| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
For generation of the cell factories for bio-based organic acid production, functional analyses of genes encoding putative mitochondrial citrate transport proteins in citric acid-producing Aspergillus niger were performed. Screening of microorganisms capable of assimilating citric acid as a sole carbon source was also performed, and a novel aconitate isomerase was obtained. trans-Aconitic acid was produced successfully from citric acid by utilization of recombinant E. coli cells heterologously expressing the novel aconitate isomerase gene as biocatalysts for the whole-cell reaction. There is some possibility that heterologous expression of genes encoding novel and specific enzymes related to citrate metabolism in citric acid-producing A. niger as a host and improvement of citrate transport system by genetic engineering will open the way for generation of the cell factories for bio-based organic acid production.
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