Analysis and application of a new communication among oral bacteria using the conversion of autoinducer.

• Project/Area Number: 25450103
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Yamaguchi University
• Principal Investigator: Azakami Hiroyuki 山口大学, 農学部, 教授 (40263850)
• Co-Investigator(Renkei-kenkyūsha): NOIRI Yuichiro 大阪大学, 歯学研究科, 准教授 (50218286)
• Research Collaborator: Fariha Jasin Mansur ; MORISHIGE Natsumi ; TAKAHARA Sari ; Mohammad Minnatul Karim
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯周病原性細菌 / Eikenella corrodens / オートインデューサー / ポーリン / クオラムセンシング / quorum sensing / autoinducer / periodontal disease / 歯周病関連細菌

Outline of Final Research Achievements

Microorganisms communcate each other using various chemical compounds as language. Most pathogenic bacteria control their pathogenesis by these communication.Thus, if we can block their communication, we may apply it to treatment or prevention to many diseases. In this study, we found and cahracterized a new factor to block the bacterial communication. It is very interesting that this factor can block the wide range of communication among bacteria.

Research Products

• [Journal Article] Relationship between the risk for a shrimp allergy and freshness or cooking (2015)
  • Author(s): Masakatsu USUI, Akihito HARADA, Shinya YASUMOTO, Yoshimasa SUGIURA, Anri NISHIDAI, Maria IKARASHI, Honami TAKABA, Taiko MIYASAKI, Hiroyuki AZAKAMI, and Masakazu KONDO
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 未定 Issue: 10 Pages: 1698-1701
  • DOI: 10.1080/09168451.2015.1045830
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Unstable Mutant Lysozymes are Degraded through the Interaction with Calnexin Homologue Cne1p in Saccharomyces cerevisiae (2014)
  • Author(s): Azakami H. Uehara M. Matsuo R. Yamashita Y. Usui M. Kato A.
  • Journal Title: Biosci. Biotechol. Biochem Volume: 78 Issue: 7 Pages: 1263-1269
  • DOI: 10.1080/09168451.2014.918486
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of the tea catechin epigallocatechin gallate on Porphyromonas gingivalis biofilms (2014)
  • Author(s): Asahi Y. et al.
  • Journal Title: Jouranl of Applied Microbiology Volume: 116 Issue: 5 Pages: 1164-1171
  • DOI: 10.1111/jam.12458
  • Peer Reviewed
• [Journal Article] LuxS Affects Biofilm Maturation and Detachment of the Periodontopathogenic Bacterium Eikenella corrodens (2013)
  • Author(s): MM. Karim, T. Hisamoto, T. Matsunaga, Y. Asahi, Y. Noiri, S. Ebisu, A. Kato, H. Azakami
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 116 Issue: 3 Pages: 313-318
  • DOI: 10.1016/j.jbiosc.2013.03.013
  • NAID: 110009657226
  • Peer Reviewed
• [Journal Article] The Periodontopathogenic Bacterium <i>Eikenella corrodens</i> Produces an Autoinducer-2-Inactivating Enzyme (2013)
  • Author(s): MM. Karim, A. Nagao, FJ. Mansur, T. Matsunaga, Y. Akakabe, Y. Noiri, S. Ebisu, A. Kato, H. Azakami
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 5 Pages: 1080-1085
  • DOI: 10.1271/bbb.130047
  • NAID: 10031177600
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Contribution of Structural Reversibility to the Heat Stability of the Tropomyosin Shrimp Allergen (2013)
  • Author(s): Masakatsu USUI, Akihito HARADA, Takayuki ISHIMARU, Emiri SAKUMICHI, Fumihiko SARATANI, Chiho SATO-MINAMI, Hiroyuki AZAKAMI, Taiko MIYASAKI, Ken'ichi HANAOKA
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 5 Pages: 948-953
  • DOI: 10.1271/bbb.120887
  • NAID: 10031177579
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Presentation] Autoinducer-inactivation mechanism in periodontopathogenic bacterium Eikenella corrodens (2016)
  • Author(s): Fariha Jasin Mansur、阿座上弘行
  • Organizer: 日本細菌学会
  • Place of Presentation: 大阪国際交流センター（大阪府大阪市）
  • Year and Date: 2016-03-24
• [Presentation] 歯周病原性細菌Eikenella corrodensにおけるオートインデューサー不活化機構の解析 (2015)
  • Author(s): Fariha Jasin Mansur、森重なつみ、飯田亮平、島谷雅文、阿座上弘行
  • Organizer: 日本生物工学会
  • Place of Presentation: 城山観光ホテル（鹿児島県鹿児島市）
  • Year and Date: 2015-10-28
