of Presentation
      神戸大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 植物病原糸状菌感染時において単子葉植物と双子葉植物で誘導されるパピラの形成機序に関する細胞学的比較解析.2013

    • Author(s)
      井上加奈子、宮田奈緒、毛呂恵、中屋敷均、朴杓允、池田健一
    • Organizer
      平成25年度日本植物病理学会関西部会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Autophagy-inducing condition during infection process of Magnaporthe oryzae.2013

    • Author(s)
      Inoue, K., Nakayashiki, H., and Ikeda, K.
    • Organizer
      10th International Congress of Plant Pathology
    • Place of Presentation
      中国北京
    • Related Report
      2013 Research-status Report
  • [Presentation] Attachment of airborne pathogens to their host; a potential target for disease control.2013

    • Author(s)
      Ikeda, K., Kitagawa, H., Shimoi, S., Inoue, K., Osaki, Y., Nakayashiki, H., Park, P.
    • Organizer
      10th International Congress of Plant Pathology
    • Place of Presentation
      中国北京
    • Related Report
      2013 Research-status Report
  • [Presentation] Multiple host adhesion factors in Magnaporthe oryzae ;potential target for disease control2013

    • Author(s)
      Ikeda, K., Kitagawa, H., Inoue, K., Shimoi, S., Osaki, Y., Park, P., and Nakayashiki, H.
    • Organizer
      6th International Rice Blast Conference 2013
    • Place of Presentation
      韓国Jeju
    • Related Report
      2013 Research-status Report
  • [Presentation] 透明人間(未固定成分)の可視化2013

    • Author(s)
      朴 杓允
    • Organizer
      中四国電顕研究会
    • Place of Presentation
      四国大学交流プラザ
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 超薄切片法と電子染色機序2013

    • Author(s)
      朴 杓允
    • Organizer
      第16回細胞構造研究会
    • Place of Presentation
      神戸大学
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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