• [Presentation] 歯周病原性細菌Eikenella corrodensにおけるオートインデューサー不活化機構の解析 (2015)
  • Author(s): 阿座上弘行、Fariha Jasin Mansur、森重なつみ
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 土浦亀城プラザ（茨城県土浦市）
  • Year and Date: 2015-10-19
• [Presentation] 歯周病原性細菌Eikenella corrodensにおけるオートインデューサー不活化機構の解析 (2015)
  • Author(s): Fariha Jasin Mansur、森重なつみ、飯田亮平、島谷雅文、阿座上弘行
  • Organizer: 日本農芸化学会中四国・西日本合同支部大会
  • Place of Presentation: 愛媛大学（愛媛県松山市）
  • Year and Date: 2015-09-18
• [Presentation] Autoinducer-2 inactivation by outer membrane porin in periodontopathogenic bacterium Eikenella corrodens (2015)
  • Author(s): Fariha Jasin Mansur、森重なつみ、野杁由一郎、阿座上弘行
  • Organizer: 日本農芸化学会
  • Place of Presentation: 岡山大学 （岡山市）
  • Year and Date: 2015-03-29
• [Presentation] 歯周病原性細菌Eikenella corrodensにおけるゲノム再編による溶血活性の発現 (2015)
  • Author(s): 高原沙里、下川床愛、野杁由一郎、阿座上弘行
  • Organizer: 日本農芸化学会
  • Place of Presentation: 岡山大学 （岡山市）
  • Year and Date: 2015-03-27
• [Presentation] 歯周病原性細菌Eikenella corrodensの外膜ポーリンによるオートインデューサー２の不活化機構 (2014)
  • Author(s): 森重なつみ、Fariha Jasin Mansur、飯田亮平、野杁由一郎、阿座上弘行
  • Organizer: 日本生物工学会
  • Place of Presentation: 札幌コンベンションセンター （札幌市）
  • Year and Date: 2014-09-10
• [Presentation] 歯周病原性細菌Eikenella corrodensのX-プロリルアミノペプチダーゼが溶血に関与する？ (2014)
  • Author(s): 高原沙里、下川床愛、野杁由一郎、阿座上弘行
  • Organizer: 日本生物工学会
  • Place of Presentation: 札幌コンベンションセンター （札幌市）
  • Year and Date: 2014-09-10
• [Presentation] Purification and characterization of autoinducer-2 inactivating enzyme in periodontopathogenic bacterium Eikenella corrodens (2014)
  • Author(s): 〇Fariha Manusr、Mohammad Karim、Yuichiro Noiri、Hiroyuki Azakami
  • Organizer: IUMS2014
  • Place of Presentation: モントリオール (カナダ）
  • Year and Date: 2014-07-30
• [Presentation] 歯周病原性細菌Eikenella corrodensのポーリンがオートインデューサーの不活化に関与する (2014)
  • Author(s): 阿座上弘行、Mansur Jasin、野杁由一郎、加藤昭夫
  • Organizer: 日本農芸化学会
  • Place of Presentation: 明治大学生田キャンパス（川崎市）
• [Presentation] Involvement of porin in inactivation of autoinducer 2 in Eikenella corrodens (2014)
  • Author(s): Mansur Jasin、野杁由一郎、阿座上弘行
  • Organizer: 日本細菌学会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
• [Presentation] The periodontopathogenic bacterium Eikenella corrodens produces an autoinducer-2-inactivating enzyme (2013)
  • Author(s): Hiroyuki Azakami, Mansur Jasin
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 鹿児島大学（鹿児島市）
• [Presentation] 歯周病原性細菌の可溶性ポーリンがオートインデューサーを不活化する？ (2013)
  • Author(s): 阿座上弘行、Mansur Jasin、Karim Minnatul、野杁由一郎
  • Organizer: 日本生物工学会
  • Place of Presentation: 広島国際会議場（広島市）
• [Presentation] Eikenella corrodens produces an autoinducer-2-inactivating enzyme (2013)
  • Author(s): Fariha Jasin Mansur, Mohammad Minnatul Karim, Yuichiro Noiri, Shigeyuki Ebisu, Hiroyuki Azakami
  • Organizer: Nobel Conference on Biofilm formation, its clinical impact and potential treatment
  • Place of Presentation: Stockholm, Sweden
• [Presentation] LuxS affects biofilm maturation and detachment of the periodontopathogenic bacterium Eikenella corrodens (2013)
  • Author(s): Mohammad Minnatul Karim, Fariha Jasin Mansur, Tatsunori Hisamoto, Tetsuro Matsunaga, Yoko Asahi, Yuichiro Noiri, Shigeyuki Ebisu, Akio Kato, Hiroyuki Azakami
  • Organizer: Nobel Conference on Biofilm formation, its clinical impact and potential treatment
  • Place of Presentation: Stockholm, Sweden
• [Presentation] The Periodontopathogenic Bacterium Eikenella corrodens Produces an Autoinducer-2-Inactivating Enzyme (2013)
  • Author(s): Hiroyuki Azakami, Karim Minnatul, Mansur Jasin, Yuichiro Noiri, Shigeyuki Ebisu
  • Organizer: Gordon Conference (Bacterial Adhesion & Signal Transduction)
  • Place of Presentation: Newport, USA
• [Presentation] 歯周病関連細菌Eikenella corrodensのAI-2不活化酵素の精製と解析 (2013)
  • Author(s): 阿座上弘行、Karim Minnatul、Mansur Jasin、野杁由一郎、恵比須繁之
  • Organizer: Bacterial Adherence & Biofilm学術集会
  • Place of Presentation: 東京ガーデンパレス（東京都文京区）
• [Book] 「細胞間コミュニケーションと口腔細菌バイオフィルム」特集『バイオフィルム研究の最前線』 (2015)
  • Author(s): 阿座上弘行、恵比須繁之
  • Total Pages: 180
  • Publisher: 医薬ジャーナル社